Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Bioavailability Assessment of Isoflavones between Soybean and Soybean Sprout in Rat

실험쥐를 통한 콩과 콩나물 Isoflavones의 생체이용성 비교

  • Kim Eun Mi (Korea Food Research Institute) ;
  • Kim Kyung-Jin (School of Life Sciences and Biotechnology, Korea University) ;
  • Choi Jin-Ho (School of Life Sciences and Biotechnology, Korea University) ;
  • Chee Kew Mahn (School of Life Sciences and Biotechnology, Korea University)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Rodent models have been used to study the anticarcinogenic properties of the soy isoflavones, particularly genistein, but there is little information regarding the pharmacokinetics of the absorption and excretion of genistein. In this study, rats were given a single oral dose of genistein (20 mg/kg body wt) or an equivalent dose as Myougjoonamul-kong and Myoungjoonamul soy sprouts. Concentrations of genistein were measured in plasma, urine and feces at intervals up to 48hr after dosing. Maximum peak of plasma genistein concentration is 8 hr after dosing, and its concentration is 13.2, 7.4mol/L in soy and soy sprout-treated rats, respectively. In pure genistein treated rats, maximum peak of plasma genistein concentration is 2hr after dosing (5.7 mol/L). The percentage of dose recovered in urine over 48hr was not different between groups ($21.2\%$ soy treated; $18.2\%$ soy sprout treated; $16.1\%$ pure genistein treated). There were no significant differences between groups in the recovery of genistein in feces ($19.5\%,\;7.5\%\;and\;15.7\%$ of doses, respectively). $6.9\%\;and\;6.07\%$ of the daidzein from the soy and soy sprout treated was recovered in the feces.

쥐에게 콩과 콩나물, 순수한 genistein을 함유되어 있는 genistein함량을 기준으로 동일하게 급여 (20mg/kg) 시켰을 때, 체내에서 흡수가 되는 속도는 soy, soy sprout, pure genistein군의 순이며 일반소화율은 soy sprout, pure genistein, soy군의 순이었다. 이로써 soy의 genistein은 쉽게 흡수되고 배설되며, 또한 전혀 흡수되지 않고 배설분으로 나오는 양도 세 그룹 중 가장 많은 것을 알 수 있었다. 이는 담즙 등으로 재순환되지 않는 양이 다른 그룹보다 많기 때문이라고 추정되었다. 따라서 생리기능상의 목적으로 isoflavone을 섭취하고자 할 때에는 콩보다는 콩나물형태로의 섭취가 타당할 것으로 생각되며 정확한 결과를 위해서도 사람이 섭식하는 상태의 콩과 콩나물의 isoflvones의 흡수율을 조사할 필요성이 있을 것으로 생각되었다.

Keywords

References

  1. Anderson RL, Wolf WJ. Compositional changes in trypsin inhibitors, phytic acid, saponins and isofalvones related to soybean processing. J Nutr 125: 581S-588S, 1995
  2. Coward S, Barnes NC, Setchell KDR, Barnes S. Genistein, daidzein and their glucoside congugates: Antitumor isoflavones in soybean foods from American and Asian diets. J Agric Food Chem 31: 394-396, 1993 https://doi.org/10.1021/jf00116a052
  3. Fotsis Bradbury RB, White DE. Estrogen and related substances in plants. In Harris RS, Marrian GF, 1995
  4. Adlercreutz, H, Harnalainen E, Gorbach S, Goldin B. Dietary phytoestrogens and the menopause in Japan. Lancet 339: 123, 1992
  5. Adlercreutz, H, Honjo H, Higashi A, Fotsis T, Harnalainen E, Hasegawa T, Okada H. Urinary excretion of lignans and isoflavonoid phytoestrogens in Japanese man and woman consuming a traditional Japanese diet. Am J Clin Nutr 54: 1093-1100, 1991
  6. Adlercreutz H, Bannwart C, Wahala K, Mahala T, Brunow G, Hase T, Arosemena PJ, Kellis J, Vickery LE. Inhibition of human aromatase by mammalian lignans and isoflavonoid phytoestrogens. J Steroidiochem Mol Bio 44: 147-153, 1993
  7. Adlercreutz H, Van der Wildt J, Kinzel J, Attalla H, Wahala K, Makela T, Hase T, Fotsis T. Lignan and isoflavonoid conjugates in human urine. J Steroid Biochem Molec Biol 52 (1) : 97-103, 1995 https://doi.org/10.1016/0960-0760(94)00146-D
  8. Wang H, Murphy PA. lsoflavone content in commercial soybean foods. J Agric Food Chem 42: 1666-1673, 1994a https://doi.org/10.1021/jf00044a016
  9. Kim SR, Kim SD. Studies on soybean isoflavones: 1. Content and distribution of isoflavons in Korea soybean cultivar. RDA J of Agric Sci 38: 155-165, 1996
  10. Setchell KDR, Cassidy A. Dietary isoflavones: biological effects and relevance to human health. J Nutr 129: 758S-767S, 1999
  11. Murkies AL, Wilcox G, Davis SR. Phytoestrogen-clinical review. J Clin Endocrinol Metab 83: 297-303, 1998 https://doi.org/10.1210/jc.83.2.297
  12. King RA, Broadbent JL, Head RJ. Absorption and excretion of th soy isoflavone genistein in rat. J Nutr 126: 176-182, 1996
  13. Bingham SA, Atkinson C, Liggins J, Bluck L, Coward A. Phytoestrogens: where are we now? Br J Nutr 79: 393-406,1998 https://doi.org/10.1079/BJN19980068
  14. Yamazaki I. Effect of ipriflavone on the response of uterus and thyroid to estrogen. Life Sci 38: 757-764,1996 https://doi.org/10.1016/0024-3205(86)90591-6
  15. Setchell KDR, Borriello SP, Hulme P, Axelson M. Nonsteroidal estrogens of dietary origen: possible roles in hormone-dependent disease. Am J Clin Nutr 40: 569, 1984
  16. Sipes GI, Gandolfi JA. Biotransfonnation of toxicants. In: General Principles of Toxicology Cassarrett & Doull's Tox. Basic Sci Poisons, 1991
  17. Rowland I, Faughnan M, Hoey L, Wahala K, Wiliamson G, Cassidy A. Bioavailability of phyto-estrogens. British J Nutr 89 (suppl. l) : S45-S58, 2003
  18. Payne RL, Bidner TD, Southern LL, Geaghan JP. Effect of dietary soy isoflavones on growth, carcass traits, and meat quality in growing-fInishing pigs. J Anim Sci 79: 1230-1239,2001
  19. Lundh TJ, Pettersson H, Kiessling K. Demethyation and conjugation of formononetin and daidzein in sheep and cow liver microsomes. J Agric Food Chem 36: 22-25,1988 https://doi.org/10.1021/jf00079a006
  20. Lundh TJ, Pettersson H, Kiessling K. Liquid chromatographic determination of the estrogens daidzein, formononetin, coumesterol and equol in bovine plasma and urine. J AOAC 71: 938-941, 1988
  21. Xu X, Harris KS, Wang HI, Muphy PA, Hendrich S. Bioavailability of soybean isoflavones depends upon gut microflora in women. J Nutr 125: 2307-2315, 1995
  22. Wiseman H. The bioavailability of non-nutrient plant factors: dietary flavonoids and phytoestrogens. Procedings of the Nutri Society 58: 139-146,1999
  23. Watanbe T, Kondo K, Oishi M. Induction of in vitro differentiation of mouse erythroleukemia cells by genistein, an inhibitor of tyrosine prokinase. Cancer Res 51: 764, 1991
  24. Cassidy A, Bingham S, Setchell KDR. Biological effect of a diet of soy protein rich in isoflavones on the menstrual cycle of premenopausal women. Am J Clin Nutr 60: 333-340, 1994
  25. Adams NR. Detection of the effects of phytoestrogens on sheep and cattle. J Anim Sci 73: 1509-1515, 1995