THE EFFECTS OF INSULIN-LIKE GROWTH FACTOR I (IGF-I) ON EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) MRNA IN MG-63 OSTEOBLASTLIKE CELLS

MG-63 세포주에서 Vascular Endothelial Growth Factor (VEGF) mRNA 발현에 대한 Insulin-like Growth Factor I (IGF-I)의 효과에 대한 연구

  • Suh, Je-Duck (Department of Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital) ;
  • Myung, Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry) ;
  • Kang, Nara (Department of Oral and Maxillofacial Surgery, Ewha Womans University, College of Medicine) ;
  • Choung, Pill-Hoon (Department of Oral and Maxillofacial Surgery, Seoul National University, College of Dentistry)
  • 서제덕 (서울대학교 보라매병원 치과 구강악안면외과) ;
  • 명훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 강나라 (이화여자대학교 의과대학 구강악안면외과) ;
  • 정필훈 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.10.30

Abstract

Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea $(76.1\;{\mu}g/ml)$, actinomycin D $(2.5\;{\mu}g/ml)$, cycloheximide $(10\;{\mu}g/ml)$ were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time-and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.

Keywords

References

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