Archives of Pharmacal Research

  • 제28권11호
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  • Pages.1302-1310
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Glucosylated Polyethylenimine as a Tumor-Targeting Gene Carrier

  • Park In-Kyu (School of Agricultural Biotechnology, Seoul National University) ;
  • Cook Seung-Eun (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim You-Kyoung (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim Hyun-Woo (School of Agricultural Biotechnology, Seoul National University) ;
  • Cho Myung-Haing (School of Agricultural Biotechnology, Seoul National University) ;
  • Jeong Hwan-Jeong (Department of Nuclear Medicine, Chonbuk National University) ;
  • Kim Eun-Mi (Department of Nuclear Medicine, Chonbuk National University) ;
  • Nah Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Bom Hee-Seung (Department of Nuclear Medicine, Chonnam National University School of Medicine) ;
  • Cho Chong-Su (School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2005.11.01
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초록

Glucosylated polyethylenimine (GPEI) was synthesized as a tumor-targeting gene carrier through facilitative glucose metabolism by tumor glucose transporter. Particle sizes of GPEI/DNA complex increased in proportion to glucose content of GPEI, whereas surface charge of the complex was not dependent on glucosylation, partially due to inefficient shielding of the short hydrophilic group introduced. GPEI with higher glucosylation (36 mol-$\%$) had no cytotoxic effect on cells even at polymer concentrations higher than 200 $\mu$g/mL. Compared to unglucosylated PEl. glucosylation induced less than one-order decrease of transfection efficiency. Transfection of GPEI/DNA complex into tumor cells possibly occurred through specific interaction between glucose-related cell receptors and glucose moiety of GPEI. Gamma imaging technique revealed GPEI/DNA complex was distributed in liver. spleen. and tumors.

키워드

참고문헌

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