YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Inhibitory Effect of Polyphenol Oxidase on Polyphenol-Induced DNA Damage of Bacillus subtilis

고초균에서 폴리페놀로 유도된 DNA 손상에 대한 폴리페놀산화효소의 억제효과

  • 김안근 (숙명여자대학교 약학대학) ;
  • 김유경 (숙명여자대학교 약학대학) ;
  • 강영숙 (숙명여자대학교 약학대학)
  • Published : 2005.08.01
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Abstract

Antimutagenic activity of the enzymatic browning reaction products (EBRPs) was investigated by using the spore rec-assay with Bacillus subtilis strains H17 $(rec^+)\;and\;M45 (rec^-)$. The EBRPs tested were prepared from the reactions of five different kinds of polyphenols with polyphenol oxidase isolated from the leaves Perilla frutescens. In the spore rec-assay, most of the polyphenolic compounds tested showed positive, whereas only their tested compound showed negative respectively. In addition of polyphenol oxidase inhibitors such as cysteine, glutathione and ascorbic acid to the reaction mixtures consisted with the polyphenol oxidase and polyphenols, the mutagenic effects were increased in the spore recassay. These results show that the activity of polyphenol oxidase may play an important role in the reduction of mutagenicity of polyphenols.

Keywords

References

  1. Hind, G., Marshak, D. R. and Coughlan, S. J. : Spinach thylakoid polyphenol oxidase: Cloning, characterization and relation to a putative protein kinase. Biochemistry 34, 8157 (1995) https://doi.org/10.1021/bi00025a022
  2. Mayer, A. M. : Polyphenol oxidases in plants - recent progress. Phytochemistry 26, 11 (1987) https://doi.org/10.1016/S0031-9422(00)81472-7
  3. Ham, S. S., Hong, E. H. and Omura, H. : Desmutagenicity of enzymatically browned substances obtained from the reaction of Prunus salicina (Red) enzyme and polyphenols. Korean J. Food Sci. Technol. 19(3), 212 (1987)
  4. Bailey, J. L. : Technique in Protein Chemistry, Elservier Publisheng Co., New York (1967)
  5. Kada, T., Tutikawa, K. and Sadaie, Y. : In vitro and host mediated 'rec-assay' procedures for screening chemical mutagens. Mutation Res. 16, 165 (1972) https://doi.org/10.1016/0027-5107(72)90177-7
  6. Kada, T., Hirano, K., Hagiwara, T., Ohta, Y. and Matsumoto, T. : rec-Assay with spores of Bacillus subtilis with and without metabolic activation. Mutation Res. 97, 339 (1982) https://doi.org/10.1016/0165-1161(82)90001-2
  7. Sanchez-Ferrer, A., Bru, R. and Garcia-Carmona, F. : Novel procedure for extraction of a latent grape polyphenol oxidase using temperature-induced phase seperation in triton X-114. Plant Physiol. 91, 1481 (1989). https://doi.org/10.1104/pp.91.4.1481
  8. Sanchez-Ferrer, A., Alba, J. V. and Garcia-Carmona, F. : Triton X-114 as a tool for purifying spinach polyphenol oxidase. Phytochemistry 28(5), 1321 (1989) https://doi.org/10.1016/S0031-9422(00)97738-0
  9. Sanchez-Ferrer, A., Bru, R. and Garcia-Carmona, F. : Partial purification of a thylakoid-bound enzyme using temperature-induced phase partitioning. Anal. Biochem. 184, 279 (1990) https://doi.org/10.1016/0003-2697(90)90681-X
  10. Sanchez-Ferrer, A., Laveda, F. and Garcia-Carmona, F. : Substrate-dependent activation of latent potato polyphenol oxidase by anionic surfactants. J. Agric. Food Chem. 41, 1583 (1993) https://doi.org/10.1021/jf00034a010
  11. Chazarra, S., Cubanes, J., Escribano, J. and Garcia-Carmona, F. : Partial purification and characterization of latent polyphenol oxidase in iceburg lettuce (Lactuca sativa L.). J. Agric. Food Chem. 44, 984 (1996) https://doi.org/10.1021/jf9501352
  12. Bordier, C. : Phase separation of integral membrane proteins in triton x-114 solution. J. Biol. Chem. 256, 1604 (1981)
  13. Raymond, J., Rakariyatham, N. and Azanza, J. : Purification and some properties of polyphenol oxidase from sunflower seeds. Phytochemistry 34(4), 927 (1993) https://doi.org/10.1016/S0031-9422(00)90689-7
  14. Partinton, J. C. and Bolwell, G. P. : Purification of polyphenol oxidase free of the storage protein patatin from potato tuber. Phytochemistry 42(6), 1499 (1996) https://doi.org/10.1016/0031-9422(96)00185-9
  15. Baik, C. W. and Ham, S. S. : Antimutagenic effects of browning products reacted with polyphenol oxidase extracted from apple by using SOS chromotest. Korean J. Food Sci. Technol. 22(6), 618 (1990)
  16. Ham, S. S., Kim, S. W. and Kim, Y. M. : Studies on antimutagenic effects and gene repair of enzymatic browning reaction products. Korean J. Food Sci. Technol. 22(6), 632 (1990)