Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Hydrolysis of Ginseng Saponins and Quantifications of Saponins, Prosapogenins and Sapogenins in Crude Drug Extracts for Quality Contyol

  • Published : 2005.09.01
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Abstract

Ginseng saponins have been known as main active principles and are quantified as the index components of ginseng and its products for quality control. However ginseng saponins are easily hydrolyzed in acidic solutions of crude drug preparations. Due to the hydrolysis of saponins in acidic condition, it is generally difficult to determine ginseng saponins In crude drug preparations. Ginseng saponins, prosapogenins and sapogenins of crude drug extracts were quantified by HPLC. Ginseng saponins were quantified by HPLC on $Lichrosorb-NH_2$ column with acetonitrile/water/1-butanol(80:20:10, v/v). Ginseng $prosapogenin-Rg_2$ and $-Rg_2$ were extracted with ethyl acetate from $50\%$ acetic acid hydrolyzates of saponin fractions and quantified by HPLC on $Lichrosorb-NH_2$ column with acetonitrile/water(90:10, v/v). Ginseng sapogenins, panafadiol and panaxatriol, were extracted with diethyl ether from $7\%-sulfuric$ acid hydrolyzates of saponin fractions and quantified by HPLC on ${\mu}-Bondapak\;C_{18}$ column with acetonitrile/methano1/chloroform(83:10:7, v/v). These methods of analyses of sapogenins and prosapogenins were more useful for quality control than those of ginseng saponins in some of crude drug preparations.

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References

  1. Brekhman, I.I. and Dardymov, I.V. : New substances of plant origin which increase nonspecific resistance. Ann. Rev. Pharm. 9, 419-443 (1969) https://doi.org/10.1146/annurev.pa.09.040169.002223
  2. Shibata, S.: Some chemical studies on ginseng. Proceedings of international ginseng symposium. The central Research Institute, Office of Monopoly, Seoul, p.69-76 (1974)
  3. Takagi, K.: Pharmacological studies on ginseng. Proceedings of international ginseng symposium. The central Research Institute, Office of Monopoly, Seoul, p.119-127 (1974)
  4. Shibata, S., Isokawa, H., Sankawa, U., Shoji, J. and Takido, M. : Natural products for medicinal use, Nanzando Company, Limited, Tokyo, p.386-445 (1982)
  5. Kasai, R., Besso, H., Tanaka, O., Saruwatari, Y. and Fuwa, T. : Saponins of red ginseng. Chem. Pharm. Bull., 31, 2120-2125 (1983) https://doi.org/10.1248/cpb.31.2120
  6. Kitagawa, l. : Chemical studies on crude drug processing red ginseng and white ginseng. Proceedings of the 4th international ginseng symposium, Korea Ginseng and Tobacco Research Institute, Daejeon, Korea, p. 159- 168 (1984)
  7. Soldati, F. : HPLC seperation and quantitative determination of ginsenosides from Panax Ginseng, Panax Quinquefolium and from ginseng drug preparations. Proceedings of the 3rd international ginseng symposium, Korea Ginseng and Tobacco Research Institute, Seoul, Korea, p.59-69 (1980)
  8. Choi, K.J., Ko, S.R., Kim, S.C. and Park, J.D. : Saponin and ginsenoside content in korean red ginseng products. Korean J. Ginseng Sci. 13, 178-182 (1989)
  9. Kaku, T. and Kawashima, Y : Isolation and characterization of ginsenoside-$Rg_2$, 20R-prosapogenin, 20S-prosapogenin and $\{Delta}^{20}$-prosapogenin. Arzneim. -Forsh./Drug Res., 30,936-943 (1980)
  10. Choi, K.J., Ko, S.R., Kim, S.C. and Kim, M.W. : Idetification and determination of ginseng saponins, prosapogenins and sapogenins from crude drug preparations for quality control. Proceedings of the 6th international ginseng symposium, Korea Ginseng and Tobacco Research Institute, Seoul, Korea, p.206-214 (1993)
  11. Choi, K.J., Ko, S.R., Jeon, B.S. and Sung, H.S. : Identification of ginseng sapogenin and quantitative determination of ginsenoside-$Rb_1$. from crude drug preparation containing Bupleuri Radix. Kor. J.Pharmacogn. 20, 175-179.(1989)
  12. Choi, K.J., Ko, S.R., Kim, N.M. and Sung, H.S. : Identification of ginseng saponin and quantitative determination of ginsenoside-$Rb_1$, from crude drug preparation drink. Korean J. Ginseng Sci. 14, 112-116 (1990)
  13. Kwak, Y.S., Choi, K.J. and Kim, N.M. : Effect of ozone treatment on the changes of fatty acids, organic acids, and sensory properties in ginseng powder. J. Fd. Hyg. Safety 11, 51-55 (1996)