Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparison of Colorimetric Methods for the Determination of Flavonoid in Propolis Extract Products

프로폴리스추출물 제품의 플라보노이드 함량분석을 위한 비색법의 비교

  • Kim, Eun-Jeong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Hwa-Jung (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kim, Hye-Jeong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Nam, Hye-Seon (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Mi-Keong (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kim, Hae-Young (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Lee, Jin-Ha (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration) ;
  • Kang, Yun-Sook (Nutrition of Functional Food Headquarters, Korea Food and Drug Administration) ;
  • Lee, Jong-Ok (Food Headquarters, Korea Food and Drug Administration) ;
  • Kim, Hee-Yun (Testing and Analysis Team, Gyeongin Regional Food and Drug Administration)
  • 김은정 (경인지방식품의약품안전청 시험분석팀) ;
  • 이화정 (경인지방식품의약품안전청 시험분석팀) ;
  • 김혜정 (경인지방식품의약품안전청 시험분석팀) ;
  • 남혜선 (경인지방식품의약품안전청 시험분석팀) ;
  • 이미경 (경인지방식품의약품안전청 시험분석팀) ;
  • 김해영 (경인지방식품의약품안전청 시험분석팀) ;
  • 이진하 (경인지방식품의약품안전청 시험분석팀) ;
  • 강윤숙 (식품의약품안전청 영양기능식품본부) ;
  • 이종옥 (식품의약품안전청 식품본부) ;
  • 김희연 (경인지방식품의약품안전청 시험분석팀)
  • Published : 2005.12.31
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Abstract

Quantitative analysis of flavonoids in commercial propolis extract products were compared by three colorimetric methods; aluminum chloride method, dinitrophenylhydrazine method and aluminum nitrate method, Aluminum nitrate method in Korea Health Supplement Food Code was proved to be specific only for flavones and flavonols same as aluminum chloride method, while dinitrophenylhydrazine method was specific for flavanones and dihydroflavonols. Therefore, the sum of flavonoid contents determined by 2,4-Dinitrophenylhydrazine method and aluminum nitrate method may represent the real content of total flavonoids. As for the 25 commercial propolis extract products examined, the contents of flavonoid varied from 2.15% to 9.53% except for one product.

본 연구는 유통 중인 프로폴리스추출물제품의 플라보노이드 함량을 3가지 비색법을 이용하여 비교 검토해본 결과 현행 건강기능식품공전의 질산알루미늄법은 염화알루미늄법과 마찬가지로 flavone류와 flavonol류의 확인이 가능하며 디니트로페닐 하이드라진법은 flavanone류와 dihydroflavonol류의 정량이 가능한 것을 확인하였다. 따라서 질산알루미늄법과 디니트로페닐 하이드라진법에 의한 함량의 합이 실제 플라보노이드 함량에 가까운 것으로 확인하였다. 두 방법의 총합에 의한 프로폴리스 추출물 25개 제품의 플라보노이드 함량을 확인한 결과 24개 제품에서 2.15-9.53%로 다양하게 나타났다.

Keywords

References

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