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Antioxidant and Anticancer Activities of Extracts from Styela plicata

주름 미더덕(Styela plicata) 추출물의 항산화력 및 항암활성

  • Kim, Jin-Ju (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Jung (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Sun-Hee (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Division of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
  • 김진주 (경남대학교 식품생명공학부) ;
  • 김선정 (경남대학교 식품생명공학부) ;
  • 김선희 (경남대학교 식품생명공학부) ;
  • 박해룡 (경남대학교 식품생명공학부) ;
  • 이승철 (경남대학교 식품생명공학부)
  • Published : 2005.08.01

Abstract

The antioxidant and anticancer activities of Styela plicata extracts were evaluated. When extracts were prepared with fresh Styela plicata (FR), extraction yield was in the order of methanol > ethanol = acetone > water among treated solvents. However, the extraction order was methanol > water > ethanol > acetate in freeze dried Styela plicata (FD). Radical scavenging activity was the highest in acetone extracts $(37.39\%)$ from FR, while in ethanol extracts $(78.40\%)$ from FD. Reducing power of FR was the greatest in methanol extracts (1.076), and that of FD in ethanol extracts (1.360). The acetone extracts from FD showed significant anticancer activity when revealed with human colon cancer cell line HT-29. These results indicated that extraction yields and properties of extracts from Styela plicata were variable depending on solvent and/or physicochemical state, and appropriate extraction process could provide some valuable bioactive materials from Styela plicata.

주름 미더덕(Styela plicata)에 methanol, ethanol, acetone, 물을 가하여 추출물을 제조하여 항산화활성과 항암활성을 조사하였다. 신선 주름 미더덕을 분쇄하여 용매를 가하여 추출물을 제조한 경우 methanol을 이용하였을 때 가장 많이 추출되었으며, 물을 이용한 경우에 가장 추출율이 낮았다. 그러나, 동결건조한 주름 미더덕 분말로부터 추출물을 제조한 경우에는 물을 이용한 경우에서 추출량이 크게 증가하였다. 각 추출물의 라디칼 소거능을 측정 한 결과, 신선 주름 미더덕 추출물의 경우에는 acetone추출물이 가장 높은 활성 $(37.39\%)$을 보였으며, 동결건조 주름 미더덕 분말로부터 추출한 경우에는 ethanol추출물이 $78.40\%$의 높은 활성을 나타내었다. 환원력은 신선 주름 미더덕 의 경우 methanol 추출물이 가장 좋은 활성을 나타내었고, 동결건조한 주름 미더덕 추출물은 ethanol에서 가장 높은 활성을 보였다. 대장암 세포주 HT-29에 대한 주름 미더덕 추출물의 암세포 증식억제 효과는 동결건조 주름 미더덕의 acetone 추출물이 500${\mu}g/mL$의 농도에서 약$20\%$이하의 높은 활성을 보였다. 이상의 실험 결과로 주름 미더덕에 항산화력과 항암력을 가지는 성분이 함유되어 있음을 알 수 있었다

Keywords

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