The Establishing Test Method of Bactericidal Activity and the Evaluating of Korean Disinfectants/Sanitizers Efficacy

살균소독력 시험법 확립 및 살균소독력 평가

  • Kim, Hyung-Il (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Lee, Kwang-Ho (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Kwak, In-Shin (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Eom, Mi-Ok (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Jeon, Dae-Hoon (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Sung, Jun-Hyun (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Choi, Jung-Mi (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Kang, Han-Saem (Center for Food Safety Evaluation, Korea Food and Drug Administration) ;
  • Kim, Yong-Soo (Korea Health Industry Development Institute) ;
  • Kang, Kil-Jin (Center for Food Safety Evaluation, Korea Food and Drug Administration)
  • 김형일 (식품의약품안전청 식품평가부) ;
  • 이광호 (식품의약품안전청 식품평가부) ;
  • 곽인신 (식품의약품안전청 식품평가부) ;
  • 엄미옥 (식품의약품안전청 식품평가부) ;
  • 전대훈 (식품의약품안전청 식품평가부) ;
  • 성준현 (식품의약품안전청 식품평가부) ;
  • 최정미 (식품의약품안전청 식품평가부) ;
  • 강한샘 (식품의약품안전청 식품평가부) ;
  • 김용수 (한국보건산업진흥원) ;
  • 강길진 (식품의약품안전청 식품평가부)
  • Published : 2005.10.31

Abstract

Bacterial suspension test was used to establish Standardization Test Method to investigate bactericidal activity of disinfectant/sanitizer product. Using acceptable verification methodology, test substance showing 5 log or higher reduction in viable count against Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 6538, representing Gram-negative and -positive bacteria, respectively, under test conditions for $5\;min{\pm}10\;sec\;at\;20{\pm}1^{\circ}C$ was considered to have sanitizing capability. All disinfectant/sanitizer products tested under manufacturer's recommended in-use condition gave good reduction values against major food-poisoning bacteria. This standardized method was valuable for evaluating efficacy of disinfectants/sanitizers and could be used as Standardization Test Method for assessing bactericidal activity

살균소독제의 살균소독력 평가를 위한 표준시험법을 확립하기 위하여 세균현탁액 시험법(bacterial suspension test)에 대해 연구하였다. 살균소독력은 G(-)인 E. coli ATCC 10536과 G(+)인 S. aureus ATCC 6538에 대하여 $20{\pm}1^{\circ}C$에서 $5min{\pm}10^{\circ}sec$간 반응하였을 때 초기근수를 5 log이상 감소시키고, 또한, 시험검증조건을 만족하는지를 검토하여 시험법을 확립하였다. 유통 중인 살균소독제 제품 36종에 대하여 확립된 시험법으로 살균소독력을 시험한 결과 모든 살균소독제는 제조자가 제시한 사용농도에서 살균소독력을 나타내었으며, 주요 식중독 균에 대한 살균효과도 관찰되었다. 확립된 시험법은 살균소독력을 측정하는 국가표준시험법으로서 살균소독제의 살균소독력 평가에 적합하였다.

Keywords

References

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