Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Selection of PCR Markers and Its Application for Distinguishing Dried Root of Three Species of Angelica

  • Published : 2005.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

An analysis of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) was performed with three Angelica species (A. gigas Nakai, A. sinensis (Olive.) Diels and A. acutiloba Kitag) in an effort to distinguish between members of these three species. Two arbitrary primers (OPC02, OPD11) out of80 primers tested, produced 17 species-specific fragments among the three species. Eight fragments were specific for A. sinensis, four fragments specific for A. gigas, five specific for A. acutiloba. When primers OPC02 and OPD11 were used in the polymerase chain reaction, RAPD-PCR fragments that were specific for each of the three species were generated simultaneously. Primer OPC02 produced eight species-specific fragments: four were specific for A. sinensis, one for A. gigas, and three for A. acutiloba. Primer OPD11 produced nine speciesspecific fragments: four for A. sinensis, three for A. gigas, and two for A. acutiloba. The RAPD-PCR markers that were generated with these two primers should rapidly identify members of the three Angelica species. The consistency of the identifications made with these species-specific RAPD-PCR markers was demonstrated by the observation that each respective marker was generated from three accessions of each species, all with different origins. We also performed the RAPD-PCR analysis with the dried Angelica root samples that randomly collected from marketed and from the OPC02 primer, obtained a A. gigasspecific band and the band were cloned and sequenced.

Keywords

References

  1. BMC Genetics v.5 Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays Arvind, K.A.;Nagaraja, G.M.;Naik, G.V.;Kanginakudru, S.;Thangavelu, K.;Nagaraju, J.
  2. Korean J. Medicinal Crop Sci. v.10 no.1 Selection of RAPD marker to discriminate the bolting-resistant varieties and commercial dried medicinal materials of Angelica species Bang, K.H.;Yu, H.S.;Koo, D.H.;Cho, J.H.;Park, H.W.;Seong, N.S.;Park, S.I.;Kim, H.S.
  3. BMC Plant BioI. v.1 Molecular distinction amongst varieties of Mulberry using RAPD and DAMD profiles Bhattacharya, E.;Ranade, S.A.
  4. Chinese Medical Herbology and Pharmacology Chen, J.K.;Chen, T.T.
  5. Phytochem. Bull. v.19 A rapid DNA isolation procedure for small quantities of fresh leaf tissue Doyle, J.J.;Doyle, J.S.
  6. Crop Science v.43 Development of Species-Specific SCAR Markers in Bentgrass Elizabeth, A.S.;Michael, D.C.;Jung, G.H.
  7. J. Hort. Sci. v.69 Identification of red raspberry cultivars and an assessment to their relatedness using fingerprintings produced by random primers Graham, J.;McNicol, R.J.;Greig, K.;Van de ven, W.T.G.
  8. International Journal of Chinese Medicine v.16 The screening of Chinese traditional drugs by biological assay and the isolation of some active components Han, G.Q.
  9. Korean J. Plant. Res. v.16 no.1 Variation of RAPD patterns between male and female genomic DNAs in dioecious Rumex acetosa L Jin, D.C.;Koo, D.H.;Hur, Y.K.;Bang, J.W.
  10. Crop Sci. v.39 Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean Jung, G.;Skroch, P.W.;Nienhuis, J.;Coyne, P.D.;Arnaud-Santana, E.;Ariyarathnc, H.M.;Marita, J.M.
  11. Theor. Appl. Genet. v.101 Molecular phylogeny of Gossypium species by DNA fingerprinting Khan, S.A.;Hussain, D.;Askari, E.;Stewart, J.Mc.D.;Malik, K.A.;Zafar, Y.
  12. Theor Appl Genet. v.83 Isolation of molecular markers for tomato (L. esculentum) usig random amplified polymorphic DNA (RAPD) Klein-Lankhorst, R.M.;Vermunt, A.;Weide, R.;Liharska, T.;Zabel, P.
  13. Theor. Appl. Genet. v.90 RAPD fingerprinting of blackurrant cultivars (Ribes nigrum L.) cultivars Lanham, P.G.;Brennan, R.M.;Hackett, C.;McNicol, R.J.
  14. Korean J. Medicinal Crop Sci. v.8 no.3 Discrimination of the three Angelica species using the RAPDs and Internal Root Structure Lee, M.Y.;Im, S.H.;Ju, Y.S.;Han, K.S.;Jeong, G.J.;An, D.G.;Kang, H.C.;Ko, B.S.
  15. Nucleic Acids Research v.32 no.4 A novel method for screening species-specific gDNA probes for species identification Li, T.X.;Wang, J.K.;Bai, Y.F.;Sun, X.D.;Lu, Z.H.
  16. Theor. Appl. Genet. v.98 Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.) Mandolino, G.;Carboni, A.;Forapani, S.;Faeti, V.;Ramalli, P.
  17. proc Natl. Acad Sci. USA. v.88 Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenetic lines Martin, G.B.;Williams, J.G.K.;Tanksley, S.D.
  18. Proc. Natl. Acad Sci. USA v.88 Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations Michelmore, R.W.;Paran, I.;Kesseli, R.V.
  19. Sci. Hort. v.62 The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.) Moreno, J.R.;Gogorcena, Y.;Ortiz, J.M.
  20. Genome v.34 Identification of RFLP and RAPD markers linked to downy mildew resistance genes in lettuce by using near-isogenic lines Paran, I.;Kesseli, R.V.;Michelmore, R.W.
  21. Annals of Botany v.91 Genetic Relationships among Orobanche Species as Revealed by RAPD Analysis Roman, B.;Alfaro, C.;Torres, A.M.;Moreno, M.T.;Satovic, Z.;Pujadas, A.;Ruiales, D.
  22. Korean J. Medicinal Crop Sci. v.12 no.1 Discrimination of Angelica Radix Based on anatomical characters Sung, J.S.;Bang, K.H.;Park, C.H.;Park, C.G.;Yu, H.S.;Park, H.W.;Seng, N.S.
  23. Fitoterapia. v.56 Chemical and clinical evaluation of crude drugs derived from Angelica acutiloba and A. sinensis Terasawa, K.
  24. Nucleic Acids Research v.18 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.