Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Research for Selecting The Optimized Vitamin C Analysis Method

최적 비타민 C 분석법 선정에 관한 연구

  • 최원선 (매일유업(주) 중앙연구소) ;
  • 김영진 (매일유업(주) 중앙연구소) ;
  • 정진영 (매일유업(주) 중앙연구소) ;
  • 김태진 (매일유업(주) 중앙연구소) ;
  • 정병문 (매일유업(주) 중앙연구소) ;
  • 김응률 (매일유업(주) 중앙연구소) ;
  • 정후길 (매일유업(주) 중앙연구소) ;
  • 전호남 (매일유업(주) 중앙연구소)
  • Published : 2005.12.31
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Abstract

2,4-Dinitrophenylhydrazine (DHP), indophenol, and HPLC methods, which are generally used to analyze vitamin C, were evaluated by comparing changes in vitamin C contents in various samples kept at 10 and $20^{\circ}C$. Amount of total ascorbic acid (TAA) in yoghurt A kept at $10^{\circ}C$, as measured by DNP method, decreased from 26.7mg/100mL for early period to 22.8mg/100mL for final period of storage, whereas, on 3rd and 10th storage days, increased contrary to general results. Using indophenol, TAA measured at 28.6 and 9.5mg/100mL, and 30.5 and 14.6mg/100mL using HPLC far early and final periods, respectively. With indophenol and HPLC, TAA amount showed steady tendency to decrease. In conclusion, HPLC is the most suitable method to analyze vitamin C content, and indophenol method can be also used during early storage stage.

본 연구는 일반적으로 사용되고 있는 vitamin C 분석방법인 2,4-dinitrophenylhydrazine(DNP), indophenol, 그리고 HPLC법을 이용하여 시판중인 다양한 제품을 이용하여 보존조건($10^{\circ}C,\;20^{\circ}C$)에 따른 vitamin C 함량을 세 가지 방법으로 비교 검토함으로서 가장 이상적인 분석방법을 조사하였다. $10^{\circ}C$ 보존시 요쿠르트 A 제품에서의 vitamin C 변화 추세를 살펴보면, DNP법은 초기와 말기 TAA 함량이 각자 26.7과 22.8mg/100mL로 측정되었으나, 저장 3일째와 10일째에 오히려 증가하는 현상을 보였으며, 또만 indophenol법은 초기와 말기에 각각 28.6과 9.5mg/100mL로, HPLC법은 30.5와 14.6mg/100mL로 확인되었다. 그리고 본 실험에서는 indophenol법과 HPLC법은 일정한 감소 추세를 보였다. 따라서, 가장 이상적인 vitamin C 분석 방법은 HPLC법이며, 저장 초기에 간단하게 vitamin C 함량을 측정할 경우에는 indophenol법을 이용하여도 신뢰할 수 있는 결과를 얻을 수 있는 것으로 사료된다.

Keywords

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