Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-tumor Activity of Fermented Liquid Opuntia humifusa in Cervical Cancer Cells and Its Chemical Composition

손바닥 선인장(Opuntia humifusa) 발효액의 화학적 성분과 자궁경부암 세포주에 대한 항암작용

  • Choi, Hwa-Jung (Dept. of Interdisciplinary Program in Agricultural Biotechnology, Seoul National University) ;
  • Park, Seung-Chun (College of Vererinary Medicine, Kyungpook National University) ;
  • Hong, Tae-Hee (Dept. of Food Nutrition & Daejeon Health Sciences College)
  • 최화정 (서울대학교 협동과정 농업생물공학) ;
  • 박승춘 (경북대학교 수의과대학 약리학실험실) ;
  • 홍태희 (대전보건대학 식품영양과)
  • Published : 2005.12.01
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Abstract

The purpose of this study is to investigate anti-tumor activities, general composition, elemental composition and mineral contents of fermented liquid stem, root and fruit of Opuntia humifusa. In the general composition, the energy, crude protein, crude lipid and crude carbohydrate contents of fermented liquid stem were 86.21 Kcal, 0.92$\%$, 0.12$\%$, and 20.34$\%$, respectively. Fermented liquid fruit showed 65.32 Kcal, 1.04$\%$, 0.08$\%$, and 15.15$\%$. In mineral analysis, fermented liquid stem and fruit showed 1,800 and 388 mg of calcium per 100g. The ferrous concentrations of fermented liquid stem and fruit were 21 and 10 mg per 100 g, respectively. Methanol, ethanol and water extracts of nonfermented liquid stem and fruit did not inhibit the proliferation in human cervical cancer cells (Caski, SiHa and HaCaT), but the fermented liquid fruit showed the inhibition of Proliferation with dose-response manner in Caski and SiHa cells, but not HaCaT. Therefore, it suggests that fermented cactus may be used as one of potential adjuvant for the treatment of cervical carcinomas.

손바닥선인장(Opuntia humifusa)의 줄기, 뿌리 그리고 잎 발효액의 화학성분 비교 및 자궁경부암 세포주에 대한 항암 활성을 조사하였다. 줄기 발효액의 열량, 조단백, 조지방 그리고 조탄수화물의 성분비율은 86.21 kcal, $0.92{\%}$, $0.12{\%}$ 그리고 $20.34{\%}$로 분석되었다. 열매에서는 65.32 kcal, $0.08{\%}$, $1.04{\%}$ 그리고 $15.15{\%}$로 각각 구성되었다. 무기물 분석에서 줄기와 열매의 발효액에 칼슘(calcium)과 철(ferrous)의 농도는 100g 당 1,800 mg과 388 mg 그리고 21 mg과 10 mg으로 분석 되었다. 발효 전에 물, 메탄올 그리고 에탄올 추출물에 대하여 항암활성을 측정한 결과 자궁경부암 세포주인 Caski, SiHa 그리고 HaCaT에서 어떠한 항암활성도 보여주지 않았다. 그러나 손바닥선인장(Opuntia humifusa)의 열매를 효모균으로 발효한 발효액에서 HaCaT를 제외한 CaSki와 SiHa에 대해서는 항암활성을 보여주었다.

Keywords

References

  1. Miquel J, Quintanilha AT, Weber H. 1989. Handbook of free radicals and antioxidants in biomedicine. CRC Press, London. Vol I, p 223-244
  2. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extract. Korean J Food Sci Technol 27: 978-984
  3. Yoon JY, Song MR, Lee SR. 1990. Comparison of antithiamine acttvitres of wild vegetables. Korean J Food Sci Technol 20: 808-811
  4. Lee YK, Lee HS. 1990. Effects of onion arid ginger on the liquid peroxidation and fatty acid composition of mackerel during frozen storage. J Korean Soc Food Nutr 19: 321-329
  5. Park PS, Lee BR, Lee MY. 1994. Effect of onion juice on ethanol induced hepatic lipid peroxidation in rats. J Korean Soc Food Nutr 23: 750-756
  6. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27: 80-85
  7. Choi U, Shin DH, Chang YS, Shin JL. 1992. Screening of natural antioxidant from plants and their anti oxidative effect. Korean J Food Sci Technol 24: 142-148
  8. Jamal NB, Ibrahim AW. 1994. Citric acid and antimicrobial affect microbiological stability and quality of tomato juice. J Food Sci 59: 130-134 https://doi.org/10.1111/j.1365-2621.1994.tb06916.x
  9. 이시진. 1994. 본초강목. 의성당, 서울
  10. Shin TK, Lee SJ, Kim SJ. 1998. Effects of Opuntia ficusindicae xtract on the activation of immune cells with special reference to autoimmune disease models. Korean J Vet Pathol 2: 31-35
  11. Lee HJ. 1997. A study on antiulcer effects of Opuntia dillenii Haw. on stomach ulcer induced by water-immersion stress in rats. MS Thesis. Seoul National University
  12. Florian CS, Reinhold C. 2005. Catus stems (Opuntia spp.): Areview on their chemistry, technology, and uses. Mol Nutr Food Res 49: 175-194 https://doi.org/10.1002/mnfr.200400071
  13. Paik S. 1998. Effects of Opuntia ficus-indica furit on the passive avoidance and anti-oxidation. in the senescence-acceerated mouse. MS thesis. Cheju National University
  14. Goldstein G, Nobel PS. 1994. Water relations and low-temperature acclimation for Cactus species varying in freezing tolerance. Plant Physiol 104: 675-681 https://doi.org/10.1104/pp.104.2.675
  15. AOAC. 1980. Official Method of Analysis. 15th ed. Association of official analytical chemists, Washington DC
  16. Lee YJ, 2004. A study on mineral and alginic acid contents by different parts of sea mustards (Undaria pinnatifida). Korean J Food Cul 19: 691-700
  17. Skehan P, Storeng S, Studiero D, Monke A, McKahon J, Vistica D, Warren J, Bodesh H, Kenny S, Boyd MR. 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 82: 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  18. Sohn KS, Lee JH, Ha YS. 2002. Clarification of mixed fruit and vegetable juices. Food Eng Prog 6: 241-247
  19. Jeong YJ, Lee GD, Lee MH, Uea MJ, Lee GH, Choi SY. 1999. Monitorin on pectinase treatment conditions for clarification of persimmon vinegar. J Korean Soc Food Sci Nutr 28: 810-815
  20. Ishill S, Yokotsuka T. 1972. Clarification of fruit juice by pectin trans-eliminase. J Agric Food Chem 20: 787-791 https://doi.org/10.1021/jf60182a017
  21. Nelson PE, Tressler DK. 1980. Fruit and vegetable juice processing technology. AVI, Westport, CT
  22. Chun YK, Choi HS, Cha BS, Oh HI, Kim WJ, 1997. Effect of enzymatic hydrolysis on the physicochemical properties of persimmon juice. Korean J Food Sci Technol 29: 198-203
  23. Ko EJ, Choi YH. 1999. Clarification of grape juice by ultra-filtration and membrane fouling characteristics. Food Eng Prog 3: 57-63
  24. Kang HA, Chang KS, Min YK, Choi YH. 1998. Value addition of jujube wine using microfiltration and ultrafiltration. Korean J Food Sci Technol 30: 1146-1151
  25. Lee EY, Woo GJ, 1998. Optimization of separation process of bioflavonoids and dietary fiber from tangrine peels using hollow fiber medpsjwlmbrane. Korean J Food Sci Technol 30: 151-160

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