Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Development of an Enzyme Electrode Biosensor for Lactic Acid Bacteria

효소 전극을 이용한 유산균 측정 바이오센서 개발

  • Park T. S. (School of Biosystems Engineering, Seoul National University) ;
  • Cho S. I. (School of Biosystems Engineering, Seoul National University)
  • Published : 2005.08.01
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Abstract

This study was carried out to develop enzyme biosensor for lactic acid bacteria. Lactic acids produced by lactic acid bacteria (LAB) was measured and good correlation $R^2=0.98$ between LAB count and lactic acids concentration was found. Hydrogen ion produced by L-lactate dehydrogenase (L-LDH) was measured by a potentiometer. Glutamic-pyruvic transminase (GPT) was used for eliminating inhibitor in the reaction. Polyacrylamide gel was used for immobilizing matrix of the sensor. The biosensor was tested and showed good feasibility with $R^2=0.99$ on validation.

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References

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