Molecular Cloning and Analysis of the Genes in the Vicinity of Streptomyces griseus Trypsin (SGT) Gene from Streptomyces griseus ATCC10137

Streptomyces griseus ATCC10137에서 Trypsin 유전자 sprT의 주변 유전자군 분석

  • Chi Won-Jae (Department of Biological Science, Myongji University) ;
  • Kim Mi-Soon (Department of Biological Science, Myongji University) ;
  • Kim Jong-Hee (Department of Biological Science, Myongji University) ;
  • Kang Dae-Kyung (Resources Institute, Easy Bio System Inc.) ;
  • Hong Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (명지대학교 생명과학과) ;
  • 김미순 (명지대학교 생명과학과) ;
  • 김종희 (명지대학교 생명과학과) ;
  • 강대경 ((주)이지바이오시스템 중앙연구소) ;
  • 홍순광 (명지대학교 생명과학과)
  • Published : 2005.12.01

Abstract

A 6.7kb DNA fragment containing the sprT gene encoding Streptomyces griseus trypsin (SGT) was cloned from Streptomyces griseus ATCC 10137, and the complete nucleotide sequence was determined. Nucleotide sequence and deduced amino acid or the EcoRI-HindIII fragment revealed the presence or the six complete ORFs containing the sprT gene and one incomplete ORF, which were named ORF1, SGT, ORF2, ORF3, ORF4, ORF5, and ORF6, respectively. ORF1 has homology with the oxidoreductases from several organisms. ORF2 and ORF3 show similarity with unknown proteins and transcription regulator that belongs to the ArsR family, respectively. ORF4 and ORF5 show homology with the peptidoglycan bound protein with LPXTG motif from Listeria monocytogenes and the membrane protein with transmembrane helix from several organisms, respectively. The last ORF, ORF6, shows homology with the lipoprotein from Streptomyces avermitilis.

Streptomyces griseus trypsin(SGT)을 코드하는 sprT 유전자를 포함하여 약 6.7 kb의 DNA 단편을 Streptomyces griseus ATCC 10137의 염색체 DNA로부터 클로닝 하여 염기서열을 결정하였다. 염기서열 분석 결과, 염색체 DNA를 EcoRI-HindIII 제한효소로 완전 분해하여 클로닝한 약6.7 kb의 단편에는 sprT유전자를 포함하여 총6개의 완전한 ORF (open reading frame)와 1개의 불완전한 ORF가 존재하는 것으로 밝혀졌으며, 순서대로 ORF1, SGT, ORF2, ORF3, ORF4, ORF5, ORF6로 명명하였다. 예상 단백질의 아미노산 서열 분석 결과, ORF1은 oxidoreductase, ORF3는 ArsR family의 transcription regulator, ORF4는 Listeria monocytogenes의 LPXTG motif를 갖는 peptidoglycan bound protein, ORF5는 transmembrane helix를 갖는 막단백질, ORF6는 Streptomyces avermitilis에서 보고된 lipoprotein과 높은 상동성을 보였으며, ORF2는 기능을 예측 할 수 없었다. 이와 같은 분석결과, sprT 유전자 주위에는 세포막이나 세포벽 구성성분을 코드하는 유전자가 존재하고 있으며, 따라서 SGT Pretense는 이러한 세포막 또는 세포벽 합성이나 분해과정에서 어떤 기능을 담당할 가능성 이 있는 것으로 추측되었다.

Keywords

References

  1. Ahlert, J., E. Shepard, N. Lomovskaya, E. Zazopoulos, A. Staffa, B.O. Bachmann, K. Huang, L. Fonstein, A. Czisny, R.E. Whitwam, C.M. Farnet, and J.S. Thorson. 2002. The calicheamicin gene cluster and its iterative type I enediyne PKS. Science 297, 1173-1176 https://doi.org/10.1126/science.1072105
  2. Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403- 410 https://doi.org/10.1016/S0022-2836(05)80360-2
  3. Bentley, S.D., K.F. Chater, A.M. Cerdeno-Tarraga, G.L. Challis, N.R. Thomson, K.D. James, D.E. Harris, M.A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C.W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hronsby, S. Howarth, C.H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O'Neil, E. Rabbinowitsch, M.A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, D. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietzorrek, J. Woodward, B.G. Barrell, J. Parkhill, and D.A. Hopwood. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417, 141-147 https://doi.org/10.1038/417141a
  4. Capela, D., F. Barloy-Hubler, J. Gouzy, G. Bothe, f. Ampe, J. Batut, P. Boistard, A. Becker, M. Boutry, E. Cadieu, S. Dreano, S. Gloux, T. Godrie, A. Goffeau, D. Kahn, E. Kiss, V. Lelaure, D. Masuy, T. Pohl, D. Portetelle, A. Puehler, B. Purnelle, U. Ramsperger, C. Renard, P. Thebault, M. Vandenbol, S. Weidner, and F. Galibert. 2001. Analysis of the chromosome sequence of the legume symbiotic Sinorhizobium meliloti strain 1021. Proc. Natl. Acad. Sci. U.S.A. 98, 9877-9882
  5. Chi, W.-J., J.-M. Kim, S.-S. Choi, D.-K. Kang, and S.-K. Hong. 2001. Overexpression of SGPA and SGT induces morphological changes in Streptomyces lividans. J. Microbiol. Biotechnol. 11, 1077-1086
  6. Chi, W.-J., Y.-H. Kim, J.-H. Kim, D.-K. Kang, S.-S. Kang, J.-W. Suh, and S.-K. Hong. 2003. Streptomyces griseus Trypsin (SGT) has gelatinase activity and its proteolytic activity is enhanced by manganese. J. Microbiol. 41, 289-294
  7. Dodd I.B., and J.B. Egan. 1990. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucleic Acids Res. 18, 5019-5026 https://doi.org/10.1093/nar/18.17.5019
  8. Glaser, P., L. Frangeul, C. Buchrieser, A. Amend, F. Baquero, P. Berche, H. Bloecker, P. Brandt, T. Chakrabroty, A. Charbit, F. Chetouani, E. Couve, A. de Daruvar, P. Dehoux, E. Domann, G. Dominguez-Bernal, E. Duchaud, L. Durand, O. Dussurget, K.-D. Entian, H. Fishi, F. Garica-Del Portillo, P. Garrido, L. Gautier, W. Goevel, N. Gomez-Lopez, T. Hain, J. Hauf, D. Jackson, L.-M. Jones, U. Karst, J. Kreft, M. Kuhn, F. Kunst, G. Kurapkat, E. Madueno, A. Maitournam, J. Mata Vicente, F. Ng, G. Kurapkat, S. Novella, B. de Pablos, J.-C. Perez-Diaz, B. Remmel, M. Rose, C. Rusniok, T. Schlueter, N. Simoes, A. Tierrez, J.-A. Vazquez- Boland, H. Voss, J. Wehland, and P. Cossart. 2001. Comparative genomics of Listeria species. Science. 294, 849-852
  9. von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic. Acids. Res. 14, 4683-4690 https://doi.org/10.1093/nar/14.11.4683
  10. Hopwood, D.A., M.J. Bibb, K.F. Chater, T. Kieser, C.J. Bruton, H. M. Kieser, D.J. Lydiate, C.P. Smith, and J.M. Ward. 1985. Genetic manipulation of Streptomyces : a laboratory manual, The John Innes Foundation, Norwich, England
  11. Horinouchi S. 2002. A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus. Front Biosci. 7, 2045-2057 https://doi.org/10.2741/horinouc
  12. Ikeda, H., J. Ishikawa, A. Hanamoto, M. Shinose, H. Kikuchi, T. Shiba, Y. Sakaki, M. Hattori, and S. Omura. 2003. Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat. Biotechnol. 21, 526-31 https://doi.org/10.1038/nbt820
  13. Kaneko, T., Y. Nakamura, S. Sato, E. Asamizu, T. Kato, S. Sasamoto, A. Watanabe, K., Idesawa, A. Ishikawa, K. Kawashima, T. Kimura, Y. Kishida, C. Kiyokawa, M. Kohara, M. Matsumoto, A. Matsuno, Y. Mochizuki, S. Nakayama, N. Nakazaki, S. Shimpo, M. Sugimoto, C. Takeuchi, M. Yamada, and S. Tabata. 2000. Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti. DNA Res. 7, 331-338 https://doi.org/10.1093/dnares/7.6.331
  14. Kato J.-Y., W.-J. Chi, Y. Ohnishi, Soon-Kwang Hong, and S. Horinouchi. 2005. Transcriptional Control by A-Factor of Two Trypsin Genes in Streptomyces griseus. J. Bacteriol. 187, 286-295 https://doi.org/10.1128/JB.187.1.286-295.2005
  15. Katoh, T., N. Kikuchi, K. Nagata, and N. Yoshida. 1995. Cloning and expression of trypsin-like enzyme from Streptomyces fradiae for comparative analysis of functional regions of Streptomyces and mammalian trypsins. J. Ferment. Bioeng. 80, 440-445 https://doi.org/10.1016/0922-338X(96)80917-2
  16. Kim, I.S. and Lee, K.J. 1996. Chymotrypsin-like protease of Streptomyces exfoliatus SMF13, a potential agent in mycelial differentiation. Microbiology 142, 1797-1806 https://doi.org/10.1099/13500872-142-7-1797
  17. Kim, J.C., S.H. Cha, S.T. Jeong, S.K. Oh, and S.M. Byun. 1991. Molecular cloning and nucleotide sequence of Streptomyces griseus griseus trypsin gene. Biochem. Biophys. Res. Commun. 181, 707-713 https://doi.org/10.1016/0006-291X(91)91248-B
  18. Koo, B.J., K.H. Bae, S.M. Byun, and S.-K. Hong. 1998. Purification and characterization of Streptomyces griseus trypsin overexpressed in Streptomyces lividans. J. Microbiol. Biotechnol. 8, 333-340
  19. Rost B., R. Casadio, P. Fariselli, and C. Sander. 1995. Transmembrane helices predicted at 95% accuracy. Protein Sci. 4, 521-533 https://doi.org/10.1002/pro.5560040318
  20. Sambrook, J., E.F. Fritsch, and T. maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
  21. Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivityof progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  22. Tomono, A., Y. Tsai, Y. Ohnishi, and S. Horinouchi. 2005. Three chymotrypsin genes are members of the AdpA regulon in the A-factor regulatory cascade in Streptomyces griseus. J. bacteriol. 187, 6341-6353 https://doi.org/10.1128/JB.187.18.6341-6353.2005