Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect on Inhibition of Matrix Metalloproteinase-1 in Human Dermal Fibroblasts by Production of Exopolysaccharide from Mycelial Culture of Grifola frondosa

잎새버섯이 생산하는 세포외 다당체의 사람 섬유아세포에서 Matrix Metalloproteinase-1 발현저해 효과

  • Published : 2005.06.01
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Abstract

We investigated the effect on inhibition of matrix metalloproteinase (MMP) in human dermal fibroblast (HDF) by production of exopolysaccharide (GF-glucan) from mycelial culture of Grifola frondosa HB0071. The photoprotective potential of GF-glucan was tested in HDF exposed to ultraviolet-A (UVA) light. It was revealed that GF-glucan had an inhibitory effect on MMP-1 expression in UVA-irradiated HDF without any significant cytotoxicity. The treatment of UVA-irradiated HDF with GF-glucan resulted in a dose-dependent degrease in the expression level of MMP-1 protein and mRNA (by maximum $54.4\%$ at an $0.5\%$ GF-glucan). These results suggest that GF-glucan obtained from mycelial culture of G. frondosa HB0071 may contribute to inhibitory action in photoaging by reducing the MMP-1 related matrix degradation system.

잎새버섯(Grifola frondosa HB0071)이 생산하는 세포외 다당체인 GF-glucan이 사람섬유아세포에서 자외선(UVA)조사 시 증가되는 MMP-1 발현에 미치는 영향을 조사하였다. 자외선으로부터 조사된 섬유아세포에 GF-glucan을 처리한 농도에 따라 MMP-1 발현이 억제되었으며, RT-PCR를 이용해 세포내 MMP-1 mRNA 발현 또한 감소하는 것으로 나타났다 즉, 최대 GF-glucan $0.5\%$를 처리했을 때 $54.4\%$의 MMP-1 발현을 억제하였다. 결과적으로, 잎새버섯 HB0071로부터 생산된 GF-glucan은 피부노화와 관련된 extracellular matrix (ECM) 조직에 손상을 주는 MMP-1의 활성을 억제하여 자외선으로부터 손상된 피부의 광노화로부터 보호해주는 것으로 밝혀졌다.

Keywords

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