Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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A Real Time PCR Assay for Detection and Quantitation of Canine Parvovirus Type 2 in the Feces of Dogs with Parvovirus Infection

실시간 중합효소연쇄반응법을 이용한 개 파보바이러스 감염증의 분변에서 바이러스 정량 분석

  • koh Min-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Sin So-Yeon (College of Veterinary Medicine, Chonnam National University) ;
  • Kim Yong-Hwan (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Koh Ba-Ra-Da (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Lee Bong-Joo (College of Veterinary Medicine, Chonnam National University)
  • 고민수 (전남대학교 수의과대학 수의학과) ;
  • 신소연 (전남대학교 수의과대학 수의학과) ;
  • 김용환 (광주광역시 보건환경연구원) ;
  • 고바라다 (광주광역시 보건환경연구원) ;
  • 이봉주 (전남대학교 수의과대학 수의학과)
  • Published : 2005.12.01
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Abstract

We described a rapid, sensitive and reproducible real-time PCR assay for detection and quantitation of canine parvovirus type 2 in the feces of dogs with parvovirus infection. The method was demonstrated to be highly specific and sensitive, allowing a precise canine parvovirus type-2 quantitation over range of eight orders of magnitude from $10^2\;to\;10^9$ copies of standard DNA. Then, fecal samples from parvovirus infected dogs were analyzed by conventional PCR and real-time PCR. Real-time PCR is more sensitive than conventional PCR, allowing to detect low viral titers of CPV-2 in infected dogs. By real-time PCR, a wide range of parvovirus particles was found in the samples from $1.45\times10^6\;to\;9.45\times10^8$ copies/0.01g of feces. However, when dogs are in infection of parvovirus, it is difficult to prove that the numbers of peripheral blood leukocytes are correlated with those of fecal shedding virus.

Keywords

References

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