Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Beverage Using Herbs on the Antimicrobial, Anticancer and Antiallergy Activities

약초 음료의 항균효과, 항암효과 및 알레르기 억제효과 검증

  • Park, Eun-Mi (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Ye, Eun-Ju (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Kim, Soo-Jung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Kim, Sol-Ah (Solawon) ;
  • Bae, Man-jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University)
  • 박은미 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 예은주 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김수정 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김솔아 (솔아원) ;
  • 배만종 (대구한의대학교 한방바이오식품과학과)
  • Published : 2005.08.01
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Abstract

This study was conducted to investigate the effect of beverage (beverage HC and beverage PG) using herbs on antimicrobial activity, proliferation of hepatic cancer cell (Hep3B) lines and sarcoma 180 (S-180) and antiallergy, respectively. Beverage PG showed higher antimicrobial activity than beverage HC against Staphylococcus aureus and Pseudomonas aeruginosa. Beverage HC and PG showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitoy ratio against tumor cells were $66\%\;for\;10\%$ beverage HC, $61\%\;for\;10\%$ beverage PG. In an anti-cancer test using Hep3B cells, beverage PG showed higher anti-proliferating effect than beverage HC. Beverage PG showed growth-inhibitory effect of $69.2\%\;at\;100\%$ beverage PG. Beverage PG inhibited histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80. In conclusion, these results suggest that beverage using herbs have an antimicrobial activity, anti-proliferating effect against Hep3B cell and S-180 tumor and will be beneficial in treatment of allergic reaction.

약초 음료인 HC와 PG의 항균효과, 항암효과 및 항알레르기효과에 대해 다음과 같은 결과를 얻었다. 원판확산법을 통해 관찰한 S. aureus와 P. aeruginosa에 대한 저지 효과는 약초 음료 HC에 비해 약초 음료 PG에서 더 크게 나타났으며 S. aureus보다 P aeruginosa에 대한 항균력이 더 강하게 나타났다. 48시간 동안 약초발효 음료의 농도를 달리하여 S. aureus와 P. aeruginosa에 대한 증식 저해 정도를 관찰한 결과 S. aureus에 관한 증식 저해 정도는 약초 음료 HC군의 경우 $5\%$대에서부터 확실한 균 증식 억제 정도를 나타내었으며, 48시간 경과 후에는 $10\%$이상의 첨가군에서 균 증식 저해능이 더욱 커짐을 볼 수 있었다 S. aureus에 관한 약초 음료 PG의 증식 저해능은 24시간 경과 후 $5\%$ 첨가군에서 부터 확실한 균증식 억제능을 보였다. P. aeruginosa에 대한 증식 저해 정도는 약초 음료 HC군의 경우 24시간 경과 후 $10\%$대에서부터 확실한 균증식 억제를 나타내었으며, 약초음료 PG의 증식 저 해능은 24시간 경과 후 $5\%$ 첨가군에서 부터 확실한 균 증식 억제능을 나타내었다. 약초 음료 HC와 약초 음료 PG의 고형암(S-180) 성장 억제에 대한 실험 결과 대조군에 비해 $5\%$ HC군에서는 $62\%,\;10\%$ HC군에서는 $66\%$의 고형암 억제효과를 나타내었고, $5\%$ PG군에서는 $53\%,\;10\%$ PG군에서 는 $61\%$의 고형암 성장 억제효과를 나타내었다. 암세포 성장 저해효과 실험결과 인간 유래 간암세포인 Hep3B에 대해 약초 음료 HC원액은 $24.6\%$,약초 음료 PG원액은 $69.2\%$의 저해율를 보였다. 약초 음료 HC와 약초 음료PG의 히스타민 유리 억제효과를 측정한 결과compound 48/80을 처리하여 유리된 히스타민량에 비해 약초 음료PG는 $46.4\%$, 약초 음료 HC는 $17.9\%$의 히스타민 분비 억제효과를 나타내었다.

Keywords

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