Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Gamma Irradiation on Physiological Activity of Citrus Essential Oil

감마선 조사가 감귤 정유의 생리활성에 미치는 영향

  • Kim, Hyun-Joo (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute) ;
  • Jo, Cheorun (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute) ;
  • Lee, Na-Young (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute) ;
  • Son, Jun-Ho (Dept. of Cosmeceuticals Science, Daegu Hanny University) ;
  • Yook, Hong-Sun (Dept. of Cosmeceuticals Science, Daegu Hanny University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute)
  • 김현주 (한국원자력연구소 방사선연구원 방사선식품생명공학연구팀) ;
  • 조철훈 (한국원자력연구소 방사선연구원 방사선식품생명공학연구팀) ;
  • 이나영 (한국원자력연구소 방사선연구원 방사선식품생명공학연구팀) ;
  • 손준호 (대구한의대 화장품소재공학과) ;
  • 안봉전 (대구한의대 화장품소재공학과) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 변명우 (한국원자력연구소 방사선연구원 방사선식품생명공학연구팀)
  • Published : 2005.07.01
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Abstract

Physiological activity of citrus essential oil (CEO) was determined to examine possible use of the food processing by-product as a functional material for food and cosmetic composition. The effect of gamma irradiation on the change of physiological activity also investigated at 0, 10 and 20 kGy. Limonene contents of CEO was $88.3\pm1.30\%$. Electron donating ability of CEO was $69\%$. Lipid oxidation was retarded by CEO. CEO showed antimicrobial activity against 1 yeast,4 molds and 4 bacteria species tested. More than $80\%$ of inhibition of cancer cell growth was presented by CEO using A549, HT29, HepG2, B16F10 and G361 cells at a 500 ppm level. Irradiation of CEO did not affect any physiological functions. A Salmonella mutagenicity assay indicated that the 20 kGy irradiated CEO did not show any mutagenicity Therefore, CEO, which is a major by-product in citrus processing, could be used as a functional material in various application.

폐자원을 이용한 기능성 신소재 개발 연구를 위해 감귤 음료 가공 중 생성되는 부산물인 감귤 과피를 추출·정제하여 얻어진 감귤정유의 생리활성 효과를 검증하고 감마선에 의한 영향도 확인하였다 감귤 정유의 limonene의 함량은 $88.3\pm1.30\%$로 나타났으며 감마선 조사한 시료에서도 limonene의 함량은 차이가 없는 것으로 나타났다. 감귤 정유의 전자공여능은 약 70$\%$로 나타났으며 돈육에 감귤 정유 첨가 시 지질산패가 억제 되는것을 확인할 수 있었다. 감글 정유에 대한 항균 활성은 paper disc diffusion test 결과를 바탕으로 농도에 따른 생육저해효과를 측정한 결과 yeast는 250 ppm에서, bacteria는 $2,500\~5,000$ ppm에서, mold는 $5,000\~10,000$ ppm에서 생육이 억제되어, yeast에 대한 항균활성이 가장 우수한 것으로 나타났다. MTT assay를 이용한 세포독성실험 결과 대부분의 암세포에서 약 $90\%$ 이상의 억제율을 보였으며 Ames test를 이용한 복귀돌연변이 시험에서는 $200\mu g/plate$이 하일 때 감귤 정유의 돌연변이 원성이 나타나지 않는 것으로 확인되었다. 전체적인 시험 결과 감마선 조사에 의한 생리활성 변화의 차이는 없는 것으로 나타났다. 따라서 식품 및 공중보건제품 소재로서 가공부산물인 귤 정유는 경제적이면서 효과적으로 사용될 수 있으리라 기대된다.

Keywords

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