Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Cholesterol-Lowering Effect of Pine Nut in Plasma of Rats

잣 성분의 혈중 콜레스테롤 저하효과

  • Park, Young-Seo (Division of Biotechnology, Kyungwon University) ;
  • Chung, Myong-Soo (Department of Packaging, Yonsei University, Institute of Functional Biomaterials and Biotechnology, Yonsei University)
  • 박영서 (경원대학교 생명공학부) ;
  • 정명수 (연세대학교 패키징학과, 연세대학교 생리활성소재연구소)
  • Published : 2005.10.31
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Abstract

Cholesterol-lowering effect of pine nut on mice was investigated by examining changes in body weight, feed intake, and triglyceride, total cholesterol, high density liporotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and very low density lipoprotein cholesterol (VLDL-C) contents in mice plasma. Changes in body weight and feed intake between pre-designed experimental animal groups fed various contents of cholesterol and pine nut and control group fed basal diet were not significantly different. Food efficiency ratio of experimental groups fed higher than 10% pine nut was significantly higher than that of control group. Apparent differences in atherogenic index representing cholesterol-lowering effect in plasma were not found among all groups fed 0-20% pine nut. Inhibition activities of water and hexane extracts of pine nut on HMG-CoA reductase and cholesterol esterase were examined in vitro. Hexane extract showed 66% inhibition effect on HMG-CoA reductase, whereas none was observed with water extract.

잣의 혈중 콜레스테롤 저하효과를 동물실험을 통하여 확인하기 위하여 기본식이(정상군), 기본식이에 콜레스테롤을 첨가한 식이(대조군), 그리고 기본식이에 콜레스테롤과 여러 농도의 잣을 함유한 실험식이(실험군)를 각 군별로 섭취하게 한 결과 잣의 농도에 따른 식이섭취량과 체중변화량의 유의적인 차이는 크게 나타나지 않았다. 식이효율은 저농도로 잣을 섭취한 경우에는 대조군과 차이를 나타내지 않았지만 10% 이상의 고농도의 잣을 섭취한 경우에는 식이효율이 높았다. 본 연구에 사용한 농노범위(1-20%)에서는 잣 섭취에 의해 혈중 콜레스테롤 함량과 동맥경화지수가 감소하는 효과가 나타나지 않았으며 이는 잣에 함유된 다량의 지방이 섭취됨으로써 잣이 지니는 콜레스테롤 저하효과가 감쇄하였기 때문으로 사료된다. 또한 잣 성분이 HMG-CoA reductase와 cholesterol esterase의 저해활성을 지니는지 확인하기 위하여 hexane과 물을 사용하여 잣 성분을 추출한 후 각 추출물에 대한 HMG-CoA reductase와 cholesterol esterase의 저해활성을 측정한 결과 잣의 hexane 추출물은 66%의 HMG-CoA reductase 저해활성을 지니고 있었으며 물 추출물은 저해활성을 나타내지 않았다. 한편 잣의 hexane 추출물과 물 추출물은 cholesterol esterase에 대한 저해활성은 지니고 있지 않았다. 결론적으로 혈중 콜레스테롤의 함량 저하효과는 가공하지 않은 잣의 섭취에 의해서는 기대하기 어려우므로, 향후 잣의 hexane 추출물 중의 유용성분이 혈중 콜레스테롤 함량 저하효과를 지니는지에 대한 동물실험을 수행할 가치가 있다고 판단된다.

Keywords

References

  1. Glueck CJ, Connor WE. Diet-coronary heat disease relationships reconnoitered. Am. Clin. Nutr. 31: 727-737 (1978) https://doi.org/10.1093/ajcn/31.5.727
  2. Rahimtoola, SH. Cholesterol and coronary heart disease.: A perspective. J. Am. Med, Assoc. 253: 2094-2095 (1985) https://doi.org/10.1001/jama.253.14.2094
  3. Castelli WP, Wilson PW, Levy D, Anderson K. Serum lipid and risk of coronary artery disease. Atherosc. Rev. 21: 7-15 (1990)
  4. Shls ME, Young V. Modern Nutrition in Health and Disease. 7th ed, Lea & Febiger PA, USA (1988)
  5. Vergroeson AT. Physiological effects of dietary linoleic acid. Nutr. Rev. 35: 1-5 (1997)
  6. Bidlack WR, Tappel AL. Damage to microbial membrane by lipid peroxidation. Lipids 8: 177-185 (1973) https://doi.org/10.1007/BF02544631
  7. Saito M. International between lipid peroxide formation and nutritional status. J. Jpn. Soc. Nutr. Food Sci. 41: 343-351 (1988) https://doi.org/10.4327/jsnfs.41.343
  8. Keys A. Coronary heart disease in seven countries. Circulation 41 (suppl. 1): 1-19 (1970) https://doi.org/10.1161/01.CIR.41.1.1
  9. Grover SA, Coupal L, Hu XP. Identifying adults at increased risk of coronary disease. J. Am. Med. Assoc. 274: 801-806 (1995) https://doi.org/10.1001/jama.274.10.801
  10. Connelly PW, MacLean DR, Horlick L, O'connor B, Petrasovits A, Littel JA. Plasma lipids and lipoproteins and the prevalence of risk for coronary heart disease in Canadian adults. CMAJ. 146: 1977-1987 (1992)
  11. Anonymous. Report of the national cholesterol education program expert panel on detection, evaluation, and treatment of high blood cholesterol in adults. Arch. Intern. Med. 148: 36-39 (1988) https://doi.org/10.1001/archinte.148.1.36
  12. Frick MH, Elo O, Haapa K, Heinonen OP, Heinsalmi P, Helo P, Huttunen JK, Kaitaniemi P, Koskinen P, Manninen V. Helsinki Heart Study: Primary-prevention trial with gerfibrozil in middleaged men with dyslipidemia. Safety of treatment, changes in risk factors, and incidence of coronary heart disease. New Engl. J, Med. 317: 1237-1245 (1987) https://doi.org/10.1056/NEJM198711123172001
  13. Sharnir R, Johnson WJ, Zolfaghari R, Lee HS, Fisher EA. Role of bile salt-dependent cholesterol by intestinal cells. Biochemistry 34: 6351-6358 (1995) https://doi.org/10.1021/bi00019a013
  14. Huang Y, Hui DY. Metabolic fate of pancreas-derived cholesterol esterase in intestine: an in vitro study using Caco-2 cells. J. Lipid Res. 31: 2029-2037 (1990)
  15. Gallo LL, Newbill T, Hyun J, Vahouny GV, Treadwell CR. Role of pancreatic cholesterol esterase in the uptake and esterification of cholesterol by isolated intestinal cells. Proc. Soc. Exp. Biol. Med. 156: 277-281 (1977)
  16. Gallo LL, Wadsworth JA, Vahouny GV, Normal cholesterol absorption in rats deficient in intestinal acyl coenzyme A: cholesterol acyltransferase activity. J. Lipid Res. 28: 381-387 (1987)
  17. Krause BR, Anderson M, Bisgaier EL, Bocan T, Bousley R, Deheat P, Essenburg A, Hamelehle K, Horman R, Kieft K, McNally W, Stanfield R, Newton RS. In vivo evidence that the lipid-regulating activity of the ACAT inhibitor CI-976 in rats is due to inhibition of both intestinal and liver ACAT. J. Lipid Res. 34: 279-294 (1993)
  18. Ogawa K, Terada T, Muranaka Y, Hamakawa T, Ohta S, Okamoto M, Fujii S. Studies on hypolipidernic agents. IV. Syntheses and biological activities of trans- and cis-2-(4-alkylcyclohexyl)-2oxoethyl arenesulfonates. Chern, Pharm. Bull. 35: 3276-3283 (1987) https://doi.org/10.1248/cpb.35.3276
  19. Harris WS, Dujovne CA, Windsor SL, Gerrond LL, Newton FA, Gelfand RA. Inhibiting cholesterol absorption with CP-88,818: Studies in norman and hyperlipidemic subjects. J. Cardiovasc. Pharmacol 30: 55-60 (1997) https://doi.org/10.1097/00005344-199707000-00008
  20. Chun SJ, Park YH. Triglyceride composition of pine nut oil. Korean J. Food Sci. Technol. 16: 179-181 (1984)
  21. Friedwald WT, Levy RI, Fredrickson DS. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502 ( 1972)
  22. Kleinsek DA, Dugan RE, Baker TA, Porter .JW. 3-hydroxy-3methylglutaryl coenzyme A reductase from rat liver. Methods Enzymol. 71: 462-479 (1981) https://doi.org/10.1016/0076-6879(81)71057-7