Genetic Divergence and Relationship Among Four Abalone Species by Isozyme and AFLP analyses

Isozyme 및 AFLP분석에 의한 전복류 4종간의 유전적 차이 및 유연관계

  • Park Choul-ji (Shellfish Genetic and Breeding Research Center, NFRDI) ;
  • Kijima Akihiro (Education and Research Center of Marine Bio-Resources, Tohoku University)
  • Published : 2005.11.01

Abstract

Isozyme and AFLP analyses were examined to estimate the utilities of them as a genetic marker. The utilities were evaluated by genetic divergence and relationships among the four distinct abalone species; Haliotis discus hannai collected from northeast coast of Japan and Yellow-Sea coast of China, H. rufescens collected from west coast of USA, H rubra collected from southeast coast of Australia and H midae collected from Cape Town of South Africa. Isozyme and AFLP analyses showed a clear genetic divergence between every pair of species. Genetic relationships among the low species estimated by isozyme and AFLP analyses reflected to geographical distribution and morphological characteristics. In conclusion, Isozyme and AFLP analyses are suitable genetic markers far estimates of genetic divergence and relationship among abalone species.

Isozyme과 AFLP분석에 있어 종간의 유전적 차이 및 유연관계를 분석에 따른 각 유전표식의 특징 및 유용성을 파악하기 위하여 종으로 명확히 구분되는 북반구 2종(H. discus hannai와 또 rufescens)과 남반구 2종(H rubra와 H midae)을 대상으로 분석한 결과 Isozyme 및 AFLP분석은 전복류의 종간분석에 있어 유전적 차이 및 유연관계 분석에 유용한 유전표식임을 시사하였다.

Keywords

References

  1. Ayala, F. J., 1982. Population and Evolutionary Genetics: A Primer, pp. 190-197
  2. Barrett, B. A. and K. K. Kidwell, 1998. AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest. Crop Sci., 38, 1261-1271 https://doi.org/10.2135/cropsci1998.0011183X003800050025x
  3. Brown, L. D., 1993. Biochemical Genetics and Species Relationships within the Genus Haliotis (Gastropoda: Haliotidae). J. Moll. Stud., 59, 429-443 https://doi.org/10.1093/mollus/59.4.429
  4. Fujio, Y., 1984. Study on genetic characteristics of fish and shellfishes in isozyme analysis. Nosuisho Tokubetsu Shiken, pp. 15-25
  5. Garcia-Mas, J., M. Oliver, H. Gomez-Paniagua and M. C. de Vicente, 2000. Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor. Appl. Genet., 101, 860-864 https://doi.org/10.1007/s001220051553
  6. Gilbert, D. A., N. Lehman, S. J. O'Brien and R. K. Wayne, 1990. Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature, 344, 764-767 https://doi.org/10.1038/344764a0
  7. Lin, J. J. and J. Kuo, 1995. $AFLP^{TM}$: A novel PCR-based assey for plant and bacterial DNA fingerprinting. Focus, 17, 66-70
  8. Lindberg, D. R, 1992. Evolution distribution and systematics of Haliotidae. (in) Abalon of the World: Biology, Fisheries and Culture (ed.), S. A. Shepherd, M. Tenger & S. Guzman, Black-wells, London, pp. 3-18
  9. Mickeet, K., C. Morton, J. Feng, P. Li, M. Simmons, D. Cao, R. A. Dunham and Z. Liu, 2003. Assessing genetic diversity of domestic population of channel catfish (Ictalurus punctatus) in Alabama using AFLP markers. Aquaculture, 228, 91-105 https://doi.org/10.1016/S0044-8486(03)00311-9
  10. Nei, M., 1972. Genetic distance between population. Amer. Natur., 106, 283-292 https://doi.org/10.1086/282771
  11. Nei, M., 1975. Molecular population genetic and evolution North-Holland, Amsterdam and New York, 288 pp
  12. Oniwa, K. and A. Kijima, 1989. Genetic distance between species in marine bivalves. Fish Genet. Breed. Sci., 14, 27-32
  13. Park, J. Y. and A. Kijima, 1991. Estimation of Interspecific Gene Differences among Pleuronectid species with a Minimum Number of Isozyme Loci. Tohoku J. Agr. Res., 42, 34-53
  14. Sneath, P. H. A. and R. R. Sokal, 1973. The principles and practice of numerical classification. Numerical taxonomy, pp. 1-15
  15. Sokal, R. R. and P. H. A. Sneath, 1963. Principles of numerical taxonomy. 1st ed. W. H. Freeman and Co., San Francisco, CA, 312 pp
  16. Takagi, M., G. Sogabe and N. Taniguchi, 1998 Genetic Variability and Divergence of Ayu Plecoglossus altivelis Using AFLP Fingerprinting. Fish Genet. Breed. Sci., 26, 55-61
  17. Taniguchi, N. and K. Takagi, 1997. DNA Polymorphisms and Diversity analysis of Fish population. (in) DNA of Fish, pp. 117-137
  18. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van der Lee, M. Homes, A. Frijters, J. Pot, J. Peleman, M. Kupier and M. Zabeau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res., 23, 4407-4414 https://doi.org/10.1093/nar/23.21.4407
  19. Yue, G H., Y. Li, L. C. Lim and L. Orban, 2004. Monitoring the genetic diversity of three Asian arowana (Scleropages formosus) captive stocks using AFLP and microsatellites. Aquaculture, 237, 89-102 https://doi.org/10.1016/j.aquaculture.2004.04.003