Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Antioxidative and Protective Effects of Ulmus davidiana var. japonica Extracts on Glutamate-Induced Cytotoxicity in PC 12 Cells

느릅나무 추출물의 항산화 효과 및 L-glutamate 유래 PC12 세포독성 보호효과

  • Choi, Won-Hee (Department of Food Function Research Division, Korea Food Research Institute) ;
  • Oh, Young-Sang (Department of Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Sung-Ran (Department of Food Function Research Division, Korea Food Research Institute) ;
  • Ahn, Ji-Yoon (Department of Food Function Research Division, Korea Food Research Institute) ;
  • Ha, Tae-Youl (Department of Food Function Research Division, Korea Food Research Institute)
  • Published : 2005.06.30
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Abstract

Antioxidative and protective effects of Ulmus davidiana var. japonica against oxidative damages induced by glutamate in PC 12 cells were investigated. Inhibitory activity against $FeSO_{4}-H_{2}O_{2}$-induced oxidative stress and DPPH radical-scavenging activity were detected in ethyl acetate and butanol fractions of ethanol extracts from stems and roots. Ethyl acetate and butanol fractions of ethanol extracts from roots significantly inhibited glutamate-induced cytotoxicity and reactive oxygen species in PC 12 cells. These results demonstrate ethyl acetate and butanol fractions of ethanol extracts of U. davidiana var. japonica have potent protective effect against glutamate-induced oxidative stress.

느릅나무 에탄올 추출물의 각 용매 분획물에 대한 항산화활성과 L-glutamate에 의하여 유도된 PC 12세포에 대한 세포 독성 및 세포 내 활성산소종(ROS)을 측정하였다. 느릅나무 수피의 에틸아세테이트 및 부탄올 분획과 근피의 에틸아세테이트 분획층은 흰쥐 뇌조직에서 $FeSO_{4}-H_{2}O_{2}$로 유도한 산화적 스트레스를 유의하게 억제하였으며 강한 fire radical 소거능을 나타내었다. 또한 glutamate에 의하여 PC 12 세포의 생존율이 억제되었고 세포내 ROS가 증가하였으며 이러한 ROS의 증가는 근피의 에틸아세테이트 및 부탄올 획분에 의하여 억제되었다. 따라서 느릅나무 근피의 에틸아세테이트 및 부탄올 획분은 PC 12 세포에서 glutamate로 유도된 세포내 ROS를 억제함으로써 세포의 독성을 보호한 것으로 사료되었다.

Keywords

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