Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지

Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

  • Choi Ki Young (Northern Kyungbuk Resion Bio Industry Innovation Center, Dong Yang University) ;
  • Son Sung Ho (Vitrosys, Co. Ltd) ;
  • Lee Joo Hyun (Department of Environmental Horticulture, The University of Seoul) ;
  • Lee Yong-Beom (Department of Environmental Horticulture, The University of Seoul) ;
  • Bae Jong Hyang (Division of Plant and Resource Science, Wonkwang university)
  • Published : 2005.12.01
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Abstract

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

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References

  1. Aitken-Christie, J. 1991. Automation In: Debergh PC & Zimmerman R.J. (eds) Micropropagation: Technol­ogy and Application. p. 363-388. Kluwer Academic Publishers, Dordrecht, The Netherlands
  2. Akita, M. and S. Takayama. 1988. Mass propagation of potato tubers using jar fermentor. Acta Hortic., 230:53-61
  3. Akita, M. and S. Takayama. 1993. Effects of medium surface level control on the mass production of potato tubers using a jar fermentor culture technique. Plant Tissue Culture Letters 10:242-248 https://doi.org/10.5511/plantbiotechnology1984.10.242
  4. Escalona, M., J.C. Lorenzo, B. Gonzalez, M. Daquinta, J. Gonzalez, Y. Desjardins, and C.G. Borroto. 1999. Pineapple (Ananas comosus L. Merr) micropropaga­tion in temporary immersion systems. Plant Cell Rep. 18:743-748 https://doi.org/10.1007/s002990050653
  5. Etienne, H., M. Lartaud, N. Michaux-Ferriero, M.P. Carron, M. Berthouly and C. Teisson. 1997. Improve­ment of somatic embryogenesis in Hevea brasiliensis (Mull, Arg.) using the temporary immersion tech­nique. In Vitro Cell. Dev. BioI. Plant. 33:81-87 https://doi.org/10.1007/s11627-997-0001-2
  6. Etienne, H. and M. Berthouly, 2002. Temporary immersion in plant micropropagation. Plant Cell Tiss. Org. Cult. 69:215-231 https://doi.org/10.1023/A:1015668610465
  7. Jo, M.H., I.K. Ham, M.A. Lee, N.H. Song, I.S. Woo and T.H. Rho. 2001. Effect of the culture vessel ecosystem on the growth and development of potato plantlets by bioreactors. J. Kor. Soc. Hort. Sci. 42(2): 128-130
  8. Jones, E.D. 1991. Progress in seed production tech­nique. Amer. Potato J. 68-247-248
  9. Joung, H. 1989. Mass production of potato microtuber by tissue culture technique and application. Agricul­tural Biotechnology Symposium. p. 100-124
  10. Forento, W.C. 1996. Growing media. : Types and physical/chemical properties, p. 93-122. In D.W. Reed (ed.) Water, media and nutrition for greenhouse crops. Ball Publishing, Batavia. Illinois, USA
  11. Hahn, E.J. and K.Y. Peak. 2005. Multiplication of chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. Plant Cell. Tiss. Org. Cult. 81:301-306 https://doi.org/10.1007/s11240-004-6655-0
  12. Kozai, T., K. Fujiwara, and Y. Kitaya. 1995. Model­ing, neasurement and control in plant tissue culture. Acta Hort. 393:63-73
  13. Schwarz, M. 1995. Nutrients. p. 7-32. In B.L. McNeal, F. Tardieu, H. Van Keulen, D. van Velck(eds). Soilless culture management. Springer-Verlag. Berlin, Heidel­berg, New York
  14. Son, S.H., S.M. Choi, S.R.Yun, O.W. Kwon, Y.H. Lee, and K.Y. Peak. 1999. Large-scale culture of plant cell and tissue by bioreactor system. J.Plant Biotechnol­ogy. 1:1-7
  15. Ziv. M., G. Meir, and A.H. Halevy. 1983. Factors influencing the production of hardened glaucous car­nation plantlets in vitro. Plant Cell Tiss. Org. Cult. 2:55-65 https://doi.org/10.1007/BF00033553