The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Observations of Infection Structures on the Leaves of Cucumber Plants Pre-treated with Arbuscular Mycorrhiza Glomus intraradices after Challenge Inoculation with Colletotrichum orbiculare

  • Lee, Chung-Sun (Department of Plant Resource Science and the Research Institute for Subtropical Agriculture and Biotechnology, Cheju National University) ;
  • Lee, Yun-Jeong (National Institute of Agricultural Science and Technology, RDA) ;
  • Jeun, Yong-Chull (Department of Plant Resource Science and the Research Institute for Subtropical Agriculture and Biotechnology, Cheju National University)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Resistance inductions on the leaves of cucumber plant by an arbuscular mycorrhiza Glomus intraradices were investigated. In addition, the infection structures were observed at the penetration sites on the leaves of plant inoculated with Colletotrichum orbiculare using a fluorescence microscope. The severity of anthracnose disease caused by Colletotrichum orbiculare was significantly decreased on the leaves of cucumber plant colonized with G intraradices compared with those of non-treated control plants. As a positive control, pre-treatment with DL-3-aminobutyric acid (BABA) caused a remarkable reduction of the disease severity on the pathogen-inoculated leaves. There were no significant differences in the frequency of either germination or appressorium formation of the plant pathogen between mycorrhiza colonized and non-treated plants. It was also the same on the BABA pre-treated plants. However, the frequency of callose formation was significantly high on the leaves of G intraradices colonized plants compared to those of non-treated control plants at 5 days after challenge inoculation. On the leaves of BABA treated plants callose formation was not significantly high than those of non-treated, although the disease severity was more strongly suppressed. It was suggested that the resistance induced by colonization with G. intraradices might be related to the enhancement of callose formation at the penetrate sites on the leaves invaded by the pathogen, whereas resistance by BABA did not.

Keywords

References

  1. Azcon-Aguilar, C. and Barea, J. M. 1996. Arbuscular mycorrhizas and biological control of soil-borne plant pathogens-an overview of the mechanisms involved. Mycorrhiza 6:457-464 https://doi.org/10.1007/s005720050147
  2. Blee, K. A. and Anderson, A. J. 2000. Defense responses in plants to arbuscular mycorrhizal fungi. pp. 27-44. In: G K. Podila and D. D. Douds (eds.), Current advance in mycorrhizae research. Minnesota, USA: The American Phytopathological Society
  3. Burleigh, S. H. and Bechmann, I. E. 2002. Plant nutrient transporter regulation in arbuscular Mycorrhizas. Plant Soil 244:247-251 https://doi.org/10.1023/A:1020227232140
  4. Cohen, Y. 1994. Local and systemic control of Phytophthora infestans in tomato plants by DL-3-amino-n-butanoic acids. Phytopathology 84:55-59 https://doi.org/10.1094/Phyto-84-55
  5. Cordier, C., Gianinazzi S. and Gianinazzi-Pearson, V. 1996. Colonization patterns of root tissues by Phytophthora nicotianae var. parasitica related to reduced disease in mycorrhizal tomato. Plant Soil 185:223-232 https://doi.org/10.1007/BF02257527
  6. Cordier, C., Pozo, M. J., Barea, J. M., Gianimazzi, S. and Gianinazzi-Pearson, V. 1998. Cell defense responses associated with localized and systemic resistance to Phytophthora induced in tomato by an arbuscular mycorrhizal fungus. Mol. Plant-Microbe Interact. 11: 1017-1028 https://doi.org/10.1094/MPMI.1998.11.10.1017
  7. Giovannetti, M. and Mosse, B. 1980.An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol. 84:489-500 https://doi.org/10.1111/j.1469-8137.1980.tb04556.x
  8. Hwang, C. S. and Kolattukudy, P. E. 1995. Isolation and characterization of genes expressed uniquely during appressorium formation by Colletotrichum gloeosporioides conidia induced by the host surface wax. Mol. Gen. Genet. 247:282-294 https://doi.org/10.1007/BF00293196
  9. Hwang, K. H., Sunwoo, J. Y, Kim, Y. J. and Kim, B. S. 1997. Accumulation of $\beta$-1 ,3-glucanase and chitinase isoforms, and salicylic acid in the DL-$\beta$-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsid. Physiol. Mol. Plant Pathol. 51:305-322 https://doi.org/10.1006/pmpp.1997.0119
  10. Jeun, Y. C. 2000. Immunolocalization of PR-protein P14 in leaves of tomato plants exhibiting systemic acquired resistance against Phytophthora infestans induced by pretreatment with 3-aminobutyric acid and preinoculation with Tobacco necrosis virus. J. Plant Dis. Protection 107:352-367
  11. Jeun, Y. C., Park, K. S., Kim, C. H., Fowler, W. D. and Kloepper, J. W. 2004. Cytological Observations of Cucumber Plants During Induced Resistance Elicited by Rhizobacteria. Biol. Control 29:34-42 https://doi.org/10.1016/S1049-9644(03)00082-3
  12. Kloepper, J. W., Leong, J., Teintze, M. and Schroth, M. N. 1980. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 286:885-886 https://doi.org/10.1038/286885a0
  13. Knoester, M., Pieterse, C. M. J., Bol, J. F. and Van Loon, L. C. 1999. Systemic resistance in Arabidopsis induced by rhizobacteria requires ethylene-dependent signaling at the site of application. Mol. Plant-Microbe Interact. 12:720-727 https://doi.org/10.1094/MPMI.1999.12.8.720
  14. Koske, R. E. and Gemma, J. N. 1989. A modified procedure for staining roots to detect VA mycorrhizas. Mycol. Res. 92:486-505 https://doi.org/10.1016/S0953-7562(89)80195-9
  15. Kovats, K., Binder, A. and Hohl, H. R. 1991a. Cytology of induced systemic resistance of cucumber to Colletotrichum lagenarium. Planta 183:484-490
  16. Kovats, K., Binder, A. and Hohl, H. R. 1991b. Cytology of induced systemic resistance of tomato to Phytophthora infestans. Planta 183:491-496
  17. Lamb, C. and Dixon, R. A. 1997. The oxidative burstin plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:251-275 https://doi.org/10.1146/annurev.arplant.48.1.251
  18. Lee, Y. H. and Dean, R. A. 1993. cAMP regulates infection structure formation in the plant pathogenic fungus Magnaporthe griesea. Plant Cell 5:693-700 https://doi.org/10.1105/tpc.5.6.693
  19. Pieterse, C. M. J., Van Wees, S. C. M., Hoffland, E. Van Pelt, J. A. and Van Loon, L. C. 1996. Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumiulation and pathogenesis-related gene experssion. Plant Cell 8:1225-1237 https://doi.org/10.1105/tpc.8.8.1225
  20. Pieterse, C. M. J., Van Wees, S. C. M., Van Pelt, J. A. Knoester, M., Laan, R., Gerrits, H., Weisbeek, P. J. and Van Loon, L. C. 1998. A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell 10: 1571-1580 https://doi.org/10.1105/tpc.10.9.1571
  21. Pozo, M. J., Cordier, C, Dumas-Gaudot, E., Gianinazzi, S., Barea, J. M. and Azon-Aguilar, C. 2002. Localized versus systemic effect of arbuscular mycorrhizal fungi on defense responses to Phytophthora infection on tomato plants. J. Exp. Bot. 53:525-534 https://doi.org/10.1093/jexbot/53.368.525
  22. Pozo, M. J., Dumas-Gaudot, E., Slezack, S., Cordier, C. Asselin, A., Gianinazzi, S., Gianinazzi-Pearson, V. Azon-Aguilar, C. and Barea, J. M. 1996. Detection of new chitinase isoforms in arbuscular mycorrhizal tomato roots; possible implications in protection against Phytophthora nicotianae vat parasitica. Agronmy 16:689-697 https://doi.org/10.1051/agro:19961014
  23. Pozo, M. J., Dumas-Gaudot, E. Azcon-Aguilar, C. and Barea, J. M. 1998. Chitosanase and chitinase activities in tomato roots during interactions with arbuscular mycorrhizal fungi or Phytophthora parasitica. Expt. Bot. 49: 1729-1739 https://doi.org/10.1093/jexbot/49.327.1729
  24. Pozo, M. J., Azcon-Aguilar, C., Dumas-Gaudot, E. and Barea, J. M. 1999. $\beta$-1 ,3-glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and/or Phytophthora parasitica and their possible involvement in bioprotection. Plant Sci. 141:149-157 https://doi.org/10.1016/S0168-9452(98)00243-X
  25. Press, C. M., Wilson, M., Tuzun, S. and Kloepper, J. W. 1997. Salicylic acid produced by Serratia marcescens 90-166 is not the primary determinant of induced systemic resistance in cucumber or tobacco. Mol. Plant-Microbe Interact. 6:761-768
  26. Siegrist, J., Orober, M. and Buchenauer, H. 2000. $\beta$-Aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salicylic acid. Physiol. Mol. Plant Pathol. 56:95-106 https://doi.org/10.1006/pmpp.1999.0255
  27. Somssich, I. E. and Hahlbrock, K. 1998. Pathogen defense in plants-a paradigm of biological complexity. Trends in Plant Sci. 3:86-90 https://doi.org/10.1016/S1360-1385(98)01199-6
  28. Sticher, L., Mauch-Mani, B. and Metraux, J. P. 1997. Systemic acquired resistance. Annu. Rev. Phytopathol. 35:235-270 https://doi.org/10.1146/annurev.phyto.35.1.235
  29. Stromberg, A. and Brishammar, S. 1993. A histological evaluation of induced resistance to Phytophthora irfestans (Mont.) de Bary in potato leaves. J. Phytopathol. 137: 15-25 https://doi.org/10.1111/j.1439-0434.1993.tb01321.x
  30. Trotta, A., Varese, G. C., Gnavi, E., Fusconi, A., Sampo, S. and Berta, G. 1996. Interactions between the soil-borne root pathogen Phytophthora nicotianae vat parasitica and the arbuscular mycorrhizal fungus Glomus mosseae in tomato plnats. Plant Soil 185:199-209 https://doi.org/10.1007/BF02257525
  31. van Driesche, R. G. and Bellows, T. S. 1996. Biological control. New York, Chapman, Hall
  32. van Loon, L. C. 1997. Induced resistance in plants and the role of pathogenesis-related proteins. Eur. J. Plant Pathol. 103:753-765 https://doi.org/10.1023/A:1008638109140
  33. van Loon, L. C., Bakker, P. A. H. M. and Pieterse, C. M. J. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36:453-483 https://doi.org/10.1146/annurev.phyto.36.1.453
  34. van Wees, S. C. M., Pieterse, C. M. J., Trijssenaar, A., Van't Westende, Y. A. M., Hartog, F. and Van Loon, L. C. 1997. Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria. Mol. Plant-Microbe Interact. 6:716-724
  35. Vigo, C, Norman, J. R. and Hooker, J. E. 2000. Biocontrol of the pathogen Phytophthora parasitica by arbuscular mycorrhizal fungi is a consequence of effects on infection loci. Plant Pathol. 49:509-514 https://doi.org/10.1046/j.1365-3059.2000.00473.x
  36. Zimmerli, L., Jakab, G. Metraux, J. P. and Mauch-Mani, B. 2000. Potentiation of pathogen-specific defense mechanisms in Arabidopsis by $\beta$-aminobutyric acid. Proc. Natl. Acad. Sci. USA 97: 12920-12925

Cited by

  1. Defense Priming: An Adaptive Part of Induced Resistance vol.68, pp.1, 2017, https://doi.org/10.1146/annurev-arplant-042916-041132
  2. The arbuscular mycorrhizal symbiosis promotes the systemic induction of regulatory defence-related genes in rice leaves and confers resistance to pathogen infection vol.13, pp.6, 2012, https://doi.org/10.1111/j.1364-3703.2011.00773.x
  3. Comparative Ultrastructure of Cucumbers Pretreated with Plant Growth-promoting Rhizobacteria, DL-3-aminobutyric Acid or Amino Salicylic Acid after Inoculation with Colletotrichum orbiculare vol.155, pp.7-8, 2007, https://doi.org/10.1111/j.1439-0434.2007.01252.x
  4. The arbuscular mycorrhiza fungusRhizophagus irregularisMUCL 41833 decreases disease severity of Black Sigatoka on banana c.v. Grande naine, underin vitroculture conditions vol.70, pp.1, 2015, https://doi.org/10.1051/fruits/2014041
  5. Defence mechanisms associated with mycorrhiza-induced resistance in wheat against powdery mildew vol.44, pp.4, 2017, https://doi.org/10.1071/FP16206
  6. Ultrastructures ofColletotrichum orbicularein the Leaves of Cucumber Plants Expressing Induced Systemic Resistance Mediated byGlomus intraradicesBEG110 vol.36, pp.4, 2008, https://doi.org/10.4489/MYCO.2008.36.4.236
  7. Effects of β-aminobutyric acid on control of postharvest blue mould of apple fruit and its possible mechanisms of action vol.61, pp.2-3, 2011, https://doi.org/10.1016/j.postharvbio.2011.02.008
  8. Mycorrhiza-Induced Resistance and Priming of Plant Defenses vol.38, pp.6, 2012, https://doi.org/10.1007/s10886-012-0134-6
  9. Mycorrhiza induced resistance in potato plantlets challenged by Phytophthora infestans vol.76, pp.1, 2011, https://doi.org/10.1016/j.pmpp.2011.06.005
  10. β-Aminobutyric acid induces resistance of mango fruit to postharvest anthracnose caused by Colletotrichum gloeosporioides and enhances activity of fruit defense mechanisms vol.160, 2013, https://doi.org/10.1016/j.scienta.2013.05.023
  11. Phosphorus supply, arbuscular mycorrhizal fungal species, and plant genotype impact on the protective efficacy of mycorrhizal inoculation against wheat powdery mildew vol.26, pp.7, 2016, https://doi.org/10.1007/s00572-016-0698-z