Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Effect of Pine, Oak, and Lily Pollen Extracts

송화분, 참나무 및 백합화분 추출물의 항산화 효능

  • Kim, Seok-Joong (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Park, Hee-Sung (Department of Biotechnology and Bioindustry, Catholic University of Daegu)
  • 김석중 (대구가톨릭대학교 식품공학과) ;
  • 윤광섭 (대구가톨릭대학교 식품공학과) ;
  • 박희성 (대구가톨릭대학교 생명공학과)
  • Published : 2005.10.31
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Abstract

Antioxidative activities of pine, oak, and lily pollen extracts were evaluated based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability and inhibition of lipid peroxidation in animal tissues. Each pollen was extracted with 50% ethanol, 100% ethanol or water. DPPH radical-scavenging capacity of 50% ethanol extract ($EC_{50}$ 40.0 mg/mL) of pine pollen was higher than those of water (46.8 mg/mL) and 100% ethanol (131.2 mg/mL) extracts of pollen. Fifty percent ethanol (3,2 mg/mL) was also better than 100% ethanol (4.5 mg/mL) and water (8.3 mg/mL) for extraction of oak pollen. For preparation of lily pollen extracts, 100% ethanol was most effective (14.0 mg/mL), followed by water (18.8 mg/mL) and 50% ethanol (24.0 mg/mL). Oak pollen showed higher DPPH radical-scavenging activity than others. Lipid peroxidation in rat brain homogenate induced by ascorbate-Fe3+-EDTA and rat kidney homogenate were inhibited by water extracts of all pollens in dose-dependent manner. Extracts of oak and lily pollen showed higher lipid peroxidation inhibition than pine pollen extract. Polyphenol content was highest in oak pollen extract $(32.5{\pm}0.7\;{\mu}g/mg\;pollen)$, followed by lily extract $(25.9{\pm}1.4\;{\mu}g/mg\;pollen)$ and pine extract $(9.3{\pm}0.7\;{\mu}g/mg\;pollen)$.

송화분, 참나무화분 및 백합화분에 대한 항산화 효능을 DPPH radical 소거능 및 동물조직의 지질산화 억제효능을 이용하여 평가하였다. 각 화분을 ethanol, 50% ethanol 및 물을 이용하여 추출물을 조제한 후 이들에 대한 DPPH radical 소거능을 분석한 결과 50% 소거능을 나타내는 $EC_{50}$ 값은, 송화분의 경우 50% ethanol 추출물(40.0mg/mL)이 가장 낮게 나타났으며 물 추출물(46.8mg/mL), 100% ethanol 추출물(131.2mg/mL) 순 이었다. 참나무화분에서는 50% ethanol 추출물(3.2mg/mL), 100%, ethanol 추출물(4.5mg/mL), 물 추출물(8.3mg/mL) 순이었고 백합화분에서는 100% ethanol 추출물의 $EC_{50}$값이 14.0mg/mL로, 50% ethanol 추출물(24.0mg/mL) 및 물 추출물(18.8mg/mL)에 비해 가장 낮았다. 3 종의 화분에서는 참나무 화분의 DPPH radical 소거능이 우수한 것으로 나타났다. 한편 $ascorbate-Fe^{3+}-EDTA$에 의해 유도되는 뇌조직에서의 지질산화도는 송화분, 참나무화분, 백합화분 추출물에 의해 모두 농도 의존적으로 억제되었으며 신장에서의 지질산화도 억제되었다. 그리고 이 중에서 송화분보다는 참나무와 백합화분의 효능이 우수한 것으로 나타났다. 화분 추출물에 대한 총 polyphenol의 함량을 분석한 결과 참나무화분$(32.5{\pm}2.9{\mu}g/mg\;pollen)$이 백합화분$(25.9{\pm}1.4{\mu}g/mg\;pollen)$이나 송화분$(9.3{\pm}0.7{\mu}g/mg\;pollen)$보다 높게 나타났다.

Keywords

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