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홍경천의 항산화 · 항발암 효과 연구

Anticarcinogenic and Antioxidant Effects of Rhodiola sachalinensis

  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechology Center for Biofunctional Material Industries, Silla University)
  • 발행 : 2005.11.01

초록

중국, 일본 및 한국에서 약용으로 이용되는 홍경천을 시료로 하여 추출, 각 용매별로 분획하여 생리활성 물질을 검색하였다. 4종의 암세포주인 HeLa, MCF-7, HT-29 및 HepG2를 이용하여 암세포성장억제실험을 한 결과 홍경천의 ethylether층인 RSMEE에서는 다른 층에 비해서 비교적 높은 암세포성장억제 효과가 나타났다. 특히 HepG2 세포주에서는 시료 최고첨가농도인 $500{\mu}g/mL$을 첨가하였을 때 $90\%$의 비교적 높은 암세포억제 효과를 보였으며, MCF-7 세포주에서도 비슷한 결과였다. 한편 HepG2 세포주에 대한 홍경천의 QR 유도활성 효과는 분획물의 농도를 증가시킬수록 증가하였으며 특히 hexane층인 RSMH에서는 시료 최종첨가농도인 $200{\mu}g/mL$에서 3.5배의 높은 효과를 보였다. 이와 같은 결과에서, 한방에서 여러가지 약용으로 쓰이는 홍경천은 ethylether층 즉 TSMEE에서 암세포 성장저지효과가 제일 컸고, 암예방 지수인 QR 유도활성은 hexane층인 RSMH이 제일 높았으며 RSMEE층은 그 다음으로 효과가 있었다. 홍경천의 항산화효과는 이미 항산화제로 널리 알려진 Vit. C의 효과와 비슷하였으며 $200{\mu}g/mL$의 홍경천 첨가시 첨 가전보다 암세포수 감소와 더 불어 형태학적으로 괴사가 일어나기 시작함을 관찰할 수 있었다. 이와 같은 여러 실험결과에의 하면 예로부터 민간요법에서 애용되어온 홍경천은 암예방 및 암세포 증식억제 효과가 있으며 항산화력도 매우 좋은 건강기능성 식품 소재로 추천할 수 있다고 생각된다.

In this study, we investigated the anticarcinogenic and antioxidative activities of Rhodiola sachalinensis (RS). Hexane (RSMH), ethylether (RSMEE), ethylacetate (RSMEA), butanol (RSMB), aqueous (RSMA) fractions and methanol extract (RSM) were screened for their growth inhibition effects using 3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on HepG2, HeLa, MCF-7 and HT-29 cells. The anticarcinogenic effects of RSMEE was most significant when tested on MCF-7 and HepG2 cell lines at the concentration of $500{\mu}g/mL$ which resulted about $84\%\;and\;90\%$ on MCF-7 and HepG2 cells, respectively. The quinone reductase (QR)-inducing activity of RSMH on HepG2 cells was 3.5 times higher compared with the control at the concentration of $200{\mu}g/mL$. Antioxidative activities of RSM, RSMEE, RSMEA and RSMB showed about $80\%$ of electron donating activity (EDA) which were very similar to that of vitamin C as a control. We observed morphological changes of shrinking and the blebbing of HepG2 cancer cell membranes depending on the concentration of RSMEE.

키워드

참고문헌

  1. Ong TM, Whang WZ, Stewatr S, Brockman HE. 1986. Chlorophyllin; a potent antimutagen against envitonmental and dietary complex mixture. Mutat Res 173: 111-115 https://doi.org/10.1016/0165-7992(86)90086-2
  2. Park BJ, Suh HS, Chung GS, Sohn JK. 1987. Studies on protoplast culture and fusion in cruciferae. Korean J Breed 19: 230-234
  3. Stavric B. 1994. Role of chemopreventers in human diet. Clin Biochem 27: 319-322 https://doi.org/10.1016/0009-9120(94)00039-5
  4. Reddy L, Odhav B, Bhoola K. 2003. Natural products for cancer prevention: a global perspective. Pharmacology & Therapeutics 99: 1-13 https://doi.org/10.1016/S0163-7258(03)00042-1
  5. Chung TH. 1974. Korean flora (herb part). Academybook, Seoul. p 283
  6. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbrott BJ, Mayo JG, Shoemaker RH, Boyd MR. 1988. Fesaibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
  7. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evalution of a tetrazolium baxed semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
  8. Steinkellner H, Rabot S, Freywald C, Nobis E, Scharf G, Chabicovsky M, Knasmuller S, Kassie F. 2001. Effect of crucifrous vegetable and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutat Res 480-481
  9. Prochaska HJ, Santamaria AB. 1988. Direct measurement NAD(P)H. Quinone reductase from cells cultured in microtiter wells. A screening assay for anticarcinogenic enzyme inducer. Anal Biochem 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  10. Jung MJ, Chung HY, Kang SS, Choi JH, Bae KS, Choi JS. 2003. Antioxidant activity from the stem bark of Albizzia julibrissin. Arch Pharm Res 23: 458-462
  11. Shim SM, Choi SW, Bae SJ 2001. Effects of Punica granatum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J Korean Soc Food Sci Nutr 30: 80-85
  12. Bae SJ. 2004. Anticarcinogenic effect of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  13. Bae SJ. 2002. The effects of anticarciogenic activity of Solanum tuberosum peel fractions. J Korean Soc Food Sci Nutr 31: 905-909 https://doi.org/10.3746/jkfn.2002.31.5.905
  14. Song EK, Kim JH, Kim JS, Cho H, Nan JX, Shon DH, Ko GI, Oh HC, Kim YC. 2003. Hepatoprotective phenolic constituents of Rhodiola sachalinensis on tacrine-induced cytotoxicity in Hep G2 cell. Phytother Res 17: 563-565 https://doi.org/10.1002/ptr.1166
  15. Lee MW, Lee YA, Park HM, Toh SH, Lee EJ, lang HD, Kim YH. 2000. Antioxidant phenolic compounds from the roots of Rhodiola sachalinensis A. Bor. Arch Pharm Res 23: 455-458 https://doi.org/10.1007/BF02976571

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