Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제31권6호
  • /
  • Pages.515-525
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  • 2005
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

백서 편측 설신경 손상 후 미각 및 설유두의 변화에 대한 연구

CHANGE OF TASTE PREFERENCE AND TASTE BUD AFTER UNILATERAL LINGUAL NERVE TRANSECTION IN RAT

  • 김윤태 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 전승호 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 염학렬 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 강진한 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 안강민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김성민 (강릉대학교 치과대학 구강악안면외과학교실) ;
  • 장정원 (연세대학교 의과대학 약리학교실) ;
  • 박경표 (서울대학교 치과대학 구강생리학교실) ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실)
  • Kim, Yoon-Tae (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Jeon, Seung-Ho (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Yeom, Hak-Ryol (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kang, Jin-Han (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Ahn, Kang-Min (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Sung-Min (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University) ;
  • Jahng, Jeong-Won (Dept. of Pharmacology, BK21 for Medical Science, College of Medicine, Yonsei University) ;
  • Park, Kyung-Pyo (Dept of Oral Physiology, College of Dentistry, Seoul National University) ;
  • Lee, Jong-Ho (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 발행 : 2005.12.31
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초록

Purpose of study: Lingual nerve damage can be caused by surgery or trauma such as physical irriatation, radiation, chemotherapy, infection and viral infection. Once nerve damage occurred, patients sometimes complain taste change and loss of taste along with serious disturbance of tongue. The purpose of this study was to evaluate the effects of unilateral lingual nerve transection on taste as well as on the maintenance of taste buds. Materials & Methods: Male Sprague-Dawley rats weighing 220-250g received unilateral transection of lingual nerve, subjected to the preference test for various taste solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) with two bottle test paradigm at 2, 4, 6, or 8 weeks after the operation. Tongue was fixed with 8% paraformaldehyde. After fixation, they were observed with scanning electron microscope(JSM-$840A^{(R)}$, JEOL, JAPAN) and counted the number of the dorsal surface of the fungiform papilla for changes of fungiform papilla. And, Fungiform papilla were obtained from coronal sections of the anterior tongue(cryosection). After cryosection, immunostaining with $G{\alpha}gust$(I-20)(Santa Cruz Biotechnology, USA), $PLC{\beta}2$(Q-15)(Santa Cruz Biotechnology, USA), and $T_1R_1$(Alpha Diagnostic International, USA) were done. Immunofluorescence of labeled taste bud cells was examined by confocal microscopy(F92-$300^{(R)}$, Olympus, JAPAN). Results: The preference score for salty and sweet tended to be higher in the operated rats with statistical significance, compared to the sham rats. Fungiform papilla counting were decreased after lingual nerve transaction. In 2 weeks, maximum differences occurred. Gustducin and $T_1R_1$ expressions of taste receptor in 2 and 4 weeks were decreased. $PLC{\beta}2$ were not expressed in both experimental and control group. Conclusion: This study demonstrated that the taste recognition for sweet and salty taste changed by week 2 and 4 after unilateral lingual nerve transection. However, regeneration related taste was occurred in the presence of preserving mesoneurial tissue and the time was 6 weeks. Our results demonstrated that unilateral lingual nerve damage caused morphological and numerical change of fungiform papilla. It should be noted in our study that lingual nerve transection resulted in not only morphological and numerical change but also functional change of fungiform papillae.

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