Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Molecular Cloning and Analysis of the Genes in the Vicinity of Streptomyces griseus Trypsin (SGT) Gene from Streptomyces griseus ATCC10137

Streptomyces griseus ATCC10137에서 Trypsin 유전자 sprT의 주변 유전자군 분석

  • Chi Won-Jae (Department of Biological Science, Myongji University) ;
  • Kim Mi-Soon (Department of Biological Science, Myongji University) ;
  • Kim Jong-Hee (Department of Biological Science, Myongji University) ;
  • Kang Dae-Kyung (Resources Institute, Easy Bio System Inc.) ;
  • Hong Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (명지대학교 생명과학과) ;
  • 김미순 (명지대학교 생명과학과) ;
  • 김종희 (명지대학교 생명과학과) ;
  • 강대경 ((주)이지바이오시스템 중앙연구소) ;
  • 홍순광 (명지대학교 생명과학과)
  • Published : 2005.12.01
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Abstract

A 6.7kb DNA fragment containing the sprT gene encoding Streptomyces griseus trypsin (SGT) was cloned from Streptomyces griseus ATCC 10137, and the complete nucleotide sequence was determined. Nucleotide sequence and deduced amino acid or the EcoRI-HindIII fragment revealed the presence or the six complete ORFs containing the sprT gene and one incomplete ORF, which were named ORF1, SGT, ORF2, ORF3, ORF4, ORF5, and ORF6, respectively. ORF1 has homology with the oxidoreductases from several organisms. ORF2 and ORF3 show similarity with unknown proteins and transcription regulator that belongs to the ArsR family, respectively. ORF4 and ORF5 show homology with the peptidoglycan bound protein with LPXTG motif from Listeria monocytogenes and the membrane protein with transmembrane helix from several organisms, respectively. The last ORF, ORF6, shows homology with the lipoprotein from Streptomyces avermitilis.

Streptomyces griseus trypsin(SGT)을 코드하는 sprT 유전자를 포함하여 약 6.7 kb의 DNA 단편을 Streptomyces griseus ATCC 10137의 염색체 DNA로부터 클로닝 하여 염기서열을 결정하였다. 염기서열 분석 결과, 염색체 DNA를 EcoRI-HindIII 제한효소로 완전 분해하여 클로닝한 약6.7 kb의 단편에는 sprT유전자를 포함하여 총6개의 완전한 ORF (open reading frame)와 1개의 불완전한 ORF가 존재하는 것으로 밝혀졌으며, 순서대로 ORF1, SGT, ORF2, ORF3, ORF4, ORF5, ORF6로 명명하였다. 예상 단백질의 아미노산 서열 분석 결과, ORF1은 oxidoreductase, ORF3는 ArsR family의 transcription regulator, ORF4는 Listeria monocytogenes의 LPXTG motif를 갖는 peptidoglycan bound protein, ORF5는 transmembrane helix를 갖는 막단백질, ORF6는 Streptomyces avermitilis에서 보고된 lipoprotein과 높은 상동성을 보였으며, ORF2는 기능을 예측 할 수 없었다. 이와 같은 분석결과, sprT 유전자 주위에는 세포막이나 세포벽 구성성분을 코드하는 유전자가 존재하고 있으며, 따라서 SGT Pretense는 이러한 세포막 또는 세포벽 합성이나 분해과정에서 어떤 기능을 담당할 가능성 이 있는 것으로 추측되었다.

Keywords

References

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