Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Gene cloning, tissue distribution, and its characterization of Ca2+-activated Cl- channel activated by ginsenosides in Xenopus laevis oocytes

Xenopus laevis oocytes에서 진세노사이드에 의하여 활성화되는 Ca2+-activated Cl- 이온 통로의 유전자 클로닝, 조직 분포 및 채널 특성

  • Jeong, Sang-Min (Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University) ;
  • Lee, Jun-Ho (Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University) ;
  • Yoon, In-Soo (Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University) ;
  • Nah, Seung-Yeol (Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University)
  • 정상민 (건국대학교 수의과대학 인삼학 및 생리학교실) ;
  • 이준호 (건국대학교 수의과대학 인삼학 및 생리학교실) ;
  • 윤인수 (건국대학교 수의과대학 인삼학 및 생리학교실) ;
  • 나승열 (건국대학교 수의과대학 인삼학 및 생리학교실)
  • Published : 2005.12.01
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Abstract

The $Ca^{2+}-activated$ chloride channel (CLCA) was activated by ginseng total saponin (GTS) in Xenopus oocytes. The reverse transcription PCR (RT-PCR) method was performed with gene specific primers on oocytes. The gene specific primers were deduced from spleen cDNA in expressed sequence tags (EST) database showing high homology to the mouse CLCA. Full length of cDNA sequence was completed by linkage of several 5' and 3'-half cDNA fragments have been sequenced. We named the full cDNA to oCLCA transiently. The oCLCA gene encodes a protein of 911 amino acids with $48.9\%$ identity overall to that of mouse CLCA (mCLCA4). A predicted oCLCA amino acids sequence shows the molecular weight of 108 kDa and has four or more transmembrane domains, and also the one hydrophobic C­terminal domain. oCLCA gene was expressed ubiquitously in various tissues included oocytes, also interfered in oocytes by siRNA for oCLCA. Here, we suggest that oCLCA is a endogenous chloride channel gene in oocytes. We are studying for the identification of oCLCA gene and further physiological research.

Keywords

References

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