Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effect of Caffeine on 3T3-L1 Adipocyte Differentiation : A Nutrigenomical Approach

Caffeine이 지방세포주 3T3-L1 분화에 미치는 영향: 영양유전체학적 접근

  • Kim Mi-Ja (Department of Obesity Management, Dongduk Women's University, Imagine Obesity Research Institute) ;
  • Kim Youngok (Department of Food and Nutrition, Dongduk Women's University) ;
  • Chung Joo-HO (Department of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Kim Jong-Woo (Imagine Obesity Research Institute, Department of Neuropsychiatry, College of Medicine, Kyung Hee University) ;
  • Kim Hye-Kyung (Department of Food and Biotechnology, Hanseo University)
  • 김미자 (동덕여자대학교 비만과학대학원 비만관리학과, 이매진 비만 의학연구소) ;
  • 김영옥 (동덕여자대학교 식품영양학과) ;
  • 정주호 (경희대학교 의과대학 약리학교실) ;
  • 김종우 (이매진 비만 의학연구소, 경희대학교 의과대학교 신경정신과) ;
  • 김혜경 (한서대학교 식품생물공학과)
  • Published : 2005.10.01
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Abstract

Nutrigenomics refers to research that investigates the interaction between nutrition and the human genome. Caffeine in tea and coffee is widely and routinely consumed by people. This study was performed to confirm the effect of caffeine treatment on the gene expression and cytokine profiling in 3T3-L1 adipocyte cells using microarray and protein array methodology. Treatment of caffeine in 3T3-L1 adipocyte cells increased expression of several genes related with obesity including adipocyte C1Q and collagen domain containing (ACDC), Adipsin (ADN), uncoupling protein 3(UCP3), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is known as lipid storage enzyme, was decreased by caffeine treatment. Furthermore, cytokines, such as interleukin-3 (IL-3), interleukin-12(IL-12), interleukin-13 (IL-13), granulocyte colony stimulating factor (GCSF), granulocyte macrophage colony stimulating factor (GM-CSF) and vascular endothelial growth factor (VEGF), were decreased in caffeine treated 3T3-L1 adipocyte cells. These results provided interesting information about the genes related with caffeine and cytokine expression profiling in obesity.

Keywords

References

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