대한약침학회지 (Journal of Pharmacopuncture)

  • 제8권3호
  • /
  • Pages.5-9
  • /
  • 2005
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  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
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형광염색을 이용한 혈관내봉한관의 관찰

Fluorescent Method for Observing Intravascular Bonghan Duct

  • 이병천 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 백구연 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 정현민 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 성백경 (서울대학교 자연과학대학 물리학부 한의학물리연구실) ;
  • 소경순 (세명대학교 한의과대학) ;
  • 강대인 (세명대학교 한의과대학) ;
  • 소광섭 (서울대학교 자연과학대학 물리학부 한의학물리연구실)
  • Lee, Byung-Cheon (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Baik, Ku-Youn (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Johng, Hyeon-Min (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Sung, Baeck-Kyoung (Biomedical Physics Laboratory, School of Physics, Seoul National University) ;
  • Soh, Kyung-Soon (College of Oriental medicine, Semyung University) ;
  • Kang, Dae-In (College of Oriental medicine, Semyung University) ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory, School of Physics, Seoul National University)
  • 발행 : 2005.12.30
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초록

미분간섭현미경법과 아크리딘-오렌지(acridine-orange) 형광 염색을 이용하여 쥐 혈관 내의 실 모양 구조물을 관찰하였다. 공초점현미경법과 헤마톡실린-에오신(hematoxylin-eosin) 염색을 통해 피브린, 모세혈관, 소정맥, 소동맥 혹은 림프관의 핵 분포와 뚜렷이 구별되는 혈관 내 실 모양 구조물의 핵 분포 패턴을 얻어낼 수 있었다. 이 실 모양 구조물의 생리적 기능을 침술과 연관하여 논의하였다. 특히, 이 실 모양 관을 통한 약물의 흐름이 약침의 기전에 해당할 것이라는 가설을 제기하였다.

Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.

키워드

참고문헌

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  5. Baik KY, Lee J, Lee BC, et al. (2004) Acupuncture Meridian and Intravascular Bonghan Duct. Key Eng. Mat., accepted https://doi.org/10.4028/www.scientific.net/KEM.277-279.125
  6. Baik, K. Y., Park, E. S., Lee, B. C., Kim, H. K., Cho, S. I., Jiang, X, Shin, H. S., Choi, C. H., Yi, S. H., Johng H. M., Nam, T. J., Soh, K. S., Cheun, B. S., Park, D. H., Nahm, Y. S., Yoon, Y. S., Lee, I. S., Ahn, S. Y., and Soh, K. S., (2004) Histology of threadlike structure inside blood vessel. J. Intl. Soc. Life Info. Sci. 22, to be published.
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