Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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Zinc Deficiency Decreased Alkaline Phosphatase Expression and Bone Matrix Ca Deposits in Osteoblast-like MC3T3-E1 Cells

  • Cho Young-Eon (Department of Food Science and Nutrition, Andong National University) ;
  • Lomeda Ria-Ann R. (Department of Food Science and Nutrition, Andong National University) ;
  • Kim Yang-Ha (Department of Food Science and Nutrition, Ewha Womens University) ;
  • Ryu Sang-Hoon (Department of Central laboratory for Instrumental Analysis, Andong National University) ;
  • Choi Je-Yong (Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Kim Hyo-Jin (Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Beattie John H. (Cellular Integrity Division, Rowett Research Institute) ;
  • Kwun In-Sook (Department of Food Science and Nutrition, Andong National University)
  • Published : 2005.11.01
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Abstract

It is well established that zinc plays an important role in bone metabolism and mineralization. The role of zinc in bone formation is well documented in animal models, but not much reported in cell models. In the present study, we evaluated zinc deficiency effects on osteoblastic cell proliferation, alkaline phosphatase activity and expression, and extracellular matrix bone nodule formation and bone-related gene expression in osteoblastic MC3T3-E1 cells. To deplete cellular zinc, chelexed-FBS and interpermeable zinc chelator TPEN were used. MC3T3-E1 cells were cultured in zinc concentration-dependent (0-15 ${\mu}M\;ZnCl_2$) and time-dependent (0-20 days) manners. MC3T3-E1 cell proliferation by MTT assay was increased as medium zinc level increased (p<0.05). Cellular Ca level and alkaline phosphatase activity were increased as medium zinc level increased (p<0.05). Alkaline phosphatase expression, a marker of commitment to the osteoblast lineage, measured by alkaline phosphatase staining was increased as medium zinc level increased. Extracellular calcium deposits measured by von Kossa staining for nodule formation also appeared higher in Zn+(15 ${\mu}M\;ZnCl_2$) than in Zn-(0 ${\mu}M\;ZnCl_2$). Bone formation marker genes, alkaline phosphatase and osteocalcin, were also expressed higher in Zn+ than in Zn-. The current work supports the beneficial effect of zinc on bone mineralization and bone-related gene expression. The results also promote further study as to the molecular mechanism of zinc deficiency for bone formation and thus facilitate to design preventive strategies for zinc-deficient bone diseases.

Keywords

References

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