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Induction of Quinone Reductase, an Anticarcinogenic Marker Enzyme, by Extract from Chrysanthemum zawadskii var. latilobum K.

  • Kim, Ju-Ryoung (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jung-Hyun (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lim, Hyun-Ae (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jang, Chan-Ho (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jang-Hoon (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kwon, Chong-Suk (Department of Food Science and Nutrition, Andong National University) ;
  • Kim, Young-kyun (Department of Forest Resources, Kookmin University) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Published : 2005.12.01

Abstract

Induction of NAD(P)H:(quinone-acceptor) oxidoreductase (QR) which promotes obligatory two electron reduction of quinones and prevents their participation in oxidative cycling and thereby the depletion of intracellular glutathione, has been used as a marker for chemopreventive agents. Induction of phase II enzyme is considered to be an important mechanism of cancer prevention. In our previous study, we assessed the quinone reductase QR-inducing activities of 216 kinds of medicinal herb extracts in cultured murine hepatoma cells, BPRc1 and hepalc1c7 cells. Among the 216 herbal extracts tested in that study, extracts from Chrysanthemum zawadskii showed significant induction of QR. In this study, we examined QR-inducing activity of solvent fractions of the herbal extract. The dichloromethane fraction of the herb showed the highest QR induction among the samples fractionated with four kinds of solvents with different polarity. The fraction also significantly induced the activity of glutathione S-transferase (GST), one of the major detoxifying enzymes, at $4{\mu}g/mL\;and\;2{\mu}g/mL$ in hepalc1c7 and BPRc1 cells, respectively. In conclusion, dichloromethane-soluble fraction of Chrysanthemum zawadskii which showed relatively strong induction of detoxifying enzymes merits further study to identify active components and evaluate their potential as cancer preventive agents.

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Cited by

  1. Protection by Chrysanthemum zawadskii extract from liver damage of mice caused by carbon tetrachloride is maybe mediated by modulation of QR activity vol.4, pp.2, 2010, https://doi.org/10.4162/nrp.2010.4.2.93
  2. Volatiles of Chrysanthemum zawadskii var. latilobum K. vol.17, pp.3, 2012, https://doi.org/10.3746/pnf.2012.17.3.234