The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of Potato virus Y Isolated from Paprika in Korea

  • Choi, Hong-Soo (Department of Plant Pathology, National Institute of Agricultural Science and Technology) ;
  • Ko, Sug-Ju (Jeonnam Agricutural Research & Extension Services) ;
  • Kim, Mi-Kyeong ;
  • Park, Jin-Woo (Department of Plant Pathology, National Institute of Agricultural Science and Technology) ;
  • Lee, Su-Heon (Department of Plant Pathology, National Institute of Agricultural Science and Technology) ;
  • Kim, Kook-Hyung (School of Agricultural Biotechnology, Seoul National University) ;
  • Were, Hassan Karakacha (Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University) ;
  • Chois, Jang-Kyung (Department of Agricultural Biology, Kangwon National University) ;
  • Takanami, Yoichi (Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University)
  • Published : 2005.12.01
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Abstract

A virus isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Potato virus Y (PVY) based on biological, serological, cytopathological, and molecular properties. In host range studies, the paprika isolate produced the mosaic symptom on some tobacco, tomato and pepper (Capsicum annuum). A new paprika isolate also infected potato cultivars which is different biological characteristic compared to the other popular potyvirus infecting paprika, Pepper mottle virus (PepMoV). Previously reported PVY strains, $PVY^o$ and $PVY^N$ did not infect pepper and typical PepMoV isolates did not infect potato. Distinctive inclusion patterns of the scroll, pinwheel, long laminated inclusions, and helper components in the cytoplasm of infected cells were also different to those observed by the typical PVY isolate infections. However, the paprika isolate reacted to the monoclonal antibody of $PVY^N$ strain with high absorbance readings. RT-PCR amplification, cloning, and sequencing of the 3' untranslated region and a part of coat protein gene also added additional evidence of the paprika isolate as the $PVY^N$-related isolate. Multiple alignments as well as cluster dendrograms of PVY-paprika isolate revealed close phylogenetic relationship to the $PVY^N$ subgroup. Altogether, these results suggest that a new PVY isolate infecting paprika contained distinct characteristics compared to the other previously described PVY strains with closer relationship to the $PVY^N$ strain.

Keywords

References

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