Study on the Antileukemic Effect of Galla Rhois

  • Kim, Myung-Wan (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Kun-Jung (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yun, Yong-Gab (Department of Oriental Medical Prescrition, College of Oriental Medicine, Wonkwang University) ;
  • Han, Dong-Min (Division of Life Science, College of Natural Sciences, Wonkwang University) ;
  • Kim, Won-Sin (Division of Life Science, College of Natural Sciences, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • Published : 2005.02.25

Abstract

Galla Rhois is a nest of parasitic bug, Mellaphis chinensis Bell, in Rhus chinensis Mill. Galla Rhois has been used for the therapy of diarrhea, peptic ulcer, hemauria, etc., that showed various antiinflammatory activity, and other biological properties. We studied the effect of Galla Rhois water extract(GRWE). The cytotoxic activity of GRWE in HL-60 cells was increased in a concentration-dependent manner. GRWE was cytotoxic to HL-60 cells, with $IC_50$ of $100{\mu}g/m{\ell}$. Treatment of GRWE to HL-60 cells showed the fragmentation of DNA in a concentration manner, suggesting that these cells underwent apoptosis. In addition, the flow cytometric analysis revealed GRWE concentration-dependently increased apoptotic cells with hypodiploid DNA content and arrested G1 phase of cell cycle. These results indicate that GRWE may have a possibility of potential anticancer activities. Treatment of HL-60 cells with GRWE was induced activation of caspase-3, caspase-8 and proteolytic cleavage of poly(ADP-ribose) polymerase. Also, caspase-3 was directly activated via caspase-8 activation. GRWE also caused the release of cytochrome c from mitochondria into the cytosol. GRWE-induced cytochrome c release was mediated by caspase-8-dependent cleavage of Bid and Bax translocation. These results suggest that caspase-8 mediates caspase-3 activation and cytochrome c release during GRWE-induced apoptosis in HL-60 cells.

Keywords

References

  1. Scott JD, Williams RM. Chemistry and biology of the tetrahydroisoquinoline antitumor antibiotics. Chem Rev. May;102(5):1669-730, 2002. https://doi.org/10.1021/cr010212u
  2. Kalechman Y, Longo DL, Catane R, Shani A, Albeck M, Sredni B. Synergistic anti-tumoral effect of paclitaxel (Taxol)+AS101 in a murine model of B16 melanoma: association with ras-dependent signal-transduction pathways. Int J Cancer. Apr 15;86(2):281-8, 2000.
  3. Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J, Terce F. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. Mar 1;60(5):1426-33, 2000.
  4. Ling YH, Yang Y, Tomos C, Singh B, Perez-Soler R. Paclitaxel-induced apoptosis is associated with expression and activation of c-Mos gene product in human ovarian carcinoma SKOV3 cells. Cancer Res. Aug 15;58(16):3633-40, 1998.
  5. Nieves-Neira W, Pommier Y. Apoptotic response to camptothecin and 7-hydroxystaurosporine (UCN-01) in the human breast cancer cell lines of the NCI Anticancer Drug Screen: multifactorial relationships with topoisomerase I, protein kinase C, Bcl-2, p53, MDM-2 and caspase pathways. Int J Cancer. Jul 30;82(3):396-404, 1999.
  6. Piazza GA, Rahm AL, Krutzsch M, Sperl G, Paranka NS, Gross PH, Brendel K, Burt RW, Alberts DS, Pamukcu R, et al. Antineoplastic drugs sulindac sulfide and sulfone inhibit cell growth by inducing apoptosis. Cancer Res. Jul 15;55(14):3110-6, 1995.
  7. Evan G, Littlewood T. A matter of life and cell death. Science. Aug 28;281(5381):1317-22 1998.
  8. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. Aug 28;281(5381):1312-6, 1998.
  9. Piao W, Yoo J, Lee DK, Hwang HJ, Kim JH. Induction of G(2)/M phase arrest and apoptosis by a new synthetic anti-cancer agent, DW2282, in promyelocytic leukemia (HL-60) cells. Biochem Pharmacol. Dec 1;62(11):1439-47, 2001.
  10. Kim R, Tanabe K, Uchida Y, Emi M, Inoue H, Toge T. Current status of the molecular mechanisms of anticancer drug-induced apoptosis, The contribution of molecular-level analysis to cancer chemotherapy. Cancer Chemother Pharmacol. 2002 Nov;50(5):343-52. Epub Oct 03, 2002.
  11. Kroemer G. Mitochondrial control of apoptosis: an overview. Biochem Soc Symp. 66:1-15, 1999.
  12. Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME. Two CD95 (APO-1/Fas) signaling pathways. EMBO J. Mar 16;17(6):1675-87, 1998.
  13. Susin SA, Lorenzo HK, Zarnzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, Larochette N, Goodlett DR, Aebersold R, Siderovski DP, Penninger JM, Kroemer G. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature. Feb 4;397(6718):441-6, 1999.
  14. Kroemer G, Zamzami N, Susin SA. Mitochondrial control of apoptosis. Immunol Today. Jan;18(1):44-51, 1997.
  15. Mignotte B, Vayssiere JL. Mitochondria and apoptosis. Eur J Biochem. Feb 15;252(1):1-15, 1998.
  16. Zou H, Li Y, Liu X, Wang X. An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem. Apr 23;274(17):11549-56, 1999.
  17. Sun XM, MacFarlane M, Zhuang J, Wolf BB, Green DR, Cohen GM. Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J Biol Chem. Feb 19;274(8):5053-60, 1999.
  18. Luo X, Budihardjo I, Zou H, Slaughter C, Wang X. Bid, a Bc12 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell. Aug 21;94(4):481-90, 1998.
  19. Li H, Zhu H, Xu CJ, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. Aug 21;94(4):491-501, 1998.
  20. Hsu YT, Youle RJ. Bax in murine thymus is a soluble monomeric protein that displays differential detergent- induced conformations. J Biol Chem Apr 24;273(17):10777-83, 1998.
  21. Murphy KM, Streips UN, Lock RB. Bax membrane insertion during Fas(CD95)-induced apoptosis precedes cytochrome c release and is inhibited by Bcl-2. Oncogene. Oct 28;18(44):5991-9, 1999.
  22. Gross A, McDonnell JM, Korsmeyer SJ. BCL-2 family members and the mitochondria in apoptosis. Genes Dev. Aug 1;13(15):1899-911, 1999.
  23. Finucane DM, Bossy-Wetzel E, Waterhouse NJ, Cotter TG, Green DR. Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL. J Biol Chem. Jan 22;274(4):2225-33, 1999.
  24. Reed JC. Cytochrome c: can't live with it--can't live without it. Cell. Nov 28;91(5):559-62, 1997.
  25. Kluck RM, Bossy-Wetzel E, Green DR, Newmeyer DD. The release of cytochrome c from mitochondria: a primary site for Bc1-2 regulation of apoptosis. Science. Feb 21;275(5303):1132-6, 1997.
  26. Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J, Peng TI, Jones DP, Wang X. revention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science. Feb 21;275(5303):1129-32, 1997.
  27. Zhuang J, Dinsdale D, Cohen GM. Aoptosis, in human monocytic THP.1 cells, results in the release of cytochrome c from mitochondria prior to their ultracondensation, formation of outer membrane discontinuities and reduction in inner membrane potential. Cell Death Differ. Nov;5(11):953-62, 1998.
  28. Li H, Zhu H, Xu CJ, Yuan J. leavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. Aug 21;94(4):491-501, 1998.
  29. Luo X, Budihardjo I, Zou H, Slaughter C, Wang X. id, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell. Aug 21;94(4):481-90, 1998.
  30. Wang K, Yin XM, Chao DT, Milliman CL, Korsmeyer SJ. ID: a novel BH3 domain-only death agonist. Genes Dev. Nov 15;10(22):2859-69, 1996.
  31. Pae HO, Oh H, Yun YG, Oh GS, Jang SI, Hwang KM, Kwon TO, Lee HS, Chung HT. mperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae). induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 Cells. Pharmacol Toxicol. Jul;91(1):40-8, 2002.
  32. Desagher S, Osen-Sand A, Nichols A, Eskes R, Montessuit S, Lauper S, Maundrell K, Antonsson B, Martinou JC. id-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis. J Cell Biol. Mar 8;144(5):891-901, 1999.
  33. Eskes R, Desagher S, Antonsson B, Martinou JC. Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane. Mol Cell Biol. Feb;20(3):929-35, 2000.
  34. Korsmeyer SJ, Wei MC, Saito M, Weiler S, Oh KJ, Schlesinger PH Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c. Cell Death Differ. Dec;7(12):1166-73, 2000.
  35. Ruffolo SC, Breckenridge DG, Nguyen M, Goping IS, Gross A, Korsmeyer SJ, Li H, Yuan J, Shore GC. BID-dependent and BID-independent pathways for BAX insertion into mitochondria. Cell Death Differ. Nov;7(11):1101-8, 2000.
  36. King KL, Cidlowski JA. Cell cycle regulation and apoptosis. Annu Rev Physiol. 60:601-17, 1998. https://doi.org/10.1146/annurev.physiol.60.1.601
  37. Weinberg RA. E2F and cell proliferation: a world turned upside down. Cell. May 17;85(4):457-9, 1996.
  38. Picksley SM, Lane DP. p53 and Rb: their cellular roles. Curr Opin Cell Biol. Dec;6(6):853-8, 1994.
  39. Hengartner MO. The biochemistry of apoptosis. Nature. Oct 12;407(6805):770-6, 2000.
  40. Ashkenazi A, Dixit VM. Apoptosis control by death and decoy receptors. Curr Opin Cell Biol. Apr;11(2):255-60, 1999.
  41. Muzio M, Stockwell BR, Stennicke HR, Salvesen GS, Dixit VM. An induced proximity model for caspase-8 activation. J Biol Chem. Jan 30;273(5):2926-30, 1998.
  42. Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization. Mol Cell. Jun;1(7):949-57, 1998.
  43. Muller M, Strand S, Hug H, Heinemann EM, Walczak H, Hofmann WJ, Stremmel W, Krammer PH, Galle PR. Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-l/Fas) receptor/ligand system and involves activation of wild-type p53. J Clin Invest. Feb 1;99(3):403-13, 1997.
  44. Jurgensmeier JM, Xie Z, Deveraux Q, Ellerby L, Bredesen D, Reed JC. Bax directly induces release of cytochrome c from isolated mitochondria. Proc Natl Acad Sci USA. Apr 28;95(9):4997-5002, 1998.