Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Electrical Recognition of Label-Free Oligonucleotides upon Streptavidin-Modified Electrode Surfaces

  • Park, Jong-Wan (The Institute of Scientific and Industrial Research, Osaka University) ;
  • Jung, Ho-Sub (The Institute of Scientific and Industrial Research, Osaka University) ;
  • Lee, Hea-Yeon (The Institute of Scientific and Industrial Research, Osaka University) ;
  • Kawai, Tomoji (The Institute of Scientific and Industrial Research, Osaka University)
  • Published : 2005.12.31
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Abstract

For the purpose of developing a direct label-free electrochemical detection system, we have systematically investigated the electrochemical signatures of each step in the preparation procedure, from a bare gold electrode to the hybridization of label-free complementary DNA, for the streptavidin-modified electrode. For the purpose of this investigation, we obtained the following pertinent data; cyclic voltammogram measurements, electrochemical impedance spectra and square wave voltammogram measurements, in $Fe(CN)_6^{3-}/Fe(CN)_6^{4-}$ solution (which was utilized as the electron transfer redox mediator). The oligonucleotide molecules on the streptavidin-modified electrodes exhibited intrinsic redox activity in the ferrocyanide-mediated electrochemical measurements. Furthermore, the investigation of electrochemical electron transfer, according to the sequence of oligonucleotide molecules, was also undertaken. This work demonstrates that direct label-free oligonucleotide electrical recognition, based on biofunctional streptavidin-modified gold electrodes, could lead to the development of a new biosensor protocol for the expansion of rapid, cost-effective detection systems.

Keywords

References

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