Antifungal Activity of Chloroform Extract from Riccardia marginata on the Dermatophytic Fungus Trichophyton mentagrophytes

  • Na, Young-Soon (Department of Beauty, Konyang University) ;
  • Lee, Hong (Division of Bionano Chemistry, Wonkwang University) ;
  • Kim, Myung-Ju (Department of Skin & Beauty, Kwangju Health Science College) ;
  • Oh, Hyun-Ju (Department of Beauty Art, Honam University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Institute of Basic Natural Sciences, Wonkwang University)
  • Published : 2005.04.25


The effects of chloroform extract from Riccardia marginata on antifungal activity were investigated. The crude chloroform extract of R. marginata inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (4 mm inhibition zone at $150\;{\mu}g/disc$) and the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (6 mm inhibition zone at $150\;{\mu}g/disc$), and inactive to P388 murine leukaemia cells ATCC CCL 46 P388D1, ($IC_{50}\;>25,000\;{\mu}g/mL$ at $150\;{\mu}g/disc$). This crude chloroform extract of R. marginata showed strong antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes.



  1. Schofield, W.B. Introduction to Bryology MacMillan Publishing Copy. New York, 1985.
  2. Asakawa, Y. In progress in the Chemistry of Organic Natural Products ; Herz, W., Kirby, G. W., Moore, R. E., Steglich, W., Tamm, C, Eds. Springer Vienma, Vol. 65, 1-525, 1995.
  3. Zinsmeister, H.D., Becker, H, Eicher, T. Bryophytes, a Source of Biologically Active, Naturally Occurring Material? Angew Chem Int Ed 30, 130-147, 1991.
  4. Asakawa, Y. Recent advances in phytochemistry of bryophytes-acetogenins, terpenoids and bis(bibenzyl)s from selected Japanese, Taiwanese, New Zealand, Argentinean and European liverworts. Phytochemistry 56, 297-312, 2001.
  5. New Zealand Plant Names Database. Landcare Research, New Zealand: 2000; accessed 14 August 2003.
  6. Allison, K.W., Child, J. The Liverworts of New Zealand; University of Otago Press: Dunedin, 1975.
  7. Perry, N. B., Foster, L. M. Sesquiterpene/quinol from a New Zealand liverwort, Riccardia crassa. J Nat Prod 58, 1131-1135, 1995.
  8. Toyota, M., Asakawa, Y. Sesquiterpene derivatives and a norsesquiterpenoid from the liverworts Riccardia crassa and Porella caespitans. Phytochemistry 32, 137-140, 1990.
  9. Benesova, V., Samek, Z., Herout, V., Sorrn, F. Plant substances. XXIX. Isolation and structure of two new indole alkaloids from Riccardia sinuata. Collect Czech Chem Commun 34, 1807-1809, 1969.
  10. Benesova, V., Herout, V., Sorm, F. Plant substances. XXX. Components of liverworts Aneura pinguis, Riccardia sinuaia, R. incuroata, and Conocephalum conicum. Collect Czech Chem Commun 34, 1810-1814, 1969.
  11. Asakawa, Y., Toyota, M., Matsuda, R., Takikawa, K., Takemoto, T. Chemosystematics of bryophytes. Part 15. Distribution of novel cyclic bisbibenzyls in Marchantia and Riccardia species. Phytochemistry 22, 1413-1415, 1983.
  12. Asakawa, Y., Toyota, M., Taira, Z., Takemoto, T., Kido, M., S. Gray. Riccardin A and riccardia B two novel cyclic bis(bibenzyls) possessing cytotoxicity from the liverwort Riccardia multifida (L.) J Org Chem 48, 2164-2167, 1983.
  13. Dienes, Z., Nogradi, M., Vermes, B., Kajtar-Peredy, M. Synthesis of marchantin I, a macrocyclic bis(bibenzyl ether) from Riccardia multifida. Liebigs Ann Chem 1141-1143, 1989.
  14. Kurata, K., Amiya, T. A new bromophenol from the red alga Polysiphonia urceolata. Bull Chem Soc Jpn 53, 2020-2022, 1980
  15. Lorimer, S.D., Barns, G., Evans, AC, Foster, L.M., May, B.C.H, Perry, N.B., Tangney, R.5. Cytotoxicity and antimicrobial activity of plants from New Zealand's subantarctic islands. Phytomed 2, 327-333, 1996.
  16. Schroeder, A.C. Hughes, R.C. Jr., Bloch, A. Synthesis and biological effects of acyclic pyrimidine nucleoside analogues. J Med Chem 24, 1078-1083, 1981.
  17. Baek, S. H. Lim, J. A., Kwag, J. S., Lee, H. O., Chun, H. J., Lee, J. H., Perry, N. B. Screening for biological activity of crude extract and bioactive fractions from Brochyglottis monroi, Kor J Orien Physiol & Pathol 17(3) : 826-828, 2003.