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Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA

  • Park, Myung-Soo (Department of Applied Biology, Chungnam National University) ;
  • Seo, Geon-Sik (Department of Industrial Crops, Korea National Agricultural College) ;
  • Bae, Kyung-Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yu, Seung-Hun (Department of Applied Biology, Chungnam National University)
  • Published : 2005.09.01

Abstract

Molecular profIles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCR­RFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed with described morphological species, except that the RFLP group A containing the two unidentified species. A neighbor-joining tree based on ITS sequences well supported RFLP groups observed by RFLP analysis of ITS regions of rDNA. Additionally, the two unidentified species, Trichoderma sp. 1 and 2, which could not be distinguished by PCR­RFLP analysis, were separated in sequence analysis of ITS regions of rDNA.

Keywords

References

  1. Bissett, J. 1984. A revision of the genus Trichoderma. I. Section Longibrachiatum sect. nov. Can. J Bot. 62:924-931 https://doi.org/10.1139/b84-131
  2. Bissett, J. 1991a. A revision of the genus Trichoderma. II. Infrageneric classification. Can. J Bot. 69:2357-2372 https://doi.org/10.1139/b91-297
  3. Bissett, J. 1991b. A revision of the genus Trichoderma. III. Section Pachybasium. Can. J. Bot. 69:2373-2417 https://doi.org/10.1139/b91-298
  4. Bissett, J. 1991c. A revision of the genus Trichoderma. IV. Additional notes on section Longibrachiatum. Can. J. Bot. 69:2418-2420 https://doi.org/10.1139/b91-299
  5. Bulat, S. A., Lubeck, M., Mironenko, N., Jensen, D. F. and Lubeck, P. S. 1998. UP-PCR analysis and ITS1 ribotyping of strains of Trichoderma and Gliocladium. Mycol. Res. 102:933-943 https://doi.org/10.1017/S0953756297005686
  6. Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph.D. thesis, University of Newcastle, Newcastle upon Tyne, UK
  7. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791 https://doi.org/10.2307/2408678
  8. Fujimori, F. and Okuda, T. 1994. Application of the random amplified polymorphic DNA using the polymerase chain reaction for efficient elimination of duplicate strains in microbial screening I Fungi. J. Antibiot. 47: 173-182 https://doi.org/10.7164/antibiotics.47.173
  9. Gams, W. and Meyer, W. 1998. What exactly is Trichoderma harzianum? Mycologia 90:904-915 https://doi.org/10.2307/3761332
  10. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120 https://doi.org/10.1007/BF01731581
  11. Kindermann, J., EI-Ayouti, Y., Samuels, G. J. and Kubicek, C. P. 1998. Phylogeny of the genus Trichoderma based on sequence analysis of the internal transcribed spacer region I of the rDNA cluster. Fungal Genet. Biol. 24:298-309 https://doi.org/10.1006/fgbi.1998.1049
  12. Kuhls, K., Lieckfeldt, E. and Borner, T. 1995. PCR-fingerprinting used for comparison of ex-type strains of Trichoderma species deposited in different culture collections. Microbiol. Res. 150:1-9 https://doi.org/10.1016/S0944-5013(11)80033-9
  13. Kuhls, K., Lieckfeldt, E., Samuels, G. J., Kovacs, W., Meyer, W., Petrini, O., Gams, W., Bomer T., Meyer, W. and Kubicek, C. P. 1996. Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocreajecorina. Proc. Natl. Acad. Sci. USA 93:7755-7760 https://doi.org/10.1073/pnas.93.15.7755
  14. Kuhls, K., Lieckfeldt, E., Samuels, G. J., Borner, T., Meyer, W. and Kubicek, C. P. 1997. Revision of Trichoderma sect. Longibrachiatum including related teleomorphs based on analysis of ribosomal DNA internal transcribed spacer sequences. Mycologia 89:442-460 https://doi.org/10.2307/3761038
  15. Kullnig-Gradinger, C. M., Szakacs, G. and Kubicek, C. P. 2002. Phylogeny and evolution of the genus Trichoderma: a multigene approach. Mycol. Res. 106:757-767 https://doi.org/10.1017/S0953756202006172
  16. Muthumeenakshi, S., Mills, P. R., Brown, A. E. and Seaby, D. A., 1994. Intraspecific molecular variation among Trichoderma harzianum isolates colonising mushroom compost in the British Isles. Microbiology 140:769-777 https://doi.org/10.1099/00221287-140-4-769
  17. Ospina-Giraldo, M. D., Royse, D. M., Thon, J. R., Chen, X. and Romaine, C. P. 1998. Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90:76-81 https://doi.org/10.2307/3761014
  18. Park, M. S., Seo, G. S., Lee, K. H., Bae, K. S. and Yu, S. H. 2005. Morphological and cultural characteristics of Trichoderma spp. associated with green mold of oyster mushroom in Korea. Plant Pathol. J. 21:221-228 https://doi.org/10.5423/PPJ.2005.21.3.221
  19. Rifail, M. A. 1969. A revision of the genus Trichoderma. Mycological Papers 116:1-116
  20. Samuels, G. J., Petrini, O. and Manguin, S. 1994. Morphological and macromolecular charaterization of Hypocrea schweinitzii and its Trichoderma anamorph. Mycologia 86:421-435 https://doi.org/10.2307/3760575
  21. Samuels, G. J., Dodd, S. L., Gams, W., Castleburry, L. A. and Petrini, O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94: 146-170 https://doi.org/10.2307/3761854
  22. Schlick, A., Kuhls, K., Meyer, W., Lieckfeldt, E., Borner, T. and Messner, K. 1994. Fingerprinting reveals gamma-ray induced mutations in fungal DNA: implication for identification of patent strains of Trichoderma harzianum. Curr. Genet. 26:74-78 https://doi.org/10.1007/BF00326307
  23. Stasz, T. E., Nixon, K., Harman, G. E., Weeden, N. F. and Kuter, G. A. 1989. Evalution of phenetic species and phylogenetic relationships in the genus Trichoderma by cladistic analysis of isozyme polymorphism. Mycologia 81:391-403 https://doi.org/10.2307/3760077
  24. Thompson, J. D., Gibson, T. J., Plewniak, E., Jeanmougin, F. and Higgins, D. G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24:4876-4882
  25. Turner, D., Kovacs, W., Kuhls, K., Lieckfeldt, E., Peter, B., Arisan-Atac, I., Strauss, J., Samuels, G. J., Borner, T. and Kubicek, C. P. 1997. Biogeography and phenotypic variation in Trichoderma sect. Longibrachiatum and associated Hypocrea species. Mycol. Res. 101:449-459 https://doi.org/10.1017/S0953756296002845
  26. Vauterin, L. and Vauterin, P. 1992. Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur. Microbiol. 1:37-41
  27. White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A guide to methods and application (ed. M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White), pp. 315-322. Academic Press: San Diego, U.S.A
  28. Zimand, G, Valisky, L., Elad, Y, Chet, I. and Manulis, S. 1994. Use of the RAPD procedure for the identification of Trichoderma strains. Mycol. Res. 98:531-534 https://doi.org/10.1016/S0953-7562(09)80474-7

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