Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effects of Naoxintong-containing serum on NO and CGRP in rat cerebral microvascular endothelial cells

  • Lanfang, Li (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Canghai, Li (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Haixia, Dang (Shaanxi Xianyan Changfeng Pharmaceutics Company) ;
  • Nan, Jiang (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Jianyou, Guo (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Shuying, Guo (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Hairu, Huo (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM) ;
  • Tingliang, Jiang (Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of TCM)
  • Published : 2005.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of Naoxintong (NXT, a formula of Chinese Materia Medica)-containing serum on Nitrogen monoxide (NO) and calcitonin gene related peptide (CGRP) in rat cerebral microvascular endothelial cells (rCMEC) was investigated, rCMEC was injured in vitro by incubating for 4 hours at 100% NO in a hypoxia chamber. The results indicated that NXT could antagonize the reduction of NO and CGRP secreted by rCMEC during hypoxia, the effect of which was dose-dependent. After treated with NXT-containing serum at dosage of 5.0 - 30 and 50 -1.1 g/kg/U respectively, the amount of NO and CGRP secreted by rCMEC were remarkably increased during hypoxia in vitro.

Keywords

References

  1. Gordon EL, Danielsson PE, Nguyen TS. (1991) A comparison of primary cultures of rat cerebral microvascular endothelial cells to rat aortic endothelial cells. In Vitro Cell. Dev. Biol. 27, 312-326. https://doi.org/10.1007/BF02630909
  2. Han GM. (2001) The significance of assay the ET, CGRP on illness of cerebral vasculars in clinical. J. Radioimmunol. 14, 141.
  3. Hirate Y. (2003) Nitro oxide in hypertension. J. Clin. Exp. Med. 204, 607-610.
  4. Li Q. (2000) Eu Chang NXT Treating 31 cases of vascular dementia. Lishizhen Med. Mater. Med. Res. 11, 535-536.
  5. Wang HQ, Liu LQ, Sun HY. (2001) Clinical observation of Eu Chang NXT in treating ischmic cerebrovascular disease. Mod. J. Int. Chinese Trad. West. Med. 10, 720.
  6. Xu YG, Liu Y. (1997) Culture and morpholocical observation of rat cerebral microvascular endothelial cell. J. Micro. Tech. 63-65.
  7. Yin CH, Li LF, Jin YH, Yun GD, Fu YJ, Jiang TL. (2000) Inhibitive effect of DGBXT-containing serum of mouse with hepatic injury on production of hepatic LPO. Chinese J. Exp. Trad. Med. Formulae 6, 32-34.