Immunomodulatory Activities of Oat Bran Extracts with Different Extraction Conditions

추출조건에 따른 귀리 추출물의 면역활성

  • Park, Hee-Joeng (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Yun-Bae (College of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Tae-Su (Department of Food Science and Biotechnology, Chungbuk Provincial University) ;
  • Jung, Ick-Soo (Technical Research Institute, Bolak Company Limited) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2005.02.28

Abstract

Immunomodulatory activities of crude ${\beta}$-glucans extracted from oat bran under different conditions, fractions A ($55^{\circ}C,\;5%,\;pH\;6$), B ($45^{\circ}C,\;15%,\;pH\;6$), C ($50^{\circ}C,\;20%,\;pH\;7$), D ($50^{\circ}C,\;0%,\;pH\;7$), and E ($50^{\circ}C,\;10%,\;pH\;9$) were investigated. All crude ${\beta}$-glucan fractions stimulated macrophages, producing nitric oxide dose-dependently, and, efficiently promoted nitric oxide production in presence of IFN-${\gamma}$. Except for fraction C, in vivo test indicated fractions B, D, and E (100 mg/kg) substantially enhanced carbon-phagocytic indices of blood macrophages by oral administration of crude ${\beta}$glucan for 7 days prior to carbon injection. These immunomodulatory effects could be determined with extraction conditions of crude ${\beta}$-glucan.

추출조건에 따른 귀리의 crude ${\beta}$-glucan에 대한 면역증진효과을 살펴보기 위해 추출온도, 에탄올 농도 및 pH를 달리하면서 얻은 ${\beta}$-glucan 분획 중 in vitro 실험에서 항암성이 우수하게 나타난 5개 분획인 A분획 ($55^{\circ}C,\;5%,\;pH\;6$) B분획($45^{\circ}C,\;15%,\;pH\;6$), C분획 ($50^{\circ}C,\;20%,\;pH\;7$), D분획 ($50^{\circ}C,\;0%,\;pH\;7$) 및 E 분획($50^{\circ}C,\;10%,\;pH\;9$)를 대상으로 복강 큰 포식세포의 nitric oxide 생성능과 경구투여 후 혈액중의 carbon 탐식효율을 조사하였다. In vitro 실험에서 ${\beta}$-glucan을 $10{\sim}1,000{\mu}g/mL$ 범위에서 단독으로 또는 $IFN-{\gamma}$와 병행 처리했을 때 모두 대식 세포의 nitric oxide 생성량은 처리 농도에 비례하여 증가하였다. 단독 처리시에는 고용량일 때($1,000{\mu}g/mL$) 모든 분획에서 nitric oxide 생성량이 유의적으로 증가하였다. $IFN-{\gamma}$와 병행 처리시에는 C분획을 제외한 모든 분획의 고농도 처리구에서 유의적으로 증가하였는데, 특히 E분획은 $100{\mu}g/mL$의 낮은 농도에서도 효율적인 증가를 보였다. In vivo 실험에서 100mg/kg의 ${\beta}$-glucan 분획을 7일간 경구투여한 후 carbon 탐식효율을 조사한 결과 B, D 및 E 분획에서 유의적인 효과를 나타내었다. 이상의 결과를 종합해볼 때, ${\beta}$-glucan은 고용량일 때 직접적으로 또는 $IFN-{\gamma}$ 존재시에는 저용량에서도 복강 큰 포식세로를 활성화시킬 뿐 아니라, 탐식효율도 높임으로써 면역기능을 증진 시키는 것으로 나타났고, 그 효과는 crude ${\beta}$-glucan의 추출조건에 따라 달라지는 것을 알 수 있었다.

Keywords

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