Effect on Endurance Improving of Mixture of the Extracts of Watermelon and Octacosanol

Octacosanol과 박과식물 추출물을 주성분으로 하는 혼합물의 지구력 증진 효과

  • 임현우 (중앙대학교 약학대학 생약학 교실) ;
  • 김성규 ((주)주신알앤디) ;
  • 이민원 (중앙대학교 약학대학 생약학 교실)
  • Published : 2005.12.01


Octacosanol is known to enhance endurance activities, control cholesterol in body and improve the function of cardiopulmonary. Citrulline, which is main compound of watermelon, is known to improve angiectasia through stimulating production of nitric oxide. To improve endurance activity swimming test on rats was carried out using four samples such as 1$\%$ octacosanol, citrulline, the extracts of barks of watermelon and products, mixture of 1$\%$ octacosanol and the extracts of barks of watermelon (6 : 4). Biochemical assays on the liver and serum of tested rats were also performed using commercial analysis kits. In result, it was shown that swimming time of III group increased by 26$\%$ and that of V group was increased by 22$\%$ at the swimming test. As a result of biological assays on the liver and serum of tested rats it was possible to confirm stability of toxicity When compared with creatine kinase of control group (549.11$\pm$39.15 U/l) citrulline (644.11 $\pm$50.67 U/l) and products group (646.00$\pm$46.99 U/l) were largely increased. When compared with inorganic phosphate of control group (12.01$\pm$0.75 mg/이), citrulline (13.03$\pm$0.94 mg/dl) and products group (12.90$\pm$0.55 mg/dl) showed similar results. Also, when compared with lactic acid and glucose of control group (152.91 $\pm$ 13.45, 103.00$\pm$ 8.69 mg/dl), citrulline (125.53$\pm$15.54, 83.75$\pm$7.29 mg/dl) and products group (135.26$\pm$11.50, 78.57$\pm$9.79 mg/dl) were largely decreased. As these test results, it was determined that 1$\%$ octacosanol and extracts of barks of watermelon had some effect of improving endurance activity. Futhermore, it was thought that it could be used as source of functional food.


  1. Lim, K. W. and Suh, H. J. : The evaluation methods for endurance performance in human. The Kor. J. of Exer. Nutr. 7(2), 149 (2003)
  2. Wei, R. and Cao, Z. : Effects of Radix astragali and Radix ginseng in enhancing the metabolism of human myocardial cell in vitro. Chung Kuo Chung Yao Tsa Chih 17, 173 (1992)
  3. Kazuko, K., Kumlko, A. and Yohel, H. : Free primary alcohols in oils and waxes from germs, kernels and other components of nuts, seeds, fruits and cereals. J. of the American Oil Chem. Soc. 68(11), 869 (1991) https://doi.org/10.1007/BF02660604
  4. Rapport, L. J. : Nutraceuticals (3) : octacosanol. Pharmaceut. J. 265, 170 (2000)
  5. Shimura, S., Hasegawa, T., Takano, S. and Suzuki, T. : Studis on the effect of octacosanol on motor endurance in mice. Nutr. Reprod. Internat. 36, 1029 (1987)
  6. Arruzazabala, M. L., Carbajal, D., Mas, R., Molina, V., Valdes, S. and Laguna, A. : Cholesterol-lowering effects of policosanol in rabbits. Biol. Res. 27, 205 (1994)
  7. Ann, E. S., Ann, B. C., Kim, W. S., Lee, H., Je, S. Y., Park, K. M. and Lee, S. W. : The effect of octacosanol administration in endurance exercise capacity. The Kor. J. of Exerc. Nutr. 3(2), 85 (1999)
  8. Cureton, T. K. : The physiological effects of wheat germ oil on human in exercise, Charles C. Thomas Publisher (1972)
  9. Kato, S., Karino, K., Hasegawa, J., Nagasaki, A., Eguchi, M. and Ichinose, T. : Octacosanol affects lipid metabolism in rats fed on a high fat diet. British J. of Nutr. 73, 433 (1995)
  10. Rimandoa, A. M. and Perkins-Veazie, P. M. : Determination of citrulline in watermelon rind. J. of Chromato. A 1078, 196 (2005) https://doi.org/10.1016/j.chroma.2005.05.009
  11. Takahisa, K., Zhang, W. Y., Tomomi, G. and Seiichi O. : Induction of citrulline-nitric oxide (NO) cycle enzymes and NO production in immunostimulated rat RPE-J cells. Exper. Eye Res. 76, 15 (2002) https://doi.org/10.1016/S0014-4835(02)00274-9
  12. Lieve, A., Van Geldre, Timmermans, J. P., Romain, A. and Lefebvre : L-Citrulline recycling by argininosuccinate synthetase and lyase in rat gastic fundus. Euro. J. of Pharmacol. 455, 149 (2002) https://doi.org/10.1016/S0014-2999(02)02584-0
  13. Vanaja, Paul: Evidence for the involvenment of L-citrulline but not nitric oxide in the proconvulsant action of the precursor L-arginine on picritoxin-induced convulsion in rats. Biochem. Pharmacol. 63, 2019 (2002) https://doi.org/10.1016/S0006-2952(02)00891-2
  14. Akashi, K., MiyakeI, C. and Yokota, A. : Citrulline, a novel compatible solute in drought-tolerant wild watermelon leaves, is an efficient hydroxyl radical scavenger. FEBS Lett. 508, 438 (2001) https://doi.org/10.1016/S0014-5793(01)03123-4
  15. Drewes, S. E., George, J. and Khan, F. : Recent findings on natural products with erectile-dysfunction activity. Phytochemistry 62(7), 1019 (2003) https://doi.org/10.1016/S0031-9422(02)00621-0
  16. Hong, S. G., Yang, D. S., Kang, B. J., Lee, H. S. and Yoon, Y. S. : Effects of formula (JR-22) maybe containing traditional herbs on maximal exercise perfoemance and antioxidant meterials in murine model. J. Kor. Soc. Food Sci. Nutr. 32(7), 1076 (2003) https://doi.org/10.3746/jkfn.2003.32.7.1076
  17. Do, J. H., Lee, S. K., Lee, J. W., See, E. O. and Kim, S. H. : Study on antioxidant and staminal activities of kejihongsamatang. J. of Ginseng Res. 24(4), 202 (2000)
  18. Lim, H. W., Kim, M. K., Kim, H. J., Shim, J. G., Kim, G. H., Choi, H. K. and Lee, M. W. : Quantitative determination of diarylheptanoid componds from genus alnus growing in Korea. Kor. J. of Pharmacogn. 35(4), 384 (2004)
  19. Bahrami, Gh., Mirzaeei, Sh. and Kiani, A. : Sensitive analytical method for Topiramate in human serum by HPLC with precolumn fluorescent derivatization and its application in human pharmacokinetic studies. J. of Chromato. B 813, 175 (2004) https://doi.org/10.1016/j.jchromb.2004.09.054
  20. Hans, M. H. van Eijk, Dennis, R. R., Peter, B. S. and Nicolaas, E. P. Deutz : Determination of amino acid isotope enrichment using liquid chromatography-mass spectrometry. Anal. Biochem. 271, 8 (1999) https://doi.org/10.1006/abio.1999.4112
  21. Yoshiji, O., Emi, S. and Isao, I. : Effect of oral octacosanol administration on hepatic triglyceride accumulation in rats with carbon tetrachloride-induced acute liver injury. Sch. Med., Fujita Health Univ., Toyoake, Japan. Igaku to Seibutsugaku 134(6), 185 (1997)
  22. Arruzazabala, M. L., Carbajal, D., Mas, R., Garcia, M. and Fraga, V. : Effects of policosanol on platelet aggregation in rats. Thrombosis Res. 69(3), 321 (1993) https://doi.org/10.1016/0049-3848(93)90030-R
  23. Folch, J., Less, M. and Sloanestanley, G. H. : A simple method for the isolation and purification of total lipid from animal tissues. J. of BioI. Chem. 497, 226 (1957)
  24. Millar, N. C. and Homsher, E. : The effects of phosphate and calcium on force generation in glycerinated rabbit skeletal muscle fibers. J. of BioI. Chem. 365, 20234 (1990)
  25. Walker, J. W., Lu, Z., Swartz, D. and Moss, R. L. : Thin filament modulation of cross-brige transition measured by photogeneration of Pi in skeletal muscle fibers. Biophys J. 59, 418a (1991)
  26. Paik, I. Y., Kim, J. K., Chun, Y. S. and Oho, H. J. : Verifying the validity of fatigue elements changes following absoluteexercise intensities. Kor. J. of Phys. Edu. 36, 218 (1997)
  27. Ronzoni, E. and Kerly, M. : The effects of pH on carbohydrate changes in isolated anaerobic frog miscle. J. of Biol. Chem. 103, 175 (1993)