The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Mechanisms of Apoptosis by Combination with Jeongjihwan and Cisplatin in Human Glioblastoma Cells

정지환과 시스플라틴의 신경교아세포종에 대한 세포고사 기전연구

  • Shin Hak-Soo (Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Sun-Woo (Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Min-Goo (Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yun Jong-Min (Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Sin Sun-Ho (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Byung-Soon (Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 신학수 (원광대학교 한의학전문대학원, 원광대학교 한의과학대학 심계내과학교실) ;
  • 이선우 (원광대학교 한의학전문대학원, 원광대학교 한의과학대학 심계내과학교실) ;
  • 이민구 (원광대학교 한의학전문대학원, 원광대학교 한의과학대학 심계내과학교실) ;
  • 윤종민 (원광대학교 한의학전문대학원, 원광대학교 한의과학대학 심계내과학교실) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원, 원광대학교 한의과학대학 심계내과학교실)
  • Published : 2005.06.01
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Abstract

Objectives: Malignant gliomas are often treated with cisplatin (cis-diamminedichloroplatinum(II), CDDP) and radiation but results remain unsatisfactory. Since malignant glioma displays moderate resistance to conventional therapy, a new treatment modality is needed to improve the outcome of patients with these tumors. The aim of this study was to investigate the effects of the combined use of Jongjihwan(JJH) and cisplatin(CDDP) on cultured malignant glioma cells, A172. Methodss & Results: The combined use of cisplatin and Jeongjihwan had synergistic effects on Al72 cells during 24 hr-incubation, This treatment resulted in a decrease of cell viability, Which was revealed as apoptosis Characterized by activation of caspase-3 protease as well as cleavage of poly ADP-ribose polymerase (PARP) with change of mitochondria membrane potential transition. The expression of members of the Bcl-2 protein family was modulated during co-treatment with Jeongjihwan and cisplatin. Activation of caspase-3 and mitochondrial alterations were central to co-treatment with Jeongjihwan and cisplatin-induced apoptosis. Conclusions: We conclude that co-treatment with Jeongjihwan and cisplatin-induced activation of the mitochondrial pathway enables cell death. Also, we suggest the combined theory of JJH and cisplatin could be a useful method for glioblastoma.

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