Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of Microbial Community During the Anaerobic Dechlorination of Perchloroethylene and Trichloroethylene

Perchloroethylene과 Trichloroethylene의 혐기적 탈염소화 및 미생물 군집 분석

  • Lee Jae-Won (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Byung-Hyuk (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn Chi-Yong (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Hee-Sik (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon Byung-Dae (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh Hee-Mock (Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 이재원 (한국생명공학연구원 환경생명공학연구실) ;
  • 김병혁 (한국생명공학연구원 환경생명공학연구실) ;
  • 안치용 (한국생명공학연구원 환경생명공학연구실) ;
  • 김희식 (한국생명공학연구원 환경생명공학연구실) ;
  • 윤병대 (한국생명공학연구원 환경생명공학연구실) ;
  • 오희목 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2005.12.01
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Abstract

In this study, the anaerobic enrichment cultivation was performed with the sediments and the dredged soils from the cities of Ulsan, Masan, Yeosu, Gwangyang, Ansan and Seongnam. Acetate as an electron donor and PCE (perchloroethylene) or TCE (trichloroethylene) as an electron acceptor were injected into the serum bottle with an anaerobic medium. After the incubation of 12 weeks, the removal efficiency of PCE was highest at $70\%$ in the treatment with the sediment of Ulsan. Also, the bacterial community structure was analyzed by D-DGGE (double denatured gradient gel electrophoresis) through PCR-based 16S rDNA approaches. The dominant species id the anaerobic enrichment were found to belong to the genus of Desulfovibrio.

울산, 여수 등 공단지역의 토양, 하천의 저니, 해양의 준설토 둥을 이용하여 난분해성 염소화합물인 PCE (perchloroethylene)및 TCE (trichloroethylene)의 혐기성 탈염소화에 관련하는 미생물을 탐색하고 이들의 탈염소화 효율을 조사하였다. 혐기성 상호대사에 의한 탈염소화 효율을 조사하기 위해 전자공여체로 acetate를 사용하여 혐기성 회분식 실험을 실시하였으며, 이와 병행하여 분자생물학적인 기법인 16S rDNA의 PCR-Double Gradient DGGE (DG-DGGE)를 이용하여 미생물의 군집을 분석하였다. 그 결과 울산 태화강 및 여수 하남천의 저니를 접종한 경우 PCE는 $70\%,\;65\%$, TCE는 $50\%,\;45\%$의 높은 탈염소화 효율을 나타내었다. 또한 16S rDNA의 PCR을 이용한 DG-DGGE로 미생물 군집을 분석한 결과, 탈염소화 효율이 높은 지역의 저니에는 Desulfovibrio sup.의 미생물이 주로 존재함을 확인하였다.

Keywords

References

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