Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Physiological Activities of Peel of Jeju-indigenous Citrus sunki Hort. Tanaka

제주자생 진귤(Citrus sunki Hort. Tanaka) 과피의 생리활성

  • Kang, Shin-Hae (Technology Innovation Center for Life Science, Cheju National University) ;
  • Lee, Young-Jae (Department of Verterinary Medicine, Cheju National University) ;
  • Lee, Chang-Hong (Center for Effective Microorganisms) ;
  • Kim, Se-Jae (Technology Innovation Center for Life Science, Cheju National University) ;
  • Lee, Dae-Ho (Department of Medicine, Cheju National University) ;
  • Lee, Young-Ki (Department of Medicine, Cheju National University) ;
  • Park, Deok-Bae (Department of Medicine, Cheju National University)
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of Citrus sunki peel and its fermented product extracts on physiological and functional activities of cellular systems were investigated. Ethanol extract of Citrus sunki peel showed potent ROS-scavenging activity using 2',7'-Dichlorofluorescin diacetate as a fluorescent ROS probe in HepG2 cells. Fermented product of C. sunki peel extract markedly suppressed nitric oxide production in lipopolysaccharide (LPS)-activated RAW264.7 murine macrophage cells. Treatment with fermented product of C. sunki peel extract decreased intracellular protein levels of inducible nitric oxide synthase and cyclooxygenase II stimulated by LPS. High doses of fermented product lend to apoptotic cell death in CHO-IR cells.

제주자생 재래감귤종이 하나인 진귤(Citrus sunki Hort. ex Tanaka)의 과피는 전통적으로 매우 중요한 한약재 성분으로 사용되어 왔으나 그 약리학적 효과에 대해서는 과학적인 분석이 되어 있지 못하다. 본 연구에서는 진귤과피추출물과 과피발효 추출물의 1차 항산화활성을 검색하여 발효 후 추출물이 더욱 효과적인 활성을 가지고 있는 사실을 발견하였고 이를 바탕으로 대식세포인 Raw264.7세포에서 산화질소의 생성, 염증유발 단백질(NOS2, Cox-2)의 수준을 억제할 뿐 아니라 동 세포의 생존능을 개선시키는 결과를 얻었다. 그러나 상피세포유래 세포주인 CHO-IR 세포 및 사람의 간암세포주인 HepG2 세포의 생존능은 반대로 발효후 추출물에 의해 억제되는 것으로 나타났다. 이러한 결과들은 진피의 발효후 추출물이 대식세포의 항염증활성을 증가시키는 반면, 종양세포의 증식을 억제하고 세포사멸을 유도하는 다양한 약리효과를 가지고 있음을 의미한다.

Keywords

References

  1. Kim HY. Distribution, taxonomy, horticultural characters of the local Citrus spp. in Cheju, and the genetic markers among them. PhD thesis, Cheonnam National University, Cheonnam, Korea (1988)
  2. Francis AR, Shetty TK. Modulating effect of plant flavonoids on the mutagenecity of n-methyl-n-nitro-n-nitrosoguanidine. Carcinogenesis 10: 1953-1955 (1989) https://doi.org/10.1093/carcin/10.10.1953
  3. Guengerich FP, Kim DM. In vitro inhibition of dihydropyridine oxidation and aflatoxin B1 activation in human liver microsomes by naringenin and other flavonoids. Carcinogenesis 11: 2275-2279 (1990) https://doi.org/10.1093/carcin/11.12.2275
  4. Guthrie N, Carroll KK. Inhibition of mammary cancer by citrus flavonoids. pp. 227-236. In: Flavonoids in the living system. Manthey JA, Buslig BS (eds), Plenum. New York, NY, USA (1998)
  5. Chung SK, Kim SH, Choi YH, Song EY, Kim SH. Status of citrus fruit production and view of utilization in Cheju. Food Ind. Nutr. 5: 42-52 (2000)
  6. AOAC. Official Methods of AOAC Intl. 15th ed. Method 994. Association of Official Analytical Chemists. Washington, DC, USA (1990)
  7. Kim CJ. Screening of physiologically active substance. Jayou Academy, Seoul, Korea. pp. 325-338 (1996)
  8. Yoo KM, Hwang IK. In vitro effect of Yuza (Citrus junos SIEB ex TANAKA) extracts on proliferation of human prostate cancer cells and antioxidant activity. Korean J. Food Sci. Technol. 36: 339-344 (2004)
  9. Blios BS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199 (1958) https://doi.org/10.1038/181958a0
  10. Kang SH, Song JH, Kang HK, Kim SJ, Lee YK, Park DB. Insulin can block apoptosis by decreasing oxidative stress via phosphatidylinositol 3-kinase- and extracellular signal-regulated protein kinase-dependent signaling pathways in HepG2 cells. Eur J. Endocrinol. 148: 147-155 (2003) https://doi.org/10.1530/eje.0.1480147
  11. Schaus R. Griess' nitrite test in diagnosis of urinary infection. J. Am. Med. Assoc. 161: 528-529 (1956) https://doi.org/10.1001/jama.1956.62970060009009d
  12. Moon SW, Kang SH, Jin YJ, Park JG, Lee YD, Lee YK, Park DB, Kim SJ. Fermentation of Citrus unshiu Marc. and functional characteristics of the fermented products. Korean J. Food Sci. Technol. 36: 669-676 (2004)
  13. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 (1970) https://doi.org/10.1038/227680a0
  14. Ryu MR, Kim EY, Bae IY, Park YK. Contents of naringin, hesperidin, and neohesperidin in premature Korean Citrus fruits. Korean J. Food Sci. Technol. 34: 132-135 (2002)
  15. Baik SO, Bock JY, Chun HJ, Jeong SI, Baek SH, Oh HB, Kim IK. Analysis and quantitative distribution of glycosided flavonoids in Citruses and Korean Chung-pi. Analytical Sci. Technol. 14: 340-348 (2001)
  16. Chen YQ, Zhou YQ, Wang MH. Activation of the RON receptor tyrosine kinase protects murine macrophages from apoptotic death induced by bacterial lipopolysaccharide. J. Leukoc. Biol. 71: 359-366 (2002)
  17. Cha JY, Cho YS. Biofunctional activities of Citrus flavonoids. J. Korean Soc. Agric. Chem. Biotechnol. 44: 122-128 (2001)
  18. Park D, Pandey SK, Maksimova E, Kole S, Bernier M. Akt-dependent antiapoptotic action of insulin is sensitive to farnesyl-transferase inhibitor. Biochemistry 39: 12513-12521 (2000) https://doi.org/10.1021/bi000995y