Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Precursor and Thidiazuron on Triterpene Saponin Biosynthesis in Centella asiatica (L.) Urban

  • Kim, Ok-Tae (Dept. of Biology & Institute of Plant Resources, Chonnam Natl. Univ.) ;
  • Kim, Min-Young (Dept. of Biology & Institute of Plant Resources, Chonnam Natl. Univ.) ;
  • Ahn, Jun-Cheul (Dept. of Life Sciences, Seonam Univ.) ;
  • Li, Mei-Yang (Dept. of Biology & Institute of Plant Resources, Chonnam Natl. Univ.) ;
  • Hwang, Baik (Dept. of Biology & Institute of Plant Resources, Chonnam Natl. Univ.)
  • Published : 2005.04.30
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Abstract

Plants have been known to accumulate a very diverse range of triterpene saponins. We have investigated the regulation of saponin biosynthesis in higher plants using Centella asiatica (L.) Urban as a model plant. Effects of a feeding precursor on asiaticoside production from leaves and on the level of two-type OSCs mRNA were investigated. As a feeding precursor, squalene negatively affected the levels of CYS and bAS mRNA, but it also decreased the production of asiaticoside from whole plants. Plant hormones regulate secondary metabolism, and in plant tissue cultures they could affect both culture growth and secondary metabolite production. Although enhancement of asiaticoside production from whole plant cultures by addition of TDZ (thidiazuron) has been reported, the positive effect of TDZ on the levels of OSCs transcripts was not observed.

Keywords

References

  1. Chem. Rev. v.93 Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes Abe, I.;Rohmer, M.;Prestwich, G.D.
  2. Prog. Lipid Res. v.36 Cloning of cDNAs or genes encoding enzymes of sterol biosynthesis from plants and other eukaryotes: Heterologous expression and complementation analysis of mutations for functional characterization Bach, T.J.;Benveniste, P.
  3. Plant Cell Tiss. Org. Cult. v.60 Influence of feeding precursors on tropane alkaloid production during an abiotic stress in Datura innoxia transformed roots Boitel-Conti, M.;Laberche, J.C.;Lanoue, A.;Ducrocq, C.;Sangwan-Norreel, B.A.
  4. Science v.267 Host range of a plant pathogenic determined by a saponin detoxifiying enzyme Bowyer, P.;Clarke, B.;Lunness, P.;Daniels, M.;Osbourn, A.
  5. Plant Cell Tiss. Org. Cult. v.56 Effect of alkaloid precursor feeding and elicitation on the accumulation of secologanin in a Catharanthus roseus cell suspension culture Contin, A.;van der Heijden, R.;Verpoorte, R.
  6. Proc. Natl. Acad. Sci. USA v.90 Isolation of Arabidopsis thaliana encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen Corey, E.J.;Matsuda, S.P.T.;Bartel, B.
  7. Influence of culture conditions on secondary metabolite accumulation;Plant cell biotechnology Endress, R.
  8. Plant Cell Tiss. Org. Cult. v.68 Effect of phytohormones on growth and alkaloid accumulation by a Catharanthus roseuscell suspension cultures fed with alkaloid precursors tryptamine and loganin El-Sayed, M.;Verpoorte, R.
  9. Exp. Cell Res. v.50 Nutrient requirements of suspension culture of soybean root cells Gamborg, O.L.;Miller, R.A.;Ojima, K.
  10. Saponins Hostettmann, K.;Marston, A.
  11. Centella;Hager's Handbuch der Pharmazeutischen Praxis. Band 4 Kartnig, T.;Hoffmann-Bohm, K.;Hansel, R.(ed.);Keller, K.(ed.);Rimpler, H.(ed.);Schneider(ed.)
  12. J Plant BioI. v.47 Effect of growth regulators on asiaticoside production in whole plant cultures of Centella asiatica (L.) Urban Kim, O.T.;Kim, M.Y.;Huh, S.M.;Ahn, J.C.;Seong, N.S.;Hwang, B.
  13. Plant Cell Rep. v.23 Stimulation of asiaticoside production from Centella asiatica (L.) Urban whole plant cultures by elicitors Kim, O.T.;Kim, M.Y.;Hong, M.H.;Ahn, J.C.;Hwang, B.
  14. Urban. Mol. Cells Cloning of expression of farnesyl diphosphate synthase cDNA from Centella asiatica (L.) Kim, O.T.;Ahn, J.C.;Hwang, S.J.;Hwang, B.
  15. Plant Cell Rep. Cloning of a cDNA probably encoding oxidosqualene cyclase associated with asiaticoside biosynthesis from Centella asiatica (L.) Urban Kim, O.T.;Kim, M.Y.;Huh, S.M.;Bai, D.G.;Ahn, J.C.;Hwang, B.
  16. Biotechnol. Bioprocess Eng. v.10 Cloning and molecular analysis of cDNA encoding cycloartenol synthase from Centella asiatica (L) Urban Kim, O.T.;Kim, M.Y.;Ahn, J.C.;Hwang, S.J.;Hwang, B.
  17. Chem. Pharm. Bull. v.49 Medicinal Foodstuffs. XXVII. Saponin constituents Gotu Kola (2): Structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponin B, C, and D, from Centella asiaticacultivated in Sri Lanka Matsuda, H.;Morikawa, T.;Ueda, H.;Yoshikawa, M.
  18. Physiol. Mole. Plant Pathol. v.45 An oat species lacking avenacinis susceptible to infection by Gaeumannomyces graminis var. tritici Osbourn, A.;Clarke, B.;Lunness, P.;Scott, P.;Daniels, M.J.
  19. Molecular cloning: a laboratory manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.