Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Analysis and Decrease of Cyanogenic Glucosides in Flaxseed

아마인에 함유된 시안배당체의 분석과 저감화

  • Park, Eun-Ryong (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Hong, Jin-Hwan (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Lee, Dong-Ha (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Han, Sang-Bae (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Lee, Kang-Bong (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Park, Jae-Seok (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Chung, Hyung-Wook (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Hong, Kyung-Hyun (Center for Food Standard Evaluation, Korea Food & Drug Administration) ;
  • Kim, Myung-Chul (Center for Food Standard Evaluation, Korea Food & Drug Administration)
  • 박은령 (식품의약품안전청 식품규격평가부) ;
  • 홍진환 (식품의약품안전청 식품규격평가부) ;
  • 이동하 (식품의약품안전청 식품규격평가부) ;
  • 한상배 (식품의약품안전청 식품규격평가부) ;
  • 이강봉 (식품의약품안전청 식품규격평가부) ;
  • 박재석 (식품의약품안전청 식품규격평가부) ;
  • 정형욱 (식품의약품안전청 식품규격평가부) ;
  • 홍경현 (식품의약품안전청 식품규격평가부) ;
  • 김명철 (식품의약품안전청 식품규격평가부)
  • Published : 2005.07.01
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Abstract

Linustatin and neolinustatin in flaxseed are called cyanogenic glucosides which produce acetone, hydrogen cyanide (HCN) and sugar when hydrolyzed. The HCN is the chemical whose toxin is being issued in food safety. Linustatin and neolinustatin were found in flaxseed by 206.5 and 174.2 mg/100 g, respectively using a RI-HPLC and LC/MSn analysis of $80\%$ methanol extraction. Also, HCN (137.38 mg/kg) was found in flaxseed distillate by autohydrolysis and IC analysis. More than 85$\%$ of linustatin, neolinustatin and HCN were removed when flaxseed was heated for more than 2 hours in $200^{\circ}C$. Especially, HCN was decreased to $98\%$ by heating only 30 min in $200^{\circ}C$.

아마인의 linustatin과 neolinustatin은 가수분해되어 acetone, hydrogen cyanide(HCN) 그리고 sugar를 생성하는 diglucoside 시안배당체로써 가수분해물인 HCN의 독성으로 인해 식품의 안전성에 문제가 되고 있다. $80\%$ methanol 추출과 LC/MSn에 의한 정성분석과 RI-LC에 의한 아마인에서 함유된 시안배당체 함량을 조사한 결과, linustatin과 neolinustatin이 각각 평균 206.5 mg/100 g과 174.2 mg/100 g 함유되어 있었다. 또한 autohydrolysis 후 증류액으로부터 IC 분석 에 의 해 HCN 137.38 mg/kg이 가수분해되어 유리됨을 알 수 있었다. 시안배당체의 저감화를 위하여 가열처리를 수행한 결과, 아마인은 $200^{\circ}C$에서 2시간 이상 가열시 $85\%$ 이상의 linustatin과 neolinustatin 그리고 HCN의 제거효과를 보였다. 특히 $200^{\circ}C$에서 30분 가열시 $98\%$의 HCN이 제거됨을 관찰할 수 있었다.

Keywords

References

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