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Preparation of Accelerated Salt-fermented Anchovy Sauce Added with Shrimp Byproducts

새우가공부산물을 이용한 속성 멸치액젓의 제조

  • Kim, Hye-Suk (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Yang, Soo-Kyeong (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Chan-Ho (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Han, Byung-Wook (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Kang, Kyung-Tae (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Ji, Seung-Gil (Daesang Food Co) ;
  • Sye, Youn-Eon (The Great Desire of Anchovy Village, Tongyeong) ;
  • Heu, Min-Soo (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jin-Soo (Division of Marine Bioscience, and Institute of Marine Industry, Gyeongsang National University)
  • 김혜숙 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 양수경 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 박찬호 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 한병욱 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 강경태 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 지승길 (대상식품(주)) ;
  • 서윤언 (멸치마을) ;
  • 허민수 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 김진수 (경상대학교 해양생물이용학부/해양산업연구소)
  • Published : 2005.10.01

Abstract

The purpose of this study was to prepare accelerated salt-fermented anchovy sauce using a shrimp processing byproducts (head, shell and tail) as a fermenting accelerator, and to investigate its physicochemical and enzymatic properties. Four types of sauces were prepared with 0, 10, 20, and 30$\%$ (w/w) addition of shrimp byproduct and fermented at 24$\pm$2$^{\circ}C$ for 360 days. During fermentation, all four type sauces decreased moisture content (67.5$\%$68.0$\%$ to 64.0$\∼$64.8$\%$) and pH (5.52$\∼$7.10 to 5.03$\∼$6.58), but showed increase in their crude protein (7.0$\∼$8.2 to 10.8$\%$) and volatile basic nitrogen contents (40$\∼$75 to 180$\∼$200 mg/100 g of sauce). The ratio of amino nitrogen to total nitrogen contents of control (0$\%$) and sauce with 10$\%$ shrimp byproducts (10$\%$ sauce) were maximized at 270 days, whereas 20$ \% $ and 30$\%$ added sauces were at 180 days. Endoprotease and exoprotease activities of anchovy sauces added with 20$\%$ and 30$\%$ of shrimp byproducts tend to be higher than those of control (0$\%$) and 10$\%$ addition. Proteolytic activities of sauces at pH 9 were about 2 times higher than those at pH 6. Amidolytic activities for LeuPNA decreased remarkably during fermentation, and control (0$\%$) almost lost their activity at 180 days, while additional sauces were relatively stable. These suggest that alkaline pretense of anchovy and shrimp byproducts as a endoprotease mainly contributed to the fermentation of salt-fermented sauces. The protein molecular weight distribution of sauces indicated 2 groups of peaks (peak 1,>70,000 da and peak 2, 3,000$\∼$29,000 da). As the fermentation proceeded, peak 1 tended to decrease in all of sauces, but peak 2 increased rapidly from 30 to 270 days. Optimum fermentation periods of control and 10$\%$ sauces were 270 days and those of 20$\%$ and 30$\%$ sauce were 180 days. The results suggest that shrimp byproduct can be used as accelerator of salt-fermented sauce.

새우 가공부산물(두부, 갑각 및 꼬리)을 이용한 멸치 액젓의 발효기간 단축 및 기호성 개선에 의한 고품질의 속성 멸치 액젓을 제조할 목적으로 새우 가공부산물을 마쇄 하고, 이를 멸치 마쇄물에 0$ \%$, 10$\%$, 20$\%$ 및 30$\%$를 각각 첨가한 다음 숙성 중 이들의 이화학적 특성, 효소학적 특성 및 수율 등을 검토하였으며, 아울러 이를 토대로 최적 숙성기간 및 첨가량의 구명을 시도하였다 새우 가공부산물의 첨가량에 관계없이 숙성 중 수분함량 및 pH의 경우 감소하는 경향을 나타내었고, 조단백질 함량, 휘발성염기질소 함량, 갈변도 및 수율의 경우 증가하는 경향을 나타내었으며, 염도의 경우 거의 변화가 없었다. 아미노질소 및 아미 노질소/총질소는 숙성 중 새우 가공부산물 0$\%$ 및 10$\%$ 첨가 제품의 경우 270일째까지 급격히 증가하는 경향을 나타내었으나, 새우 가공부산물 20$\%$ 및 30$ \%$ 첨가 제품의 경우 180일째까지 급격히 증가한 후 거의 변화가 없었다. 액젓 중의 endoprotease와 exoprotease 활성 및 gel filtration의 결과, 숙성기간이 경과함에 따라 멸치액젓은 저분자화 되었으며, 이러한 경향은 새우 가공부산물의 첨가량이 증가할수록 현저하였다. 새우 가공부산물 첨가 멸치액젓의 최적 숙성기는 무첨가 및 10$\%$ 첨가 제품의 경우 270일, 20$\%$ 및 30$\%$첨가 제품의 경우 180일로 판단되어, 새우 가공부산물의 경우 멸치액젓의 제조시 발효촉진제로 사용 가능하리라 판단되었다.

Keywords

References

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