Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Gentisyl Alcohol Inhibits Apoptosis by Suppressing Caspase Activity Induced by Etoposide

  • KIM JINHEE (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM DONGHYUN (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM MEEREE (Department of Food and Nutrition, Chungnam National University) ;
  • KWON HOJEONG (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University) ;
  • OH TAEKWANG (Microbial Genomics and Applications Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE CHOONGHWAN (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.06.01
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Abstract

In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide ($10\;{\mu}g/ml$), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with $IC_{50}$ value of $3.0\;{\mu}g/ml$ after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

Keywords

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