Protection of Amyloid ${\beta}$ Protein (25-35)-induced Neuronal Cell Damage by Methanol Extract of New Stem of Phyllostachys nigra Munro var. henonis Stapf in Cultured Rat Cortical Neuron

  • Ban, Ju-Yeon (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.) ;
  • Cho, Soon-Ock (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.) ;
  • Kwon, Soon-Ho (College of Agriculture & Life-Sci., Kyungpook Natl. Univ.) ;
  • Kim, Jin-Bae (College of Oriental Medicine, Kyung Hee Univ.) ;
  • Song, Nak-Sul (National Institute of Crop Science, RDA.) ;
  • Bae, Ki-Whan (College of Pharmacy, Chungnam Natl. Univ.) ;
  • Song, Kyung-Sik (College of Agriculture & Life-Sci., Kyungpook Natl. Univ.) ;
  • Seng, Yeon-Hee (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.)
  • 발행 : 2005.04.30

초록

Caulis Bambusae in Taenia is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of Caulis Bambusae in Taenia (CB) from Phyllostachys nigra Munro var. henonis Stapf (Gramineae) on amyloid ${\beta}$ protein (25-35) $(A{\beta}\;(25-35))$, a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. CB, over a concentration range of $10-50{\mu}g/{\mu}l$, inhibited the $A{\beta}\;(25-35)\;(10\;{\mu}M)$-induced neuronal cell death, as assessed by a 3-[4,5-dimethyIthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. CB $(50\;{\mu}g/{\mu}l)$ inhibited glutamate release into medium induced by $10\;{\mu}M\;A{\beta}$, (25-35) which was measured by HPLC. Pretreatment of CB $(50\;{\mu}g/{\mu}l)$ inhibited $10{\mu}M\;A{\beta}$ (25-35)-induced elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, fluo-4 AM, and generation of reactive oxygen species. These results suggest that CB prevents $A{\beta}$ (25-35)-induced neuronal ell damage in vitro.

키워드

참고문헌

  1. J. Neurosci. Res. v.41 ${\beta}$-Amyloid peptide fragment 25-35 potentiates the calcium-dependent release of excitatory amino acids from depolarized hippocampal slices Arias, C.;Arrieta, I.;Tapia, R.
  2. Eur. J. Pharmacol. Blockade of 5-$HT_3$ receptor with MDL72222 and Y25130 reduces amyloid ${\beta}$ protein (25-35)-induced neurotoxicity in cultured rat cortical cells Ban, J.Y.;Seong, Y.H.
  3. Cell v.77 Hydrogen peroxide mediates amyloid beta protein toxicity Behl, C.;Davis, J.B.;Lesley, R.;Schubert, D.
  4. Brain Res. v.931 Induction of cytochrome c-mediated apoptosis by amyloid ${\beta}$ 25-35 requires functional mitochondria Cardoso, S.M.;Swerdlow, R.H.;Oliveira, C.R.
  5. Brain Res. v.715 Neuropathology of synthetic ${\bets}$-amyloid peptide analogs in vivo Chen, S.Y.;Harding, J.W.;Barnes, C.D.
  6. Neurobiol. Aging v.13 Beta-amyloid neurotoxicity: a discussion of in vitro findings Cotman, C.W.;Pike, C.J.;Copani, A.
  7. Mol. Brain Res. v.76 ${\beta}$-Amyloid-induced calcium influx induces apoptosis in culture by oxidative stress rather than tau phosphorylation Ekinci, F.J.;Linsley, M.D.;Shea, T.B.
  8. J. BioI. Chem. v.274 Activation of the L voltage-sensitive calcium channel by mitogen-activated protein (MAP) kinase following exposure of neuronal cells to beta-amyloid. MAP kinase mediates ${\beta}$-amyloid-induced neurodegeneration Ekinci, F.J.;Malik, K.U.;Shea, T.B.
  9. J. Neurosci. Methods v.19 Amino acid neurotransmitters in postmortem human brain analyzed by high performance liquid chromatography with electrochemical detection Ellison, D.W.;Beal, M.F.;Martin, J.B.
  10. Brain Res. v.732 Determination of intracellular $Ca^{2+}$ concentration can be useful tool to predict neuronal damage and neuroprotective properties of drugs Ferger, D.;Krieglstein, J.
  11. Wan Mi-zhai's Zhu Ru Tang (Caulis Bambusae Decoction) & fear and fright Flaws, B.
  12. Funct. Neurol. v.8 ${\beta}$-Amyloid neurotoxicity Forloni, G.
  13. Exp. Neurol. v.128 Secreted forms of ${\beta}$-amyloid precursor protein protect hippocampal neurons against amyloid ${\beta}$-peptide-induced oxidative injury Goodman, Y.;Mattson, M.P.
  14. Brain Res. v.691 Neurodegeneration mediated by glutamate and peptide: a comparison and possible interaction Gray, C.W.;Patel, A.J.
  15. Prog. Neuropsychopharmacol. BioI. Psychiatry v.23 Neuroprotective approaches in experimental models of ${\beta}$-amyloid neurotoxicity: relevance to Alzheimer's disease Harkany, T.;Hortobagyi, T.;Sasvari, M.;Konya, C.;Penke, B.;Luiten, P.G.;Nyakas, C.
  16. Nat. Med. v.4 Accelerated Alzheimer-type phenotype in transgenic mice carrying both mutant amyloid precursor protein and presenilin I transgenes Holcomb, L.;Gordon, M.N.;McGowan, E.;Yu, X.;Benkovic, S.;Jantzen, P.;Wright, K.;Saad, I.;Mueller, R.;Morgan, D.;Sanders, S.
  17. CNS Drugs v.11 Disturbances of calcium homeostasis in ischemic stroke: Therapeutical implication Horn, J.;Brouwers, P.J.A.M.;Limburg, M.
  18. Neuron v.15 Age-related CNS disorder and early death in transgenic FVB/N mice overexpressing Alzheimer amyloid precursor proteins Hsiao, K.H.;Borchelt, D.R.;Olson, K.;Johannsdottir, R.;Kitt, C.;Yunis, W.;Xu, S.;Eckman, C.;Younkin, S.;Price, D.;Iadecola, C.;Clark, H.B.;Carlson, G.
  19. Biochem. J. v.311 The toxicity in vitro of ${\beta}$-amyloid protein Iversen, L.L.;Mortishier-Smith, R.J.;Pollack, S.J.;Shearman, M.S.
  20. J. BioI. Chem. v.274 Multiple pathways of apoptosis in PC12 cells. CrmA inhibits apoptosis induced by beta-amyloid Ivins, K.J.;Ivins, J.K.;Sharp, J.P.;Cotman, C.W.
  21. J. Plant Biotechnol. v.5 Transdermal pain palliative agents containing Chinese medicinal herbs Jia, W.;Gao, W.;Cui, N.;Tang, L.;Liu, V.J.K.;Yang, J.;Qiu, Y.;Su, M.;Yu, X.
  22. Brain Res. v.533 ${\beta}$-Amyloid protein increases the vulnerability of cultured neurons to excitotoxic damage Koh, J.Y.;Yang, L.L.;Cotman, C.W.
  23. Pharmacol. Res. v.51 no.3 Stimulation of $5-HT_{1A}$ receptor with 8-0H-DPAT inhibits hydrogen peroxideinduced neurotoxicity in cultured rat cortical cells Lee, H.J.;Ban, J.Y.;Cho, S.O.;Seong, Y.H.
  24. J. BioI. Chem. v.273 Phosphatidic acid-induced elevation of intracellular $Ca^{2+}$ is mediated RhoA and $H_2O_2$ in rat-2 fibroblasts Lee, Z.W.;Kweon, S.M.;Kim, B.C.;Leem, S.H.;Shin, I.C.;Kim, J.H.;Ha, K.S.
  25. J. Neurosci. v.22 NMDA receptor antagonists disinhibit rat posterior cingulate and retrosplenial cortices: a potential mechanism of neutoxicity Li, Q.;Clark, S.;Lewis, D.V.;Wilson, W.A.
  26. Prog. Neurobiol. v.62 The role of oxidative stress in the toxicity induced by amyloid ${\beta}$-peptide in Alzheimer's disease Miranda, S.;Opazo, C.;Larrondo, L.F.;Munoz, F.J.;Ruiz, F.;Leighton, F.;Inestrosa, N.C.
  27. Brain Res. Bull. v.45 ${\beta}$-Amyloid-induced cholinergic denervation correlates with enhanced nitric oxide synthase activity in rat cerebral cortex: Reversal by NMDA receptor blockade O'Mahony, S.;Harkany, T.;Rensink, A.A.;Abraham, I.;De Jong, G.I.;Varga, J.L.;Zarand, M.;Penke, B.
  28. Neurosci. Res. v.37 Oxidative glutamate toxicity involves mitochondrial dysfunction and perturbation of intracellular $Ca^{2+}$homeostasis Pereira, C.F.;Oliveira, C.R.
  29. Pharmacopoeia of the people's Republic of China
  30. Prog. Neuro. Psychophrmacol. v.23 Neuroprotective approaches in experimental models of ${\beta}$-amyloid neurotoxicity: relevance to Alzheimer's disease Tibor, H.;Maria, S.;Csaba, K.;Botond, P.;Paul, G.M.;Luiten;Csaba, N.
  31. Brain Res. v.639 Amyloid ${\beta}$ protein-induced neuronal cell death: neurotoxic properties of aggregated amyloid ${\beta}$ protein Ueda, K.;Fukui, Y.;Kageyama, H.
  32. J. Neurochem. v.68 Amyloid ${\beta}$ protein potentiates $Ca^{2+}$ influx through L-type voltage sensitive $Ca^{2+}$ channels: a possible involvement of free radicals Ueda, K.;Shinohara, S.;Yagami, T.;Asakura, K.;Kawasaki, K.
  33. Neuroscience v.92 ${\beta}$-Amyloid peptide Yan, X.T.;Qiao, J.T.;Dou, Y.;Qiao, Z.D.
  34. Medical Introductory v.21 Market and development status of pain-relief drugs Zhang, Y.F.;Fu, X.;Qing, H.J.