Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Hepatoprotective Effects of Epimedii Herba through the Antioxidation

음양곽의 항산화작용에 의한 간 보호 효과

  • Ha Bae Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Kim Hee Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Sang Hun (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Jae-Hwa (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee Dong-Geun (Department of Bioscience and Biotechnology, Silla University) ;
  • Park Eun Kyung (Department of Bioscience and Biotechnology, Silla University) ;
  • Nam Chun Suk (Department of Bioscience and Biotechnology, Silla University)
  • Published : 2005.08.01
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Abstract

In this study, the effect of Epimedii Herba (EH) on the antioxidative enzymatic activity was investigated. EH (100mg/kg) was intraperitoneally administered into rats for 2 weeks. On the last day, carbon tetrachloride $(50\%\;CCl_4,\;3.3ml/kg,\;i.p.)$ dissolved in olive oil was injected before 12 hours. EH-pread-ministered and $CCl_4-treated$ (EC) group showed higher inhibitory effect in aminotransferase (AST, ALT) activity compared to $CCl_4-treated (CT)$ group. Superoxide dismutase (SOD) and Catalase in EC group were increased compared to those of CT group. The activity of glutathione peroxidase (GPx) was significantly higher than those of CT group compared to EC group. These results suggest that EH has a hepatoprotective effect through scavenging the free radicals induced by $CCl_4$.

음양곽의 항산화 효과를 규명하기 위하여 흰쥐(Sprague-Dawley계)를 사용하여 음양곽 추출물을 2주간 복강내로 투여하고 $CCl_4$에 의한 간독성 경감기전을 혈액 및 간 조직에서의 효소 변동과 간 조직을 병리학적으로 관찰하였다. $CCl_4$의 투여로 AST, ALT, MDA가 증가하였으나 음양곽을 전처리하고 $CCl_4$를 후 투여한 경우에는 AST, ALT, MDA가 감소하였다. $CCl_4$의 투여로 SOD, catalase, GPx가 감소하였으나 음양 곽 전처리 한 후 $CCl_4$를 투여 한 경우에는 SOD, catalase, GPx가 증가하였다. 음양곽은 $CCl_4$로 유도된 free radicals를 scavenging함으로써 간독성을 경감시키는 것으로 사료된다. 위의 생리활성과 항산화계 변화 지표에서 나타난 음양곽의 간기능 보호효과를 형태학적으로 확인하기 위한 조직병리학적 검사결과 음양곽이 세포막의 소기관들을 과산화로부터 보호함으로써 효소활성의 최적구조를 유지시켜 간 손상을 완화시키는 것으로 사료된다.

Keywords

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