Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison or Antioxidative Activities or Crotaiarta sessiflora L. Extracts from Leaves, Seed, Stem and Root

활나물 부위별 추출물의 항산화 활성 비교

  • Woo, NaRiYah (Dept. of Food Science and Nutrition, Hoseo University) ;
  • Kim, Tae-Su (Dept. of Food Science and Nutrition, Hoseo University) ;
  • Park, Hee-Woon (Rural Development Agricultural, National Institute of Crop Science) ;
  • Park, Chun-Geon (Rural Development Agricultural, National Institute of Crop Science) ;
  • Seong, Ha-Jeong (Safety Assesment Center, Korea Testing and Research institute for Chemical industry) ;
  • Ko, Sang-Heom (Safety Assesment Center, Korea Testing and Research institute for Chemical industry) ;
  • Jung, Jin-Woo (School of Culinary Art & Food Management, Yangsan University) ;
  • Kang, Myung-Hwa (Dept. of Food Science and Nutrition, Hoseo University)
  • 우나리야 (호서대학교 식품영양학과) ;
  • 김태수 (호서대학교 식품영양학과) ;
  • 박희운 (농촌진흥청 작물과학원) ;
  • 박춘근 (농촌진흥청 작물과학원) ;
  • 성하정 (한국화학시험연구원 안전성 평가본부) ;
  • 고상범 (한국화학시험연구원 안전성 평가본부) ;
  • 정진우 (영산대학교 식품영양학과) ;
  • 강명화 (호서대학교 식품영양학과)
  • Published : 2005.11.01
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Abstract

This study was carried to develop the new functional food material by exploring natural antioxidant substances of Crotalaria sessiflora L. We compared antioxidative activity of potential antioxidant substances extracted from Crotalaria sessiflora L. The order of extract yield of Crotalaria sessiflora L. were stem > loaves > seed > root. Antioxidative activities of Crotalaria sessiflora L. were measured by total polyphenol contents EDA (electron donating activity), SOD (superoxide dismutase) -like activity, hydroxyl radical scavenging ability and hydrogen peroxide radical scavenging ability. Total polyphenol acid content was much higher in leaves Ex than other extracts. And leaves Ex showed the most excellent antioxidative activity ($86.27\%$) in terms of SOD-like activity. The EDA was ordered loaves Ex > stem Ex > seed Ex > root Ex. Hydroxy radical scavenging ability was the most effective in loaves Ex, and hydorogen Peroxide radical scavenging ability was the highest in seed Ex. Therefore we could be certain that leaves Ex was the most effective in antioxidative activity from Crotalaria sessiflora L.

활나물의 항산화 효과를 측정하기 위하여 잎, 줄기, 뿌리 및 종자의 부위 별로 분리하여 methylen chloride와 ethanol 혼합용매에 의해 추출하였다. 활나물의 부위로 추출수율은 줄기 $4.47\%$, 잎 $3.95\%$, 종자 $2.90\%$, 뿌리 $2.67\%$의 순으로 줄기 부분의 추출수율이 가장 높았다. 총 페놀성 화합물 함량은 잎 2.98, 뿌리 2.34, 종자 2.26, 줄기 2.11 mg/mL로 잎추출물에서 가장 높았다. SOD 유사활성은 잎 추출물의 활성이 $86.27\%$로 가장 높았으며, 줄기와 뿌리 추출물은 각각 56.32, $50.29\%$이었으며, 종자 추출물은 경우 $16.82\%$로 가장 낮았다. 잎, 줄기, 뿌리 추출물의 SOD 유사활성은 천연항산화제 인 tocopherol보다 매우 높았다. 활나물 부위별 추출물의 전자공여능은 잎과 줄기 추출물에서 각각 16.87, $16.06\%$로 매우 높았으며, 뿌리 추출물과 종자 추출물이 각각 11.13, $9.31\%$로 tocopherol $9.28\%$보다 높았다. 활나물 부위별 추출물에 대한 hydroxy radical 소거 활성은 잎 추출물에서 $42.90\%$로 가장 높게 나타났고, 줄기, 종자, 뿌리 추출물은 각각 41.03, 37.80, $37.62\%$이었으며, 천연항산화제 인 tocopherol $32.38\%$ 이 가장 낮은 결과를 보여주었다. 종자 추출물의 hydrogen radical 소거능이 $53.51\%$로 가장 높게 나타났으며, tocopherol의 $53.81\%$와 비슷한 활성 수준을 보여 주었다. 따라서 활나물 부위 별 추출물의 항산화 효과를 측정한 결과 잎 추출물이 가장 항산화 활성이 높았으며, 활나물은 천연항산화제로의 개발 가능성이 있음을 확인할 수 있었다.

Keywords

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