Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Immunomodulatory Function of Murine NK Cell Activity by Alginate

  • Son Eun-Wha (Department of Pharmacognosy and Material development, Samcheok National University) ;
  • Yang Kwang-Hee (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University) ;
  • Rhee Dong-Kwon (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University) ;
  • Pyo Suhkneung (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University)
  • Published : 2005.11.01
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Abstract

The in vivo immunomodulatory function of the activity of murine natural killer (NK) cells induced by high mannuronic acid-containing alginate (HMA) was examined. HMA was injected i.p at doses of 25 and 100 mg/kg. The NK activity was 3 times higher with 100 mg/kg HMA than the baseline. In addition, in vitro studies of splenocytes cultured with HMA for 20 h showed a significant increase in NK activity at E:T ratio of 100: 1; a 160$\%$ and 210$\%$ increase at 10 and 100 $\mu$g/mL, respectively. There was a six fold increase in interferon-$\gamma$ production in a postculture of splenocytes with 100 $\mu$g/mL HMA. HMA had no suppressive effects on the lymphocyte function in the presence or absence of mitogens. This suggests that HMA is useful in cancer immunotherapy.

Keywords

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